Lysis of Halobacteria with Bile Acids and Proteolytic Enzymes of Halophilic Archaeobacteria

The Cell Surface Glycoprotein ofHaloarcula japonicaTR-1

“…Halophilic archae bacteria showed different susceptibility to proteolysis, suggesting that individual strains had CSG with different moieties. 9 ) In this paper, we demonstrate the occurrence of similar glycoprotein on the cell surface of Ha. japonica TR-1.…”

Halophilic Microorganisms and their Environments

“…Exposure to bacitracin, which interferes with the glycosylation of the protein, caused the formation of spherical cells, thus supplying further documentation for the structural, shape-maintaining role of the Halobacterium salinarum glycoprotein (Mescher and Strominger, 1976b). Bile acids in low concentrations have a destabilizing effect on the S-layer cell wall of many archaeal halophiles (Dussault, 1956a;Kamekura and Seno, 1991;, thereby providing a simple diagnostic test to obtain information on the type of cell wall present (Dussault, 1956b). Loss of the native shape with the formation of spherical structures is also induced when the pH is lowered to values below 4 (Kushner and Bayley, 1963).…”

Measurement of in situ halophilic glyceraldehyde-3-phosphate dehydrogenase activity from the permeabilized cells of archaebacterium Haloarcula vallismortis.

“…Earlier studies from our laboratory have shown the possibility of permeabilizing Gram-negative cells using lysozyme (D'Souza et al, 1982). Proteolytic studies have previously shown varied responses by different strains suggesting a difference in cell surface glycoprotein moieties (Kamekura and Seno, 1991). In this study, H. vallismortis showed effective cell permeabilization with papain.…”