Lysis and viability of cultured mammalian cells exposed to 1 MHz ultrasound

Kaufman, Gary E.; Miller, Morton W.; Griffiths, Tony; Ciaravino, Victor; Carstensen, Edwin L.

doi:10.1016/0301-5629(77)90117-x

Cited by 56 publications

(14 citation statements)

References 12 publications

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“…In 1989, Miller et al [321 ] came up with a similar shielding theory to explain the observation, noted by several workers [311,[322][323][324][325] that, in the insonation of a rotating sample, as the ultrasonic intensity was increased the effect (cell lysis) firstly increased, then decreased as the intensity was further increased.…”

mentioning

confidence: 85%

Effects and Mechanisms

Leighton

1994

The Acoustic Bubble

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mentioning

confidence: 85%

Effects and Mechanisms

Leighton

1994

The Acoustic Bubble

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“…Follicles filled with colloids were seen to be closely packed and surrounded by rich capillary network. The normalcy can be associated to the fact that the ultrasound heating is not focused directly on the foetal thyroid gland as ultrasound at sufficiently high intensities can generate cavitational activity that completely destroys cells (Kaufman et al, 1977;Moore & Coakley, 1977). Also, high intensity focused ultrasound (HIFU) have been reportedly used to treat patients with thyroidal nodes; exposed tissues became necrotic that later disintegrated and later shrank (Huedayi et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effect of multiple in-utero insonation on rabbit fetal thyroid hormonal level

Idris¹,

Audu²,

Fadason³

et al. 2017

Sokoto J. Vet. Sc.

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Due to the increased use of ultrasound in the diagnosis of cyesis, it is necessary to study the possible adverse effect of insonation on thyroid hormone in fetuses. Sixteen (16) adult rabbit does that had kindled at least once were used for the study and were scanned using a Medison S600V ® Ultrasound machine with a 6.5MHz transcutaneous curve-linear probe. All does were mated naturally by the introduction of a doe to a buck and then divided randomly into two groups. The does that were scanned (insonated)-, were properly restrained on dorsal recumbency, liberally shaved; aquasonic gel applied and scanned using a transcutaneous curve-linear probe for 5 minutes on the 5 th day post coitus and thereafter on days 7, 12, 15, 20, 25, 27 and 29. The restrain and scanning procedures were mimicked on the group of does that were not scanned (non-insonated). Upon parturition, blood was collected from kits and serum was extracted and used to assay for total thyroxine (tT4) and triiodothyronine (tT3). Fetal thyroid glands were also harvested for histologic study. Mean tT3 concentration was 1.60 ± 0.90 µg/dl and 1.58 ± 0.03 µg/dl while mean tT4 was 1.46 ± 0.10 µg/dl and 1.39 ± 0.07 µg/dl for the non-insonated and insonated groups, respectively. P values of 0.610 and 0.132 for tT3 and tT4 concentration respectively showed that there is no statistically significant difference in the values even though there was a numerical difference between the groups. No histopathologic change on fetal thyroid gland from both groups was observed. In conclusion, although the values were not significantly different, the numerical decrease of both tT3 and tT4 in the insonated rabbits suggest the possibility that insonation could alter their values in fetuses when the threshold of ultrasound waves are increased and insonation prolonged.

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“…Ultrasound may damage cells irreversibly and induce severe cell membrane modifications (2)(3)(4). The effect of ultrasound on cells has been correlated with the generation of acoustic cavitations (2,5,6). Several studies have suggested that cavitations resulting from the collapse of gas bubbles generated by acoustic pressure fields may be the cause of cell damage following ultrasonic irradiation (7,8).…”

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

Zhang

Zhou

Cheng

et al. 2014

Molecular Medicine Reports

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The present study aimed to investigate whether low frequency low energy ultrasound combined with microbubbles induces apoptotic cell death of A7r5 rat aortic vascular smooth muscle cells, and to identify the possible mechanisms underlying this effect. Ultrasonic waves (45 kHz with 0.3 Wcm2 of intensity for 0, 10, 20 and 30 sec) were used together with different dosages of SonoVue™ microbubbles (0, 14, 28, 42 and 56 µl), respectively. The cell viability and apoptotic rate were determined by trypan blue staining immediately following treatment and flow cytometry 24 h thereafter. The treatment conditions resulting in the lowest amount of necrosis, highest apoptotic rate and lowest microbubble dosage was selected for the US+MB group, which was treated with ultrasound combined with microbubbles. The cell proliferation 24 h following treatment was determined and western blot analysis was applied to examine the expression of apoptosis‑associated proteins, B-cell lymphoma 2 (Bcl‑2) and Bcl-2-associated X (Bax). The harmonic acoustic pressure amplitude was measured to obtain the cavitation intensity. The combination of 20 sec ultrasound irradiation and 14 µl SonoVue™ was selected as the treatment conditions for the US+MB group. The results demonstrated that both ultrasound alone (the US group) and in combination with microbubbles significantly inhibited the proliferation of A7r5 cells compared with that of the control (P<0.01), and the suppression in the US+MB group was significantly greater (P<0.01). The apoptotic rate in A7r5 cells induced by this combination treatment (16.62±0.93%) was significantly higher than that in the control (3.93±0.39%; P<0.01) and US (6.88±1.87%; P<0.01) groups. Treatment with ultrasound combined with microbubbles increased the expression of Bax and decreased the ratio of Bcl‑2/Bax compared with those in the control and US groups. The cavitation induced by ultrasound combined with microbubble treatment was more intense than that by ultrasound alone. The results demonstrated that the cell death and apoptosis of A7r5 cells were associated with ultrasound duration and microbubble dosage. Low frequency ultrasound combined with microbubbles induced apoptosis in A7r5 cells through the upregulation of Bax and the downregulation of the Bcl‑2/Bax ratio, where the cavitation effect may have an important role.

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Lysis and viability of cultured mammalian cells exposed to 1 MHz ultrasound

Cited by 56 publications

References 12 publications

Effects and Mechanisms

Effects and Mechanisms

Effect of multiple in-utero insonation on rabbit fetal thyroid hormonal level

Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

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