Lysophosphatidic acid enhances airway response to acetylcholine in guinea pigs

Protein Kinase Cδ Mediates Lysophosphatidic Acid-induced NF-κB Activation and Interleukin-8 Secretion in Human Bronchial Epithelial Cells

“…LPA is a potent bioactive lipid that mediates several cellular responses such as proliferation, differentiation, suppression of apoptosis, tumor metastasis, and cytoskeletal reorganization (15,(21)(22)(23)(24)(25)(26)(27)(28). LPA mediates the broad range of cellular responses through the G-protein-coupled receptors LPA 1 /Edg-2, LPA 2 /Edg-4, and LPA 3 /Edg-7 (19).…”

“…Studies in our laboratory have revealed that S1P stimulates significant production and secretion of IL-8 in a human bronchial epithelial cell line (BEAS-2B) (21) and that LPA is capable of transactivating platelet-derived growth factor receptor-␤ in HBEpCs (20). In guinea pigs, administration of exogenous LPA into the airway enhances the response to acetylcholine (22) and infiltration of neutrophils (23). Other reports have shown that LPA induces airway smooth muscle contractility (24), proliferation (25), and transcription factor activation (26).…”

Airway Smooth Muscle in Asthma Symptoms: Culprit but Maybe Innocent

“…Many investigators have focused on this strategy. Most studies were performed using freshly dissected ASM strips or bronchial rings from which isometric force was measured ex vivo (12,32,38,48,71,87,98,122,135,156,173,174,184,185,195,203,204,215,230). In vitro preparations (i.e., cell culture) have also been used on occasions (205).…”

Lysophosphatidic Acid (LPA) Induces Plasma Exudation and Histamine Release in Mice via LPA Receptors

“…Administration of LPA in vivo induces airway hyperresponsiveness (1), itch-scratch responses (ISRs) (2), infiltration and activation of eosinophils and neutrophils (3), vascular remodeling (4), and nociceptive flexor responses (5). Previously, we documented that LPA induced histamine release (6).…”