Lysis of yeast cells and erythrocytes by dimethylsulfoxide

Bruijne, Adriaan W. De; Steveninck, J. Van

doi:10.1016/0006-2952(72)90265-1

Cited by 13 publications

(4 citation statements)

References 27 publications

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“…Osmotic clamp was induced by adding 2% (vol) of the osmolyte, DMSO, to a split sample of yeast cells in aqueous suspension. This low concentration of DMSO has been shown to significantly inhibit osmotic equilibration due to its high osmolarity and rapid transport rate, while not significantly affecting the metabolic state of the cells. , Both the control cells (without DMSO) and cells under osmotic clamp were deposited onto weakly condensed lipid-templated silica films and allowed to age for 1 or 2 h, after which the substrates were rinsed thoroughly to remove non-integrated material. The percentage of retained cells under both conditions is presented in Figure A.…”

Section: Resultsmentioning

confidence: 99%

Cell-Directed Integration into Three-Dimensional Lipid−Silica Nanostructured Matrices

Harper

Khirpin²,

Carnes³

et al. 2010

ACS Nano

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We report a unique approach in which living cells direct their integration into 3D solid-state nanostructures. Yeast cells deposited on a weakly condensed lipid/silica thin film mesophase actively reconstruct the surface to create a fully 3D bio/nano interface, composed of localized lipid bilayers enveloped by a lipid/silica mesophase, through a self-catalyzed silica condensation process. Remarkably, this integration process selects exclusively for living cells over the corresponding apoptotic cells (those undergoing programmed cell death), via the development of a pH gradient, which catalyzes silica deposition and the formation of a coherent interface between the cell and surrounding silica matrix. Added long-chain lipids or auxiliary nanocomponents are localized within the pH gradient, allowing the development of complex active and accessible bio/nano interfaces not achievable by other synthetic methods. Overall, this approach provides the first demonstration of active cell-directed integration into a nominally solid-state three-dimensional architecture. It promises a new means to integrate "bio" with "nano" into platforms useful to study and manipulate cellular behavior at the individual cell level and to interface living organisms with electronics, photonics, and fluidics.

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Section: Resultsmentioning

confidence: 99%

Cell-Directed Integration into Three-Dimensional Lipid−Silica Nanostructured Matrices

Harper

Khirpin²,

Carnes³

et al. 2010

ACS Nano

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“…The effect of the former on brain potassium has been noted by others s, and has been felt to be the consequence of the "sealing" effect on membranes by pentobarbitone 1. Conversely, DMSO has been left to create an extracellular "leakage" of potassium, and consequently it increases the total brain potassium 10 We conclude that DMSO offers certain advantages for the control of intracranial hypertension and brain oedema, and further work as to its efficacy and side effects should be performed.…”

Section: Electrolyte Changes In the Hemispherementioning

confidence: 88%

Comparison of the effects of DMSO and pentobarbitone on experimental brain oedema

et al. 1982

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The purpose of this report is to compare the effect of acute therapy with dimethyl sulphoxide (DMSO) and pentobarbitone on experimental brain oedema produced by a cryogenic lesion over the left hemisphere in albino rabbits. A group of animals received DMSO (1 mg/kg-10% solution) by intravenous bolus, and another group received a pentobarbitone intravenous 30-minute infusion (40 mg/kg). Intracranial pressure (ICP), systolic arterial pressure (SAP), central venous pressure (CVP), and EEG were studied. Brain water and electrolyte content were analyzed at one hour following the initiation of therapy. ICP was promptly reduced with both forms of therapy. A 66% reduction from control values was reached at 50 +/- 12 minutes with pentobarbitone, and a 45% reduction from control values was reached at 30 minutes with DMSO. There was no significant reduction in the water content of the brain with either form of therapy. A significant elevation in brain potassium content was noted following DMSO when compared to untreated controls. CVP was essentially unchanged in both groups. Pentobarbitone produced a reduction of SAP with a mean value of 20.3 torr at 45 minutes from infusion. DMSO produced no reduction of SAP. It is concluded that DMSO and pentobarbitone are just as effective in reducing ICP. DMSO has the capacity to maintain SAP which pentobarbitone does not have, thus assuring a better cerebral perfusion pressure.

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“…There are reports (5,25) in which the effects of DMSO on S. cerevisiae cells were examined, but the concentration of DMSO that was used was much higher than that employed here. Results of one report (25) indicated that DMSO induces the formation of respiratory-deficient petite mutants in S. cerevisiae at concentrations higher than 8% in the growth medium.…”

Section: Discussionmentioning

confidence: 99%

Induction and Characterization of Artificial Diploids from the Haploid Yeast Torulaspora delbrueckii

Sasaki

Ohshima

1987

Appl Environ Microbiol

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The yeast Torulaspora delbrueckii, which propagates as a haploid, was made into a diploid by treatment with dimethyl sulfoxide (DMSO) on the regeneration of protoplasts. The diploid state was stably inherited; the cell volume was three times that of the parent strain and the cellular DNA content was two times that of the parental strain. No essential difference was found between diploids induced by DMSO and those formed through intraspecific protoplast fusion. The diploid strains sporulated fairly well, with their cells converting directly into asci. Random spore analysis revealed that diploids induced through protoplast fusion gave rise to auxotrophic segregants (haploids) with the parental genetic marker or to segregants formed by recombination, while diploids induced by DMSO from a doubly auxotrophic parent gave rise to no recombinant, indicating that it was chromosomally homoallelic in nature. The magnesium level in the protoplast regeneration medium was found to be an important factor for inducing diploid formation. At 0.2 mM magnesium diploids appeared even in the absence of DMSO, while at 2 mM magnesium diploids never appeared unless DMSO was added to the regeneration medium. Evidence is provided that the diploids induced by DMSO or a low magnesium level are due to direct diploidization but not protoplast fusion. UV light irradiation of intact cells (without protoplasts), 10% of which survived, also produced diploids among this surviving population. From these results we conclude that the perturbation of protoplast regeneration or of cell division by the treatments mentioned above somehow induced direct diploidization of T. delbrueckii.

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Lysis of yeast cells and erythrocytes by dimethylsulfoxide

Cited by 13 publications

References 27 publications

Cell-Directed Integration into Three-Dimensional Lipid−Silica Nanostructured Matrices

Cell-Directed Integration into Three-Dimensional Lipid−Silica Nanostructured Matrices

Comparison of the effects of DMSO and pentobarbitone on experimental brain oedema

Induction and Characterization of Artificial Diploids from the Haploid Yeast Torulaspora delbrueckii

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