Lysis of Collagen by Human Gingival Bacteria.

Schultz-Haudt, Stig D.; Scherp, Henry W.

doi:10.3181/00379727-89-21920

Cited by 26 publications

(8 citation statements)

References 2 publications

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“…Regarding the etiology of periodontal disease in man, one possibility is that products of bacteria present in dental plaque diffuse across the gingival epithelium and directly induce inflammation and tissue damage, including bone resorption (26)(27)(28). Endotoxin could be such a product of growing bacteria.…”

mentioning

confidence: 99%

Role of bacterial products in periodontitis: immune response in gnotobiotic rats monoinfected with Eikenella corrodens

Johnson¹,

Behling²,

Lai³

et al. 1978

Infect Immun

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The development of humoral and cell-mediated immune responses to Eikenella corrodens (a bacterium that causes periodontal lesions in gnotobiotic rats) was measured and compared with the rate of appearance of macroscopic lesions. A possible inverse relationship was found. A strong cell-mediated immune response, as measured by skin reactivity and lymphocyte mitogenesis, occurred between 4 and 6 weeks after infection and subsided soon thereafter to a low response level. Humoral antibodies to endotoxin from E. corrodens could not be detected at any time. The disease developed only after the cell-mediated immune response diminished, thus suggesting that lack of an efficient immune response may permit the development of the disease. This is seemingly in contradiction to the assumption that tissue destruction in such cases is caused by the immune response and its products. We are inclined to believe, based on our findings reported here, that the lack of immune responsiveness to the bacterium and/or its products is the major causative factor in the development of periodontitis. At the same time, we wish to emphasize that occurrence of both phenomena during the long development of periodontal disease is possible.

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mentioning

confidence: 99%

Role of bacterial products in periodontitis: immune response in gnotobiotic rats monoinfected with Eikenella corrodens

Johnson¹,

Behling²,

Lai³

et al. 1978

Infect Immun

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“…It is well known that certain Clostridiuni species produce proteolytic enzymes capable of hydrolyzing native collagen, but apparently other bacteria possessing such enzymes have not been reported. Organisms with this capacity have been sought unsuccessfully in traumatic wounds (MacLennen, Mandl, and Howes, 1953), among salt tolerant bacteria (Everett and Cordon 1955), and in the oral cavity of man (Roth and Meyers, 1956;Schultz-Haudt and Scherp, 1955;Mergenhagen and Scherp, 1960). It has seemed likely that collagenolytic organisms are among the members of the oral microbiota since collagen degradation is a feature of both dentinal caries (Engel, 1950) and periodontal disease (Lucas and Thonard, 1955).…”

mentioning

confidence: 99%

DEGRADATION OF COLLAGENOUS SUBSTRATES BY BACTEROIDES MELANINOGENICUS

Gibbons¹,

Macdonald²

1961

J Bacteriol

177

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“…(Mayrand et al 1980, Aifano et al 1974, Coiiagenoiytic activity by orai microor -Soderling & Paunio 1981). More recently, ganisms, which hydroiyzed native coiiagen, Robertson et al (1982) have observed coilavi'as first described by Schuitz-Haudt and genoiytic activity in both media and ceil Scherp (1955) in mixed culture of gingival sonicate supernatants of some strains of Bacteroides species and Aetinobacillus actinomycetemcomitans. The coliagenoiytic activity in these studies was inhibited or at least unaffected by reducing reagents having thiol-groups.…”

Section: Introductionmentioning

confidence: 88%

Thiol‐dependent collagenolytic activity in culture media of Bacteroides gingivalis

Toda

Otsuka

Ishikawa

et al. 1984

J of Periodontal Research

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Twenty strains of anaerobic "bacteria including some oral species from Bacteroides, Eikenelia, Eusobacterium, Capnocytophaga, and Actinobacillus were used for the detection of collagenolytic activity in their culture media (supernatants). Organisms were essentially grown in complete anaerobic basal broth, and coliagenolytic activities in the culture media were measured using '^C-acetylated soluble collagen from rat skin. When the enzyme reaction was carried out in the standard reaction mixture for 60 min, very low collagenolytic activities were found in culture media of Bacteroides gingivalis 381, 1021, Bacteroides intermedius 163, Kl-8, Bacteroides asaccharolyticus ATCC 27067, and Eusobacterium nucteatum ATCC 25586. However, the activity in the media of all species of B. gingivalis tested was enormously enhanced by the addition of reducing reagents having thiol-groups such as dithiothreitol, L-cysteine, etc. This stimulated activity was nearly completely inhibited by some thiol-blocking reagents. In contrast, these reagents had no effect on the collagenolytic activity in the media of the other anaerobes and in the coUagenase preparation of Clostridium histolyticum. These results suggest that B. gingivalis produces thiol-dependent collagenolytic enzyme which may play an important role in the degradation of coilagen fibrils in a reduced environment like the deep gingival pocket.

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Lysis of Collagen by Human Gingival Bacteria.

Cited by 26 publications

References 2 publications

Role of bacterial products in periodontitis: immune response in gnotobiotic rats monoinfected with Eikenella corrodens

Role of bacterial products in periodontitis: immune response in gnotobiotic rats monoinfected with Eikenella corrodens

DEGRADATION OF COLLAGENOUS SUBSTRATES BY BACTEROIDES MELANINOGENICUS

Thiol‐dependent collagenolytic activity in culture media of Bacteroides gingivalis

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