Lysobacter selenitireducens sp. nov., isolated from river sediment

Abstract

A Gram-stain-negative, yellow-pigmented, motile, flagellated and rod-shaped bacterium, designated as 13AT, was isolated from a river sediment sample of Fuyang River in Hengshui City, Hebei Province, PR China. Strain 13AT grew at 10–37 °C (optimum, 30 °C), at pH 5.0–11.0 (optimum, pH 7.0) and at 0–7 % (w/v) NaCl concentration (optimum, 0 %). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain 13AT belongs to the genus Lysobacter , and was most closely related to Lysobacter spongiicola DSM 21749T (97.8 %), Lysobacter concretionis DSM 16239T (97.5 %), Lysobacter daejeonensis GIM 1.690T (97.3 %) and Lysobacter arseniciresistens CGMCC 1.10752T (96.9 %). Meanwhile, the type species Lysobacter enzymogenes ATCC 29487T was selected as a reference strain (95.2 %). The genomic size of strain 13AT was 3.0 Mb and the DNA G+C content was 69.0 %. The average nucleotide identity values between strain 13AT and each of the reference type strains L. spongiicola DSM 21749T, L. concretionis DSM 16239T, L. daejeonensis GIM 1.690T, L. arseniciresistens CGMCC 1.10752T and L. enzymogenes ATCC 29487T were 75.9, 76.1, 77.7, 78.0 and 73.2 %, respectively. The digital DNA–DNA hybridization values between strain 13AT and each of the reference type strains were 21.7, 22.2, 21.9, 22.7 and 23.2 %, respectively. The average amino acid identity values between strain 13AT and each of the reference type strains were 72.5, 72.9, 72.3, 75.0 and 69.2 %, respectively. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). The sole respiratory quinone was identified as ubiquinone-8. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified lipid, four unidentified phospholipids and two unidentified glycolipids. Based on the phenotypic, physiological, phylogenetic and chemotaxonomic data, strain 13AT represents a novel species of the genus Lysobacter , for which the name Lysobacter selenitireducens sp. nov. is proposed. The type strain is 13AT (=JCM 34786T=GDMCC 1.2722T).

Genetic Basis and Expression Pattern Indicate the Biocontrol Potential and Soil Adaption of Lysobacter capsici CK09

“…Since then, ANI analysis has been widely utilized as a reliable tool for taxonomic reclassification [ 63 , 64 ]. Recently, ANI analysis has been applied in the identification of novel species in Lysobacter [ 65 , 66 ]. In a previous study, L. capsici CK09 was recognized based on the 16S rRNA gene [ 35 ].…”

Lysobacter stagni sp. nov. and Limnohabitans lacus sp. nov., isolated from a pond

“…Lysobacter species share some common features. For instance, they are Gram-negative, rod-shaped, and aerobic bacteria [3,4]. The major fatty acids are iso-C 15 : 0 , iso-C 16 : 0 , and summed feature 9 (iso-C 17 : 1 ω9c and/or C 16 : 0 10-methyl), and the major polar lipids are phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol [3].…”