Lysis of mycobacteria-infected monocytes by IL-2-activated killer cells: Role of LFA-1

Blanchard, D K; Michelini-Norris, MB; Friedman, Herman; Djeu, Julie Y.

doi:10.1016/0008-8749(89)90254-2

Cited by 46 publications

(20 citation statements)

References 24 publications

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“…In contrast, the increased induction of IL-10 production might correlate with the virulence of the mycobacteria. For example, the virulent MTB strain H37Rv induces more IL-10 than the attenuated H37Ra, even though both strains induce production of similar amounts of TNF-a [7]. Thus, increased IL-10 production might be an adaptation of the pathogen to survive within phagocytes, although M. avium serovar 4 might not be fully adapted to completely abrogate the action of TNF-a.…”

Section: Discussionmentioning

confidence: 96%

Interleukin 10 Produced by Macrophages Inoculated withMycobacterium aviumAttenuates Mycobacteria‐Induced Apoptosis by Reduction of TNF‐α Activity

1999

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Normal human macrophages respond to infection with Mycobacterium avium, serovar 4, by producing tumor necrosis factor (TNF)-alpha, which mediates apoptosis, and by elaborating interleukin (IL)-10, a TNF-alpha antagonist. We show that IL-10 down-regulates apoptosis by inhibiting the TNF-alpha production of the inoculated macrophages and by inducing the release of soluble TNF receptor type 2 from the macrophages, which leads to inactivation of TNF-alpha. These experiments suggest that induction of IL-10 production is a virulence factor that creates an intracellular sanctuary for the bacteria that is inaccessible to the defense mechanisms of the host.

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Section: Discussionmentioning

confidence: 96%

Interleukin 10 Produced by Macrophages Inoculated withMycobacterium aviumAttenuates Mycobacteria‐Induced Apoptosis by Reduction of TNF‐α Activity

1999

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“…In particular, IFN-γ is widely used as an indicator for vaccine efficacy [43], whereas IL-2 is associated with memory T cells and plays an important role in the long-term survival [44,45]. Several studies have demonstrated that IL-2 can influence the course of Mtb infections, either alone or in combination with other cytokines [46,47]. In addition, IL-2 is also a growth factor of both CD4+ and CD8+ T cells.…”

Section: Discussionmentioning

confidence: 99%

The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

Gu¹,

Chen²,

Mi³

et al. 2016

ABBS

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Tuberculosis remains a major global health problem and effective vaccines are urgently needed. In this study, we used the combined DNA-and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccine may substantially enhance the immunogenicity of BCG and strengthen both CD4+ T cell-mediated Th1 and CD8+ T cell-mediated cytolytic responses. Compared with the protein-based vaccine, the DNA-based vaccine can induce more durable Th1 immune response, characterized by high levels of antibody response, proliferation response, percentages of CD4+/CD8+ and cytokine secretion in antigen-stimulated splenocyte cultures. In conclusion, we for the first time, developed a protein-and plasmid DNA-based booster vaccine based on Rv0577. Our findings suggest that antigen Rv0577-based DNA vaccine is immunogenic and can efficiently boost BCG, which could be helpful in the design of an efficient vaccination strategy against TB.

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“…More recent findings indicated that NK cells are cytotoxic in a nonrestricted manner and stimulate mycobacteriostatic and mycobactericidal activities in infected macrophages (50,55,270). The cytotoxicity of NK cells for infected macrophages appears to depend on binding via the LFA-1 glycoprotein receptor (56); however, the validity of this observation could be questioned since other recent studies showed that NK cells do not efficiently bind or lyse target cells expressing the class I major histocompatibility complex. It is of interest to note that Blanchard and colleagues (57) showed that NK cells exposed to M. avium release large amounts of IL-6, which may have an important influence on the host immune response.…”

Section: Role Of Nk Cellsmentioning

confidence: 99%

The Mycobacterium avium complex

1993

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Mycobacterium avium complex (MAC) disease emerged early in the epidemic of AIDS as one of the common opportunistic infections afflicting human immunodeficiency virus-infected patients. However, only over the past few years has a consensus developed about its significance to the morbidity and mortality of AIDS. M. avium was well known to mycobacteriologists decades before AIDS, and the MAC was known to cause disease, albeit uncommon, in humans and animals. The early interest in the MAC provided a basis for an explosion of studies over the past 10 years largely in response to the role of the MAC in AIDS opportunistic infection. Molecular techniques have been applied to the epidemiology of MAC disease as well as to a better understanding of the genetics of antimicrobial resistance. The interaction of the MAC with the immune system is complex, and putative MAC virulence factors appear to have a direct effect on the components of cellular immunity, including the regulation of cytokine expression and function. There now is compelling evidence that disseminated MAC disease in humans contributes to both a decrease in the quality of life and survival. Disseminated disease most commonly develops late in the course of AIDS as the CD4 cells are depleted below a critical threshold, but new therapies for prophylaxis and treatment offer considerable promise. These new therapeutic modalities are likely to be useful in the treatment of other forms of MAC disease in patients without AIDS. The laboratory diagnosis of MAC disease has focused on the detection of mycobacteria in the blood and tissues, and although the existing methods are largely adequate, there is need for improvement. Indeed, the successful treatment of MAC disease clearly will require an early and rapid detection of the MAC in clinical specimens long before the establishment of the characteristic overwhelming infection of bone marrow, liver, spleen, and other tissue. Also, a standard method of susceptibility testing is of increasing interest and importance as new effective antimicrobial agents are identified and evaluated. Antimicrobial resistance has already emerged as an important problem, and methods for circumventing resistance that use combination therapies are now being studied.

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Lysis of mycobacteria-infected monocytes by IL-2-activated killer cells: Role of LFA-1

Cited by 46 publications

References 24 publications

Interleukin 10 Produced by Macrophages Inoculated withMycobacterium aviumAttenuates Mycobacteria‐Induced Apoptosis by Reduction of TNF‐α Activity

Interleukin 10 Produced by Macrophages Inoculated withMycobacterium aviumAttenuates Mycobacteria‐Induced Apoptosis by Reduction of TNF‐α Activity

The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

The Mycobacterium avium complex

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Lysis of mycobacteria-infected monocytes by IL-2-activated killer cells: Role of LFA-1

Cited by 46 publications

References 24 publications

Interleukin 10 Produced by Macrophages Inoculated withMycobacterium aviumAttenuates Mycobacteria‐Induced Apoptosis by Reduction of TNF‐α Activity

Interleukin 10 Produced by Macrophages Inoculated withMycobacterium aviumAttenuates Mycobacteria‐Induced Apoptosis by Reduction of TNF‐α Activity

The &lt;italic&gt;Mycobacterium bovis&lt;/italic&gt; BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

The Mycobacterium avium complex

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The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice