Lysosomal impairment-mediated autophagy dysfunction responsible for the vascular endothelial apoptosis caused by silica nanoparticle via ROS/PARP1/AIF signaling pathway

Abulikemu, Alimire; Zhao, Xinying; Qi, Yi; Liu, Yufan; Wang, Ji; Zhou, Wei; Duan, Huawei; Li, Yanbo; Sun, Zhiwei; Guo, Caixia

doi:10.1016/j.envpol.2022.119202

Cited by 21 publications

(12 citation statements)

References 79 publications

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“…It has been well-recognized that NPs would normally accumulate within the lysosome after cellular endocytosis. , Afterward, we evaluated the influence of DM-CaCO 3 @L-PEG NPs for lysosome. Interestingly, it was found that the intracellular red fluorescence signal from the lysosome was reduced after incubation with DM-CaCO 3 @L-PEG NPs (Figure c and Figure S6), indicating the damage of acidic lysosome, which was similar to previous published researches. , …”

Section: Resultssupporting

confidence: 89%

Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca²⁺/Cu²⁺ Dual-Ions Nano Trap for Breast Cancer Treatment

Guo,

Gao,

et al. 2024

ACS Nano

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Regarded as one of the hallmarks of tumorigenesis and tumor progression, the evasion of apoptotic cell death would also account for treatment resistance or failure during cancer therapy. In this study, a Ca 2+ /Cu 2+ dual-ion "nano trap" to effectively avoid cell apoptosis evasion by synchronously inducing paraptosis together with apoptosis was successfully designed and fabricated for breast cancer treatment. In brief, disulfiram (DSF)-loaded amorphous calcium carbonate nanoparticles (NPs) were fabricated via a gas diffusion method. Further on, the Cu 2+ -tannic acid metal phenolic network was embedded onto the NPs surface by self-assembling, followed by mDSPE-PEG/lipid capping to form the DSF-loaded Ca 2+ /Cu 2+ dual-ions "nano trap". The as-prepared nanotrap would undergo acid-triggered biodegradation upon being endocytosed by tumor cells within the lysosome for Ca 2+ , Cu 2+ , and DSF releasing simultaneously. The released Ca 2+ could cause mitochondrial calcium overload and lead to hydrogen peroxide (H 2 O 2 ) overexpression. Meanwhile, Ca 2+ /reactive oxygen speciesassociated mitochondrial dysfunction would lead to paraptosis cell death. Most importantly, cell paraptosis could be further induced and strengthened by the toxic dithiocarbamate (DTC)−copper complexes formed by the Cu 2+ combined with the DTC, the metabolic products of DSF. Additionally, the released Cu 2+ will be reduced by intracellular glutathione to generate Cu + , which can catalyze the H 2 O 2 to produce a toxic hydroxyl radical by a Cu + -mediated Fenton-like reaction for inducing cell apoptosis. Both in vitro cellular assays and in vivo antitumor evaluations confirmed the cancer therapeutic efficiency by the dual ion nano trap. This study can broaden the cognition scope of dual-ion-mediated paraptosis together with apoptosis via a multifunctional nanoplatform.

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Section: Resultssupporting

confidence: 89%

Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca²⁺/Cu²⁺ Dual-Ions Nano Trap for Breast Cancer Treatment

Guo,

Gao,

et al. 2024

ACS Nano

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“…In addition, it is known that such damage contributes to increased permeability of lysosome membranes, decreasing the lysosomal enzyme activity, increasing the ROS levels, and most importantly triggering apoptosis. 54 This was likely the cause of tumour death induced by ZnMnFe 2 O 4 –PEG–FA NPs.…”

Section: Resultsmentioning

confidence: 99%

Functionalized ZnMnFe₂O₄–PEG–FA nanoenzymes integrating diagnosis and therapy for targeting hepatic carcinoma guided by multi-modality imaging

Liu

Shi

et al. 2023

Nanoscale

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“…The results confirmed the synergistic effect of PTT and POD-like enzyme catalytic activity of AuNC@Fe 3 O 4 on lysosomal impairment. Additionally, lysosomal impairment may contribute to an increase in lysosomal membrane permeability, a decrease in lysosomal quantity, a disruption in lysosomal enzyme activities, an increase in ROS levels, and most importantly, the induction of cell apoptosis ( Abulikemu et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

A peroxidase-like magneto-gold nanozyme AuNC@Fe3O4 with photothermal effect for induced cell apoptosis of hepatocellular carcinoma cells in vitro

Shi

Liu

Wang

2023

Front. Bioeng. Biotechnol.

