Lysophosphatidic Acid Modulates the Regenerative Responses of Human Gingival Fibroblasts and Enhances the Actions of Platelet‐Derived Growth Factor

Cerutis, D. Roselyn; Dreyer, A; Cordini, Franco; McVaney, Timothy P.; Mattson, John S.; Parrish, Lawrence C.; Romito, Laura M.; Huebner, Gene R.; Jabro, M H

doi:10.1902/jop.2004.75.2.297

Cited by 18 publications

(34 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sugiura et al [16] first reported the presence of significant amounts of LPAs in normal human saliva, and these LPA levels were comparable to LPA levels in plasma.At these physiological levels, LPAs were shown to accelerate the growth and enhance the survival of cells derived from human esophagus, pharynx, and tongue.In addition, the LPA receptors (LPAR) LPA1, LPA2, and LPA3 are expressed by human gingival fibroblasts (GF) and periodontal ligament fibroblasts (PDLF)[14, 17]. Therefore, several lines of evidence support a role for LPAs in the pathogenesis of periodontal diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Intracellular calcium levels increasein PDLF and GF in response to saturated LPA species (LPA 18:0 and LPA 16:0), whereas the unsaturated LPA species (LPA 18:1) does not significantly stimulate intracellular calcium production, especially in PDLFs [17]. Because of the differences in the responses produced by various LPAs, it is thus important to detect individual LPA species rather than total LPAs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative determination of lysophosphatidic acids (LPAs) in human saliva and gingival crevicular fluid (GCF) by LC–MS/MS

Bathena

Huang

Nunn

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Lysophosphatidic acid (LPA) is a phospholipid mediator that plays multiple cellular functions by acting through G protein-coupled LPA receptors.LPAs are known to be key mediators in inflammation,and several lines of evidence suggest a role for LPAs in inflammatory periodontal diseases. A simple and sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method has been developed and validated to quantify LPA species (LPA 18:0, LPA 16:0, LPA 18:1 and LPA 20:4) in human saliva and gingival crevicular fluid (GCF). LPA 17:0 was used as an internal standard and the LPA species were extracted from saliva by liquid-liquid extraction using butanol. Chromatography was performed using a Macherey-Nagel NUCLEODUR® C8 Gravity Column (125mm × 2.0mm ID) with a mixture of methanol/water: 75/25 (v/v) containing 0.5% formic acid and 5mM ammonium formate (mobile Phase A) and methanol/water : 99/0.5 (v/v) containing 0.5% formic acid and 5mM ammonium formate (mobile phase B) at a flow rate of 0.5 mL/min. LPAs were detected by a linear ion trap-triple quadrupole mass spectrometer with a total run time of 8.5 min. The limit of quantification (LOQ) in saliva was 1 ng/mL for all LPA species and the method was validatedover the range of 1-200 ng/mL. The method was validated in GCF over the ranges of 10-500 ng/mL for LPA 18:0 and LPA 16:0, and 5-500 ng/mL for LPA 18:1 and LPA 20:4. This sensitive LC-MS/MS assay was successfully applied to obtain quantitative data of individual LPA levels from control subjects and patients with various periodontal diseases. All four LPA species were consistently elevated in samples obtained from periodontal diseases, which supports a role of LPAs in thepathogenesis of periodontal diseases.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative determination of lysophosphatidic acids (LPAs) in human saliva and gingival crevicular fluid (GCF) by LC–MS/MS

Bathena

Huang

Nunn

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

show abstract

“…Signaling by lysophosphatidic acid (LPA) and PDGF is activated in human cancer (20)(21)(22) and in wound healing (23)(24)(25), where these factors seem to stimulate cell migration, as was shown in cultured cells (26,27). Acquisition of a motile phenotype is a prerequisite for invasion and metastasis and thus important in promoting tumor progression.…”

Section: Introductionmentioning

confidence: 99%

Combined Lysophosphatidic Acid/Platelet-Derived Growth Factor Signaling Triggers Glioma Cell Migration in a Tenascin-C Microenvironment

