Lysinibacillus sp. GG242 from Cattle Slurries Degrades 17β-Estradiol and Possible 2 Transformation Routes

Abstract

Environmental estrogen pollution has long been a concern due to adverse effects on organisms and ecosystems. Biodegradation is a vital way to remove estrogen, a strain of Lysinibacillus sp. was isolated, numbered strain GG242. The degradation rate of 100 mg·L−1 17β-estradiol (E2)) > 95% in one week, and compared with extracellular enzymes, intracellular enzymes have stronger degradation ability. Strain GG242 can maintain a stable E2 degradation ability under different conditions (20–35 °C, pH 5–11, salinity 0–40 g·L−1). Under appropriate conditions (30 °C, pH 8, 1 g·L−1 NaCl), the degradation rate increased by 32.32% in one week. Based on the analysis of transformation products, inferred E2 was converted via two distinct routes. Together, this research indicates the degradation potential of strain GG242 and provides new insights into the biotransformation of E2.

Biodegradation of 17 β-estradiol by Serratia marcescens and Stenotrophomonas tumulicola co-culture isolated from a sewage treatment plant in Upper Egypt

“…We found that 93.6% of E2 was degraded at pH 7.00, while Liu et al and AlAhadeb found that 90% and 91.3% of E2 were broken down at pH 7.00 and pH 7.50, respectively ( 33 ) and ( 32 ). In contrast, Wu et al used pH 5.00 and 11.00 to achieve a 90% degradation rate ( 34 ).…”

Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

“…This is the most potent form of estrogen, and its concentration is highest in females of childbearing age. Some physicians prescribe it as a marker for ovary health [ 9 , 10 ]. The levels of 17β-estradiol in rivers and freshwater have been reported by wastewater treatment plants (WWTPs) in North America to be 1–22 ng/L and 0–4.5 ng/L, respectively [ 11 ].…”