Lysophosphatidylcholine: A Novel Modulator of<i>Trypanosoma cruzi</i>Transmission

Silva-Neto, Mário A.C.; Carneiro, A.; Silva-Cardoso, Lívia; Atella, Geórgia C.

doi:10.1155/2012/625838

Cited by 13 publications

(9 citation statements)

References 85 publications

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“…In addition, lysophosphatidylcholine increases intracellular calcium concentrations in macrophages, ultimately enhancing parasite invasion. Finally, lysophosphatidylcholine inhibits nitric oxide production in macrophages stimulated by T. cruzi and thus interferes with the immune system of the vertebrate host [46].…”

Section: Discussionmentioning

confidence: 99%

Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics

et al. 2013

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Chagas disease is a trypanosomiasis whose causative agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous insects known as triatomines and affects a large proportion of South America. The digestive tract of the insect vectors in which T. cruzi develops constitutes a dynamic environment that affects the development of the parasite. Thus, we set out to investigate the chemical composition of the triatomine intestinal tract through a metabolomics approach. We performed Direct Infusion Fourier Transform Ion Cyclotron Resonance Mass Spectrometry on fecal samples of three triatomine species (Rhodnius prolixus, Triatoma infestans, Panstrongylus megistus) fed with rabbit blood. We then identified groups of metabolites whose frequencies were either uniform in all species or enriched in each of them. By querying the Human Metabolome Database, we obtained putative identities of the metabolites of interest. We found that a core group of metabolites with uniform frequencies in all species represented approximately 80% of the molecules detected, whereas the other 20% varied among triatomine species. The uniform core was composed of metabolites of various categories, including fatty acids, steroids, glycerolipids, nucleotides, sugars, and others. Nevertheless, the metabolic fingerprint of triatomine feces differs depending on the species considered. The variable core was mainly composed of prenol lipids, amino acids, glycerolipids, steroids, phenols, fatty acids and derivatives, benzoic acid and derivatives, flavonoids, glycerophospholipids, benzopyrans, and quinolines. Triatomine feces constitute a rich and varied chemical medium whose constituents are likely to affect T. cruzi development and infectivity. The complexity of the fecal metabolome of triatomines suggests that it may affect triatomine vector competence for specific T. cruzi strains. Knowledge of the chemical environment of T. cruzi in its invertebrate host is likely to generate new ways to understand the factors influencing parasite proliferation as well as methods to control Chagas disease.

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Section: Discussionmentioning

confidence: 99%

Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics

et al. 2013

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“…However, when added separately, both LPS and LPC might be signaling through TLR-4, triggering a pro-inflammatory phenotype. Until so far, LPC was just described in R. prolixus saliva [12, 13]. In our results, it is important to highlight that the induction of IL-12 and IL-6 production by saliva was much lower than by LPS, a classical TLR ligand.…”

Section: Discussionmentioning

confidence: 51%

“…This seems to be very important during blood-feeding, since the release of histamine by basophils and mast cells can induce inflammatory reaction with increased vascular permeability, plasma exudation and itching induction in the bite site [9–11]. More recently it has been shown that R. prolixus can secrete lysophosphatidylcholine (LPC), a lipid molecule present in saliva that may play a role in modulating macrophage and facilitating the transmission of T. cruzi to their hosts [12, 13]. …”

Section: Introductionmentioning

confidence: 99%

Effect of the saliva from different triatomine species on the biology and immunity of TLR-4 ligand and Trypanosoma cruzi-stimulated dendritic cells

et al. 2016

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BackgroundTriatomines are blood-sucking vectors of Trypanosoma cruzi, the causative agent of Chagas disease. During feeding, triatomines surpass the skin host response through biomolecules present in their saliva. Dendritic cells (DCs) play a crucial role in the induction of the protection to aggressive agents, including blood-sucking arthropods. Here, we evaluated if salivary components of triatomines from different genera evade the host immunity by modulating the biology and the function of LPS- or T. cruzi-stimulated DCs.MethodsSaliva of Panstrongylus lignarius, Meccus pallidipennis, Triatoma lecticularia and Rhodnius prolixus were obtained by dissection of salivary glands and the DCs were obtained from the differentiation of mouse bone marrow precursors.ResultsThe differentiation of DCs was inhibited by saliva of all species tested. Saliva differentially inhibited the expression of MHC-II, CD40, CD80 and CD86 in LPS-matured DCs. Except for the saliva of R. prolixus, which induced IL-6 cytokine production, TNF-α, IL-12 and IL-6 were inhibited by the saliva of the other three tested species and IL-10 was increased in all of them. Saliva per se, also induced the production of IL-12, IL-6 and IL-10. Only the saliva of R. prolixus induced DCs apoptosis. The presence of PGE2 was not detected in the saliva of the four triatomines studied. Finally, T. cruzi invasion on DCs is enhanced by the presence of the triatomine saliva.ConclusionsThese results demonstrate that saliva from different triatomine species exhibit immunomodulatory effects on LPS and T. cruzi-stimulated DCs. These effects could be related to hematophagy and transmission of T. cruzi during feeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1890-x) contains supplementary material, which is available to authorized users.

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“…). In the case of R. prolixus a wide array of different molecules are described: the gas nitric oxide, proteins such as apyrase and nitric‐oxide carrying molecules (nitrophorins) and lysophosphatidylcholine . Figure shows that these “salivary agents” block the activation of host innate immunity and inhibit vasoconstriction.…”

Section: Resultsmentioning

confidence: 99%

Triatominae biochemistry goes to school: Evaluation of a novel tool for teaching basic biochemical concepts of chagas disease vectors

Cunha

Cudischevitch

Carneiro

et al. 2014

Biochem Molecular Bio Educ

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We evaluate a new approach to teaching the basic biochemistry mechanisms that regulate the biology of Triatominae, major vectors of Trypanosoma cruzi, the causative agent of Chagas disease. We have designed and used a comic book, "Carlos Chagas: 100 years after a hero's discovery" containing scientific information obtained by seven distinguished contemporary Brazilian researchers working with Triatominaes. Students (22) in the seventh grade of a public elementary school received the comic book. The study was then followed up by the use of Concept Maps elaborated by the students. Six Concept Maps elaborated by the students before the introduction of the comic book received an average score of 7. Scores rose to an average of 45 after the introduction of the comic book. This result suggests that a more attractive content can greatly improve the knowledge and conceptual understanding among students not previously exposed to insect biochemistry. In conclusion, this study illustrates an alternative to current strategies of teaching about the transmission of neglected diseases. It also promotes the diffusion of the scientific knowledge produced by Brazilian researchers that may stimulate students to choose a scientific career.Copyright V C 2014 by The International Union of Biochemistry and Molecular Biology, 42(4): 323-330, 2014.

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Lysophosphatidylcholine: A Novel Modulator ofTrypanosoma cruziTransmission

Cited by 13 publications

References 85 publications

Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics

Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics

Effect of the saliva from different triatomine species on the biology and immunity of TLR-4 ligand and Trypanosoma cruzi-stimulated dendritic cells

Triatominae biochemistry goes to school: Evaluation of a novel tool for teaching basic biochemical concepts of chagas disease vectors

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