Lange

Kammerer

Saupe³

et al. 2008

Cancer Research

View full text Add to dashboard Cite

The antiadhesive extracellular matrix molecule tenascin-C abrogates cell spreading on fibronectin through competitive inhibition of syndecan-4, thereby preventing focal adhesion kinase (FAK) activation and triggering enhanced proteolytic degradation of both RhoA and tropomyosin 1 (TM1). Here, we show that simultaneous signaling by lysophosphatidic acid (LPA) and platelet-derived growth factor (PDGF) initiates glioma cell spreading and migration through syndecan-4-independent activation of paxillin and FAK and by stabilizing expression of RhoA, TM1, TM2, and TM3. By using gene silencing methods, we show that paxillin, TM1, TM2, and TM3 are essential for LPA/PDGF-induced cell spreading on a fibronectin/tenascin-C (FN/TN) substratum. LPA/PDGFinduced cell spreading and migration on FN/TN depends on phosphatidylinositol 3-kinase, RhoKinase, and mitogenactivated protein kinase/extracellular signal-regulated kinase kinase 1/2 but is independent of phospholipase C and Jun kinase. RNA microarray data reveal expression of tenascin-C, PDGFs, LPA, and the respective receptors in several types of cancer, suggesting that the TN/LPA/PDGF axis exists in malignant tumors. These findings may in turn be relevant for diagnostic or therapeutic applications targeting cancer. [Cancer Res 2008;68(17):6942-52]

show abstract

“…Lysophosphatidic acid (LPA), an abundant serum factor, has been shown to stimulate wound closure (4,25,43). We tested LPA and found that wound closure in EGFϩLPA-treated cultures was significantly less than wound closure in cultures treated with EGFϩserum (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In the absence of serum, EGF induces CV-1 cell scattering, whereas in its presence, EGF promotes coordinated wound closure. Furthemore, EGFϩLPA synergistically stimulate mitogenesis in airway smooth muscle cells (5) and LPAϩPDGF-BB synergistically enhance human gingival fibroblast migration in scrape wound assays (4). In MDCK cells, hepatocyte growth factor (HGF) promotes scattering of isolated colonies and coordinated migration of wounded, polarized monolayers cultured on permeable substrates (1).…”

Section: Discussionmentioning

confidence: 99%

An in vitro model based on cell monolayers grown on the underside of large- pore filters in bicameral chambers for studying thyrocyte-lymphocyte interactions

Estienne¹,

Brisbarre²,

Blanchin³

et al. 2004

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

In the processes underlying thyroid autoimmunity, thyrocytes probably act as antigen-presenting cells exposing T-cell epitopes to intrathyroid lymphocytes. To study the interactions between lymphocytes and thyrocytes, which are arranged in a tight, polarized monolayer, we developed a new in vitro model based on human thyrocytes grown on the underside of a filter placed in a bicameral chamber. Thyrocytes from Graves' disease glands were plated onto the upper face of a 8-mum-pore polyethylene terephthalate culture insert filter placed in the inverted position and grown for 24 h before the insert was returned to the normal position for a week in the cell culture plate wells. Thyrocytes grown in the presence of thyroid stimulating hormone, forming a homogeneous monolayer on the underside of the filter, reached confluence after 8 days in vitro. The cells developed a transepithelial electrical resistance >1,000 Omega.cm(2), and the ZO-1 tight junction protein showed a junctional pattern of distribution. Thyrocytes showed a polarized pattern of thyroperoxidase and thyroid stimulating hormone receptor expression in the apical and basolateral positions, respectively. They were also found to aberrantly express DR class II human leukocyte antigen and an Fc immunoglobulin receptor (FcgammaRIIB2) in the basolateral and apical positions, respectively. Autologous intrathyroidal T lymphocytes cocultured for 24 h across the filter with the thyrocyte monolayer proliferated and remained in the upper chamber without any leakage occurring through the epithelial barrier, which makes this model particularly suitable for studying the cell-cell interactions involved in antigen processing.

show abstract

Lysophosphatidic Acid Modulates the Regenerative Responses of Human Gingival Fibroblasts and Enhances the Actions of Platelet‐Derived Growth Factor

Cited by 18 publications

References 33 publications

Quantitative determination of lysophosphatidic acids (LPAs) in human saliva and gingival crevicular fluid (GCF) by LC–MS/MS

Quantitative determination of lysophosphatidic acids (LPAs) in human saliva and gingival crevicular fluid (GCF) by LC–MS/MS

Combined Lysophosphatidic Acid/Platelet-Derived Growth Factor Signaling Triggers Glioma Cell Migration in a Tenascin-C Microenvironment

An in vitro model based on cell monolayers grown on the underside of large- pore filters in bicameral chambers for studying thyrocyte-lymphocyte interactions

Contact Info

Product

Resources

About