Lysobactin, a novel antibacterial agent produced by Lysobacter sp. I. Taxonomy, isolation and partial characterization.

O'Sullivan, J; McCullough, John E.; Tymiak, Adrienne A.; Kirsch, Donald R.; Trejo, William H.; Principe, Pacifico A.

doi:10.7164/antibiotics.41.1740

Cited by 85 publications

(62 citation statements)

References 10 publications

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“…The cell walls of hyphae and membranes of Peronosporomycetes zoospores are composed of ␤-1,3-and ␤-1,6-glucans and cellulose. Lysobacter species are known to produce several antibiotics (2,8,9,25,28) and antimicrobial enzymes, including ␤-1,3-glucanase (26,39,40). Therefore, our results which demonstrated that there was A. cochlioides zoospore lysis and hyphal necrosis attributable to SB-K88 antibiotic activity do not exclude a possible contribution by lytic enzymes acting in concert with xanthobaccin A.…”

Section: Discussionmentioning

confidence: 51%

Suppression of Damping-Off Disease in Host Plants by the Rhizoplane Bacterium Lysobacter sp. Strain SB-K88 Is Linked to Plant Colonization and Antibiosis against Soilborne Peronosporomycetes

Islam

Hashidoko

Deora

et al. 2005

Appl Environ Microbiol

207

130

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We previously demonstrated that xanthobaccin A from the rhizoplane bacterium Lysobacter sp. strain SB-K88 suppresses damping-off disease caused by Pythium sp. in sugar beet. In this study we focused on modes of Lysobacter sp. strain SB-K88 root colonization and antibiosis of the bacterium against Aphanomyces cochlioides, a pathogen of damping-off disease. Scanning electron microscopic analysis of 2-week-old sugar beet seedlings from seeds previously inoculated with SB-K88 revealed dense colonization on the root surfaces and a characteristic perpendicular pattern of Lysobacter colonization possibly generated via development of polar, brush-like fimbriae. In colonized regions a semitransparent film apparently enveloping the root and microcolonies were observed on the root surface. This Lysobacter strain also efficiently colonized the roots of several plants, including spinach, tomato, Arabidopsis thaliana, and Amaranthus gangeticus. Plants grown from both sugar beet and spinach seeds that were previously treated with Lysobacter sp. strain SB-K88 displayed significant resistance to the damping-off disease triggered by A. cochlioides. Interestingly, zoospores of A. cochlioides became immotile within 1 min after exposure to a SB-K88 cell suspension, a cell-free supernatant of SB-K88, or pure xanthobaccin A (MIC, 0.01 g/ml). In all cases, lysis followed within 30 min in the presence of the inhibiting factor(s). Our data indicate that Lysobacter sp. strain SB-K88 has a direct inhibitory effect on A. cochlioides, suppressing damping-off disease. Furthermore, this inhibitory effect of Lysobacter sp. strain SB-K88 is likely due to a combination of antibiosis and characteristic biofilm formation at the rhizoplane of the host plant.

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Section: Discussionmentioning

confidence: 51%

Suppression of Damping-Off Disease in Host Plants by the Rhizoplane Bacterium Lysobacter sp. Strain SB-K88 Is Linked to Plant Colonization and Antibiosis against Soilborne Peronosporomycetes

Islam

Hashidoko

Deora

et al. 2005

Appl Environ Microbiol

207

130

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“…Lysobacter is fast-growing, simple to use and maintain, and readily accessible to genetic tools, with capacities for posttranslational modifications and availability of the substrate pool for polyketides and nonribosomal peptide biosynthesis. Furthermore, Lysobacter is known as a prolific producer of bioactive compounds, such as cephabacins (11,28), HSAF (33,52), lysobactin (5,7,20,37), and tripropeptins (18,19). So far, none of these pathways has been genetically manipulated for new antibiotic production.…”

Section: Discussionmentioning

confidence: 99%

“…Lysobactin (katanosin B) was isolated from Lysobacter sp. SC14067 (7,20,37); tripropeptins were isolated from Lysobacter sp. BMK-333-48F3 (18,19).…”

mentioning

confidence: 99%

Identification and Characterization of the Anti-Methicillin-Resistant Staphylococcus aureus WAP-8294A2 Biosynthetic Gene Cluster from Lysobacter enzymogenes OH11

