Lysophosphatidic acid improves porcine oocyte maturation and embryo development in vitro

Zhang, Jin Yu; Jiang, Yong; Lin, Tao; Kang, Jung Won; Lee, Jae Eun; Jin, Dong Il

doi:10.1002/mrd.22447

Cited by 40 publications

(34 citation statements)

References 60 publications

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“…Similar results were obtained by Zhang et al. () in pigs, who demonstrated that LPA improved oocyte maturation via the MAPK pathway. The authors also documented the beneficial influence of LPA on porcine cytoplasmic oocyte maturation.…”

Section: Discussionsupporting

confidence: 90%

Lysophosphatidic acid expression in theca cells depends on the type of bovine ovarian follicle

Sinderewicz

Grycmacher

Boruszewska

et al. 2016

Reprod Domestic Animals

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Lysophosphatidic acid (LPA) exerts various actions on the mammalian reproductive system. In cows, LPA stimulates the synthesis and secretion of luteotropic factors in the ovary, which affects the growth and development of ovarian follicles. The role of LPA in granulosa cells, oocyte and oocyte-cumulus complex (COC) has previously been investigated; but its role in the theca layer, which is an important structural and functional component of the ovarian follicle, is still unclear. The goal of this study was to investigate the expression of LPA in theca cells originating from different bovine ovarian follicle types. Theca cells were separated from healthy, transitional and atretic ovarian follicles, based on intrafollicular estradiol: progesterone ratios. LPA concentration in the follicular fluid (FF) in different follicle types was measured, and expression of the enzymes responsible for LPA synthesis (autotaxin [AX], phospholipase A2 [PLA2]) and receptors for LPA (LPAR1-4) were determined. The obtained results confirmed the follicle-type dependent presence of LPA in the FF of the bovine ovarian follicles. The highest concentration of LPA was detected in follicles classified as healthy and dominant. LPAR1-4, PLA2 and AX expression in theca cells in all of the types of follicles examined were detected at mRNA and protein level. These results suggest that theca cells can be a source of LPA synthesis other than granulosa cells and COCs, as well as the target for its action in the bovine ovarian follicle, with PLA2 and LPAR4 playing major roles in LPA synthesis and action.

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Section: Discussionsupporting

confidence: 90%

Lysophosphatidic acid expression in theca cells depends on the type of bovine ovarian follicle

Sinderewicz

Grycmacher

Boruszewska

et al. 2016

Reprod Domestic Animals

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“…Similar results were obtained by Zhang et al. () in pig, who demonstrated that LPA improved oocyte maturation via the MAPK pathway. In the present study, the highest concentration of the LPA was documented in the infundibulum in the periovulatory stage of the oestrous cycle (Fig.…”

Section: Discussionsupporting

confidence: 90%

“…In in vitro studies, it was documented that the supplementation of the maturation medium with LPA raises bovine oocyte maturation rates, supports the expression of the developmental competence related factors during oocyte maturation and subsequently affects gene expression profile at the blastocyst stage (Boruszewska et al 2015). Similar results were obtained by Zhang et al (2015) in pig, who demonstrated that LPA improved oocyte maturation via the MAPK pathway. In the present study, the highest concentration of the LPA was documented in the infundibulum in the periovulatory stage of the oestrous cycle (Fig.…”

Section: Discussionmentioning

confidence: 55%

“…Furthermore, Jo et al (2014) proved that the addition of LPA to the maturation medium enhanced the fertilization process in mouse. What is more, it has been shown that the supplementation of the maturation medium with LPA, during in vitro oocyte maturation, increased the oocyte developmental potential in bovine (Boruszewska et al 2015), porcine (Zhang et al 2015), or hamster (Hinokio et al 2002) oocytes. On the other hand, Boruszewska et al (2014) demonstrated mRNA expression of LPARs, ATX and PLA2 in the in vitro matured bovine oocytes and cumulus cells.…”

Section: Discussionmentioning

confidence: 98%

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Lysophosphatidic Acid Synthesis and its Receptors’ Expression in the Bovine Oviduct During the Oestrous Cycle

Sinderewicz

Grycmacher

Boruszewska

et al. 2016

Reprod Domestic Animals

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Lysophosphatidic acid (LPA) is a naturally occurring simple phospholipid which in the bovine reproductive system can be produced in the endometrium, corpus luteum, ovarian follicle and embryo. In this study, we examined the possibility that LPA receptors are expressed, and LPA synthesized, in the bovine oviduct. We found that the concentration of LPA was highest in infundibulum in the follicular phase of the oestrous cycle and was relatively high during the early-luteal phase in all examined parts of the oviduct. We also documented that LPA synthesis engages both available pathways for LPA production. The autotaxin (ATX) protein expression was significantly higher in the infundibulum compared to the isthmus during the follicular phase of the oestrous cycle. During the early-luteal phase of the oestrous cycle, ATX and phospholipase A2 (PLA2) protein expression was highest in ampulla, although the expression of LPARs was not as dynamic as LPA concentration in the oviduct tissue, and we presume that in the bovine oviduct, the most abundantly expressed receptor is LPAR2. In conclusion, our results indicate that the bovine oviduct is a site of LPA synthesis and a target for LPA action in the bovine reproductive tract. We documented that LPAR2 is the most abundantly expressed in the bovine oviduct. We hypothesize that in the bovine oviduct, LPA may be involved in the transport of gametes, fertilization and cellular signalling between the oviduct and cumulus-oocyte complex.

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“…Studies have shown that both in vivo and in vitro factors including hormones and growth factors can influence the competence of oocytes for maturation, growth, and nuclear and cytoplasmic development (van den Hurk & Zhao, ; Zhang et al, ). In particular, researchers have suggested that one desirable approach for the improvement of IVM systems is the application of cell‐based co‐culture systems including oviduct, cumulus, and granulosa cells to obtain higher yields of better quality oocytes (Feng, Shi, Yang, & Wang, ; Kidson et al, ; Lin et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of co‐culture human endothelial progenitor cells with porcine oocytes during maturation and subsequent embryo development of parthenotes in vitro

Lee

Kim

et al. 2018

Molecular Reproduction Devel

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Human endothelial progenitor cells (EPCs) have been applied to regenerative medicine for their roles in angiogenesis as well as neovascularization, and these angiogenetic functions have beneficial effects on maturation of ovarian follicles. However, little information is available on whether EPCs on culture systems affect oocyte maturation and subsequent embryo development. Therefore, the objective of this study was to investigate the effect of EPC co-culture on porcine oocytes during in vitro maturation (IVM) and subsequent embryo development, and to examine gene expression in cumulus cells, oocytes and blastocysts. The effect of co-culture using EPC on porcine oocyte IVM was investigated. Oocytes were activated using electrical stimulation and embryo developmental competence was estimated. The expression of the genes related to cumulus expansion, oocyte maturation, embryo development, and apoptosis were analyzed. In result, there was a significantly increased maturation rate in EPC group compared with control (p < 0.05). Also, oocytes co-cultured with EPCs exhibited significantly improved blastocyst formation rates (p < 0.05). The expression of mRNAs associated with cumulus expansion and apoptosis in cumulus cells was significantly up-regulated in EPC group. Also, markedly increased levels of GDF9, BMP15, and BCL2 were observed in oocytes from the EPC group. Blastocysts in the co-culture group showed significantly higher SOX2, OCT4, and NANOG levels. In conclusion, co-culturing porcine oocytes with EPCs improves their maturation by regulating genes involved in cumulus cell expansion, oocyte maturation, and apoptosis. Moreover, EPC co-culture during IVM enhanced embryo development as shown by increased blastocyst formation rate and pluripotency-related gene expression.

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Lysophosphatidic acid improves porcine oocyte maturation and embryo development in vitro

Cited by 40 publications

References 60 publications

Lysophosphatidic acid expression in theca cells depends on the type of bovine ovarian follicle

Lysophosphatidic acid expression in theca cells depends on the type of bovine ovarian follicle

Lysophosphatidic Acid Synthesis and its Receptors’ Expression in the Bovine Oviduct During the Oestrous Cycle

Effect of co‐culture human endothelial progenitor cells with porcine oocytes during maturation and subsequent embryo development of parthenotes in vitro

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