Lysophosphatidic Acid Reverts the β-Adrenergic Agonist-Induced Morphological Response in C6 Rat Glioma Cells

Koschel, Klaus; Tas, Piet W.L.

doi:10.1006/excr.1993.1133

Cited by 35 publications

(33 citation statements)

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“…A similar reversion of the morphological response has been obtained previously with thrombin, lysophosphatidic acid (LPA), or FCS (which contains LPA bound to serum albumin) (Koschel and Tas, 1993;. At low concentrations of S1P (for example, 20 nM) the reversion of the morphological response was only temporary, suggesting that S1P was metabolized or inactivated.…”

Section: ␤-Adrenergic Agonist-induced Morphological Changes Of C6 Celsupporting

confidence: 63%

“…1). As described previously the morphological response is also reversed by the addition of FCS, thrombin, or LPA, which is the component in FCS responsible for the reversion (Tas and Koschel, 1990;Koschel and Tas, 1993). The reversion obtained with LPA or thrombin is indistinguishable from the reversion obtained with S1P, although different receptors are activated.…”

Section: Discussionmentioning

confidence: 58%

“…2 and also ref. Tas and Koschel, 1990;Koschel and Tas, 1993). Second, inhibition of the Ca 2ϩ signal by pretreatment of the cells with PMA also abolished the reversion of the morphological response ( Fig.…”

Section: Discussionmentioning

confidence: 90%

“…3 and also ref. Tas and Koschel, 1990;Koschel and Tas, 1993). Third, depletion of internal Ca 2ϩ stores or complex formation of extracellular Ca 2ϩ with EGTA strongly suppresses the morphological reversion.…”

Section: Discussionmentioning

confidence: 96%

“…When added to the morphologically changed astrocyte-like cells, these were reverted to the fibroblast-like morphology in about 10-30 min (Tas and Koschel, 1990;Cavanaugh et al, 1990). The component in the serum responsible for this reversion is lysophosphatidic acid (LPA), a novel phospholipid signalling molecule with growth factor-like effects (Koschel and Tas, 1993;.…”

Section: Introductionmentioning

confidence: 98%

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Sphingosine-1-phosphate induces a Ca2+ signal in primary rat astrocytes and a Ca2+ signal and shape changes in C6 rat glioma cells

Tas

Koschel

1998

J. Neurosci. Res.

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Treatment of rat glioma C6 cells with the beta-adrenergic agonist L-isoproterenol leads to a rise in cAMP level and a subsequent change in cell morphology from an epithelial to an astrocytic type of appearance. This morphological response is reverted by the addition of sphingosine-1-phosphate (S1P) with an EC50 of 10 nM. In rat glioma C6 cells loaded with the Ca2+ indicator Fura-2, S1P evoked Ca2+ release from internal stores and Ca2+ influx from the external medium. Half-maximal stimulation of the Ca2+ increase was 10-20 nM. A similar Ca2+ signal was observed in primary rat astrocytes loaded with the Ca2+ indicator fluo-3. Pretreatment of the C6 cells with PMA (162 nM) prevented both the S1P-induced Ca2+ increase and the morphological reversion. Ca2+ ions therefore seem essential for the morphological reversion by S1P. Pretreatment of the cells with the Clostridium botulinum C3 exoenzyme did not affect the reversion of the morphological response by S1P, indicating that the small GTP-binding protein Rho is not involved in the S1P-induced reversion.

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Section: ␤-Adrenergic Agonist-induced Morphological Changes Of C6 Celsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Sphingosine-1-phosphate induces a Ca2+ signal in primary rat astrocytes and a Ca2+ signal and shape changes in C6 rat glioma cells

Tas

Koschel

1998

J. Neurosci. Res.

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Effects of GM-CSF and ordinary supplements on the ramification of microglia in culture: A morphometrical study

Fujita¹,

Tanaka²,

Toku³

et al. 1996

Glia

115

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Microglia transform from ameboid to ramified cells during development and display an ameboid appearance again under certain pathological conditions. Some cytokines produced by astrocytes may be responsible for the microglial transformation. In the present study, we compared the effects of cytokines, granulocyte/macrophage colony‐stimulating factor (GM‐CSF), macrophage colony‐stimulating factor (M‐CSF), and interleukin‐3 (IL‐3) on the morphology of rat cultured microglia. For quantitative evaluation, we employed “transformation index” as calculated by (perimeter of cell)2/4 π (cell area). GM‐CSF facilitated the ramification of cultured rat microglia, which was effectively induced in a serum‐free medium. However, M‐CSF and IL‐3 did not induce the ramification. A certain serum adhesion protein (possibly vitronectin) as well as other high molecular weight substances in fetal calf serum inhibited the GM‐CSF‐induced microglial ramification. Among ordinary supplements for a chemically defined medium, progesterone, insulin, and a high concentration of glucose suppressed the ramification. These findings suggest that GM‐CSF may be involved in microglial ramification and that many kinds of supplements that are added to culture media profoundly affect the morphology of microglial cells. © 1996 Wiley‐Liss, Inc.

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Lysophosphatidic acid depletes intracellular calcium stores different from those mediating capacitative calcium entry in C6 rat glioma cells

Hildebrandt

1997

Glia

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Lysophosphatidic acid (LPA) functions as an extracellular lipid mediator stimulating phospholipase C and affecting the structure of the cytoskeleton in several cell types. In rat glioma C6 cells, LPA mobilizes calcium from intracellular calcium stores and reverts morphological changes induced by elevated cytosolic cAMP‐concentrations. Here we show that LPA‐stimulation of C6 cells loaded with the calcium‐sensitive fluorescent dye indo‐1 results in calcium release from a subset of intracellular calcium stores that are not sensitive to the tumor promoter thapsigargin and do not overlap with calcium stores depleted during purinergic receptor stimulation with ATP. Furthermore, depletion of LPA‐sensitive calcium stores does not induce capacitative calcium entry from the extracellular space into the cytosol to the same extent as ATP. These results indicate that inositol phosphate signaling induced by LPA or ATP may differ in kinetics or in spatial organisation within the cell. This may represent a possible explanation for the previous observation that only LPA, but not other calcium‐mobilizing agonists, reverts cAMP‐induced changes in the cytoskeletal organization in C6 cells. GLIA 19:67–73, 1997. © 1997 Wiley‐Liss, Inc.

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Lysophosphatidic Acid Reverts the β-Adrenergic Agonist-Induced Morphological Response in C6 Rat Glioma Cells

Cited by 35 publications

References 0 publications

Sphingosine-1-phosphate induces a Ca2+ signal in primary rat astrocytes and a Ca2+ signal and shape changes in C6 rat glioma cells

Sphingosine-1-phosphate induces a Ca2+ signal in primary rat astrocytes and a Ca2+ signal and shape changes in C6 rat glioma cells

Effects of GM-CSF and ordinary supplements on the ramification of microglia in culture: A morphometrical study

Lysophosphatidic acid depletes intracellular calcium stores different from those mediating capacitative calcium entry in C6 rat glioma cells

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