Zhang

Qian

et al. 2011

Antimicrob Agents Chemother

154

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Lysobactor enzymogenes strain OH11 is an emerging biological control agent of fungal and bacterial diseases. We recently completed its genome sequence and found it contains a large number of gene clusters putatively responsible for the biosynthesis of nonribosomal peptides and polyketides, including the previously identified antifungal dihydromaltophilin (HSAF). One of the gene clusters contains two huge open reading frames, together encoding 12 modules of nonribosomal peptide synthetases (NRPS). Gene disruption of one of the NRPS led to the disappearance of a metabolite produced in the wild type and the elimination of its antibacterial activity. The metabolite and antibacterial activity were also affected by the disruption of some of the flanking genes. We subsequently isolated this metabolite and subjected it to spectroscopic analysis. The mass spectrometry and nuclear magnetic resonance data showed that its chemical structure is identical to WAP-8294A2, a cyclic lipodepsipeptide with potent antimethicillin-resistant Staphylococcus aureus (MRSA) activity and currently in phase I/II clinical trials. The WAP-8294A2 biosynthetic genes had not been described previously. So far, the Gram-positive Streptomyces have been the primary source of anti-infectives. Lysobacter are Gram-negative soil/water bacteria that are genetically amendable and have not been well exploited. The WAP-8294A2 synthetase represents one of the largest NRPS complexes, consisting of 45 functional domains. The identification of these genes sets the foundation for the study of the WAP-8294A2 biosynthetic mechanism and opens the door for producing new anti-MRSA antibiotics through biosynthetic engineering in this new source of Lysobacter.

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“…Schlüsselschritte zum Abschluss der Totalsynthese von Kinamycin C . [106] Totalsynthesen [124] Hunderte semisynthetischer Analoga von Ramoplanin A2 sind bereits untersucht worden, [115] [126] und von Shoji et al bei Shionogi beschrieben wurde. [127] Es ist hochaktiv gegen Gram-positive Bakterien (beispielsweise MIC = 0.06 mg mL À1 gegen Streptococcus pneumoniae; MIC(Vancomycin) = 0.5 mg mL À1 ) und zeigt auch Aktivität gegen Stämme, die gegen viele andere Antibiotika (unter anderem Vancomycin) resistent sind.…”

Section: Ramoplanin A2unclassified

Fortschritte in der Chemie und Biologie natürlicher Antibiotika

et al. 2009

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Seit der bahnbrechenden Entdeckung von Penicillin wurde die Vielfalt natürlich vorkommender Moleküle ausgiebig hinsichtlich ihrer Eignung als Medikamente sowie zum Auffinden neuer Leitstrukturen beim Wirkstoffdesign untersucht. Die Suche nach Wirkstoffen, mit denen Infektionskrankheiten bekämpft werden können, war dabei von besonderem Interesse und verlief sehr erfolgreich. Die Erforschung der Antibiotika weist eindrucksvolle Entdeckungen und Geschichten über die Entwicklung von Wirkstoffen auf, von denen die große Mehrheit ihren Ursprung in Naturstoffen hat. Die Chemie und besonders die chemische Synthese haben eine bedeutende Rolle dabei gespielt, natürlich vorkommende Antibiotika und deren Derivate für die medizinische Anwendung bereitzustellen, und zweifellos werden diese Disziplinen auch künftig Schlüsseltechnologien sein. In dieser Übersicht stellen wir einige der bedeutendsten neueren Entwicklungen und Fortschritte in der Chemie, Biologie und Medizin natürlich vorkommender Antibiotika vor, wobei die Totalsynthese, das Design von Analoga und die biologische Bewertung von Molekülen mit neuartigen Wirkmechanismen im Vordergrund stehen.

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Lysobactin, a novel antibacterial agent produced by Lysobacter sp. I. Taxonomy, isolation and partial characterization.

Cited by 85 publications

References 10 publications

Suppression of Damping-Off Disease in Host Plants by the Rhizoplane Bacterium Lysobacter sp. Strain SB-K88 Is Linked to Plant Colonization and Antibiosis against Soilborne Peronosporomycetes

Suppression of Damping-Off Disease in Host Plants by the Rhizoplane Bacterium Lysobacter sp. Strain SB-K88 Is Linked to Plant Colonization and Antibiosis against Soilborne Peronosporomycetes

Identification and Characterization of the Anti-Methicillin-Resistant Staphylococcus aureus WAP-8294A2 Biosynthetic Gene Cluster from Lysobacter enzymogenes OH11

Fortschritte in der Chemie und Biologie natürlicher Antibiotika

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