Lysogenic Transfer of
            <i>mef</i>
            (A) and
            <i>tet</i>
            (O) Genes Carried by Φm46.1 among Group A Streptococci

Luca, Maria Chiara Di; D'Ercole, Stefania; Petrelli, Dezemona; Prenna, Manuela; Ripa, Sandro; Vitali, Luca A.

doi:10.1128/aac.01318-09

Cited by 19 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacteriophages can also transfer inducible antibiotic resistance genes [13]. Phages are associated with different inducible macrolide resistance genes and cassettes carried by transposons in S. pneumoniae and other Gram-positive species [14]. β-lactamases can be disseminated in staphylococci by bacteriophages [15].…”

Section: Gene Pool For Inducible Antibiotic Resistance Determinants: mentioning

confidence: 99%

“…β-lactamases can be disseminated in staphylococci by bacteriophages [15]. Antibiotic stress can induce the lysogenic transfer of bacteriophages and therefore promote dissemination of resistance through induction of an SOS-like response [14]. Similarly, horizontal gene transfer can be induced by DNA-damaging compounds in Bacillus subtilis [16] and by antibiotic stress in S. pneumoniae [17].…”

Section: Gene Pool For Inducible Antibiotic Resistance Determinants: mentioning

confidence: 99%

See 1 more Smart Citation

Acquired Inducible Antimicrobial Resistance in Gram-Positive Bacteria

2012

View full text Add to dashboard Cite

A major contributor to the emergence of antibiotic resistance in Gram-positive bacterial pathogens is the expansion of acquired, inducible genetic elements. Although acquired, inducible antibiotic resistance is not new, the interest in its molecular basis has been accelerated by the widening distribution and often ‘silent’ spread of the elements responsible, the diagnostic challenges of such resistance and the mounting limitations of available agents to treat Gram-positive infections. Acquired, inducible antibiotic resistance elements belong to the accessory genome of a species and are horizontally acquired by transformation/recombination or through the transfer of mobile DNA elements. The two key, but mechanistically very different, induction mechanisms are: ribosome-sensed induction, characteristic of the macrolide–lincosamide–streptogramin B antibiotics and tetracycline resistance, leading to ribosomal modifications or efflux pump activation; and resistance by cell surface-associated sensing of β-lactams (e.g., oxacillin), glycopeptides (e.g., vancomycin) and the polypeptide bacitracin, leading to drug inactivation or resistance due to cell wall alterations.

show abstract

Section: Gene Pool For Inducible Antibiotic Resistance Determinants: mentioning

confidence: 99%

Section: Gene Pool For Inducible Antibiotic Resistance Determinants: mentioning

confidence: 99%

Acquired Inducible Antimicrobial Resistance in Gram-Positive Bacteria

2012

View full text Add to dashboard Cite

show abstract

“…Although transduction can also be observed in the lytic phages, most research supports the idea that only temperate phages transfer the antibiotic resistance gene from one bacterial host to the others. 84,[96][97][98][99] Given our current understanding of lytic and lysogenic phages, lytic phages have been favoured for use in phage therapy.…”

Section: Obligate Lytic Life Cyclementioning

confidence: 99%

Interaction of phages, bacteria, and the human immune system: Evolutionary changes in phage therapy

Jariah

Hakim

2019

Reviews in Medical Virology

View full text Add to dashboard Cite

Summary Phages and bacteria are known to undergo dynamic and co‐evolutionary arms race interactions in order to survive. Recent advances from in vitro and in vivo studies have improved our understanding of the complex interactions between phages, bacteria, and the human immune system. This insight is essential for the development of phage therapy to battle the growing problems of antibiotic resistance. It is also pivotal to prevent the development of phage‐resistance during the implementation of phage therapy in the clinic. In this review, we discuss recent progress of the interactions between phages, bacteria, and the human immune system and its clinical application for phage therapy. Proper phage therapy design will ideally produce large burst sizes, short latent periods, broad host ranges, and a low tendency to select resistance.

show abstract

“…The mef(A) gene is most frequently harboured by certain emm types such as emm1, emm2, emm3, emm4, emm9, emm12 and emm75, whereas emm types such as emm22, emm77, emm87 and emm89 have not yet been documented to carry any mef determinant (Creti et al 2005;Grivea et al 2006;Silva-Costa et al 2008;Wajima et al 2013). These observations suggest differences in the capacity of GAS emm types to acquire mef genes, and while the horizontal gene transfer of mef(A)-bearing genetic elements has been demonstrated in vitro by conjugation (Giovanetti et al 2003;Santagati et al 2003) and by transduction (Di Luca et al 2010), no comparison has yet been made of mef gene transfer frequencies among different GAS strains.…”

Section: Introductionmentioning

confidence: 97%

Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types

Hadjirin

Harrison

Holmes

et al. 2014

Letters in Applied Microbiology

View full text Add to dashboard Cite

The aim of this study was to examine the gene transfer potential of mef(A)-containing Tn120.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types. Using the filter mating technique, Tn1207.3 was transferred by conjugation to 23 macrolide-susceptible recipients representing 11 emm types. PCR analysis confirmed the presence of the mef(A) gene and the comEC junction regions of the Tn1207.3 insertion in resultant transconjugants. Significant variation was found in the transfer frequency of Tn1207.3 to different Strep. pyogenes strains, and this phenomenon may contribute to the differences in mef(A) frequency observed among clinical isolates.Significance and Impact of the StudyThe spread of antimicrobial resistance among pathogenic bacteria is an important problem, but the mechanisms of horizontal transfer between strains and species are often poorly understood. For instance, little is known on how macrolide resistance spreads between strains of the human pathogen Strep. pyogenes and why certain strains more commonly display resistance than others. Here, we show that Strep. pyogenes strains vary greatly in their ability to acquire a transposon encoding macrolide resistance by horizontal gene transfer in vitro. These data provide a novel insight into the transfer of antibiotic resistance between bacterial strains and offer an explanation for the differences in the frequency of resistance determinates and resistance seen among clinical isolates.Significance and Impact of the Study:The spread of antimicrobial resistance among pathogenic bacteria is an important problem, but the mechanisms of horizontal transfer between strains and species are often poorly understood. For instance, little is known on how macrolide resistance spreads between strains of the human pathogen Strep. pyogenes and why certain strains more commonly display resistance than others. Here, we show that Strep. pyogenes strains vary greatly in their ability to acquire a transposon encoding macrolide resistance by horizontal gene transfer in vitro. These data provide a novel insight into the transfer of antibiotic resistance between bacterial strains and offer an explanation for the differences in the frequency of resistance determinates and resistance seen among clinical isolates.

show abstract

Lysogenic Transfer of mef (A) and tet (O) Genes Carried by Φm46.1 among Group A Streptococci

Cited by 19 publications

References 22 publications

Acquired Inducible Antimicrobial Resistance in Gram-Positive Bacteria

Acquired Inducible Antimicrobial Resistance in Gram-Positive Bacteria

Interaction of phages, bacteria, and the human immune system: Evolutionary changes in phage therapy

Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types

Contact Info

Product

Resources

About