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Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed and malignant cancers worldwide. Conventional therapy strategies may not completely eradicate the tumor and may cause side effects during treatment. Nano-catalytic therapy, as a novel strategy, has attracted a great deal of attention. This study aimed to synthesize a multifunctional magneto-gold nanozyme AuNC@Fe3O4 and evaluate its anti-cancer potential in HepG2 cells in vitro. The characteristics of AuNC@Fe3O4 were assessed using a transmission electron microscope, dynamic light scattering, and energy-dispersive X-ray. The photothermal performance and peroxidase (POD)-like activity of AuNC@Fe3O4 were detected, using thermal camera and ultraviolet-visible spectrophotometer, respectively. The anti-cancer potential of AuNC@Fe3O4 was examined using cell counting kit-8, live/dead cell staining, and apoptosis analysis. Further research on HepG2 cells included the detection of intracellular reactive oxygen species (ROS) and lysosomal impairment. We observed that the AuNC@Fe3O4 had a small size, good photothermal conversion efficiency and high POD-like activity, and also inhibited cell proliferation and enhanced cell apoptotic ability in HepG2 cells. Furthermore, the AuNC@Fe3O4 enhanced ROS production and lysosomal impairment via the synergistic effect of photothermal and nano-catalytic therapies, which induced cell death or apoptosis. Thus, the magneto-gold nanozyme AuNC@Fe3O4 may offer a potential anti-cancer strategy for HCC.

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Lysosomal impairment-mediated autophagy dysfunction responsible for the vascular endothelial apoptosis caused by silica nanoparticle via ROS/PARP1/AIF signaling pathway

Cited by 21 publications

References 79 publications

Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca²⁺/Cu²⁺ Dual-Ions Nano Trap for Breast Cancer Treatment

Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca²⁺/Cu²⁺ Dual-Ions Nano Trap for Breast Cancer Treatment

Functionalized ZnMnFe₂O₄–PEG–FA nanoenzymes integrating diagnosis and therapy for targeting hepatic carcinoma guided by multi-modality imaging

A peroxidase-like magneto-gold nanozyme AuNC@Fe3O4 with photothermal effect for induced cell apoptosis of hepatocellular carcinoma cells in vitro

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Lysosomal impairment-mediated autophagy dysfunction responsible for the vascular endothelial apoptosis caused by silica nanoparticle via ROS/PARP1/AIF signaling pathway

Cited by 21 publications

References 79 publications

Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca2+/Cu2+ Dual-Ions Nano Trap for Breast Cancer Treatment

Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca2+/Cu2+ Dual-Ions Nano Trap for Breast Cancer Treatment

Functionalized ZnMnFe2O4–PEG–FA nanoenzymes integrating diagnosis and therapy for targeting hepatic carcinoma guided by multi-modality imaging

A peroxidase-like magneto-gold nanozyme AuNC@Fe3O4 with photothermal effect for induced cell apoptosis of hepatocellular carcinoma cells in vitro

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Product

Resources

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Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca²⁺/Cu²⁺ Dual-Ions Nano Trap for Breast Cancer Treatment

Apoptosis and Paraptosis Induced by Disulfiram-Loaded Ca²⁺/Cu²⁺ Dual-Ions Nano Trap for Breast Cancer Treatment

Functionalized ZnMnFe₂O₄–PEG–FA nanoenzymes integrating diagnosis and therapy for targeting hepatic carcinoma guided by multi-modality imaging