Lysine Succinylation and Acetylation in <i>Pseudomonas aeruginosa</i>

Gaviard, Charlotte; Broutin, Isabelle; Cosette, Pascal; Dé, Emmanuelle; Jouenne, Thierry; Hardouin, Julie

doi:10.1021/acs.jproteome.8b00210

Cited by 66 publications

(85 citation statements)

References 78 publications

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“…Most notably, Gingipain R1 (B2RM93) harbors 11 Ksuc modification sites. The distribution of the number of Ksuc sites per protein is different from that found in E coli , M tuberculosis , P aeruginosa , and V parahaemolyticus , all of which have only one Ksuc site in 40%‐50% of identified proteins …”

Section: Resultscontrasting

confidence: 66%

“…All 233 identified Ksuc proteins account for 11.1% of the total proteins in P gingivalis ATCC 33277. The percentage of Ksuc proteins to total proteins is similar to that identified in other bacterial species, including Escherichia coli , Mycobacterium tuberculosis , Pseudomonas aeruginosa , and Vibrio parahemolyticus . Of the 233 identified Ksuc proteins, approximately 75% (174) possess a single Ksuc site and 11.6% (27) contain 3 or more Ksuc sites.…”

Section: Resultssupporting

confidence: 65%

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Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Gong

Zhou

et al. 2019

Molecular Oral Microbiology

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The gram-negative anaerobe Porphyromonas gingivalis is not only a keystone periodontal pathogen but also an emerging systemic pathogen. Although the newly discovered protein post-translational modification (PTM), lysine succinylation (Ksuc), appears to play an important role in modulating metabolic processes in bacteria, this PTM has not been investigated in P gingivalis. In this study, we used a highly sensitive proteomics approach combining affinity enrichment with high-resolution liquid chromatography coupled with tandem mass spectrometry to examine Ksuc in P gingivalis.In total, 345 Ksuc sites in 233 proteins were identified and determined to be involved in a variety of cellular processes. In the region surrounding Ksuc sites, lysine residues were drastically overrepresented and sequence motifs with succinyl-lysine flanked by a lysine at the +3 or +6 positions appear to be unique to this pathogen. Additionally, our results suggest a crosstalk between Ksuc and glycosylation, but the overlap between Ksuc and acetylation in P gingivalis is quite different from that observed in other organisms. Notably, Ksuc was observed in proteins associated with established virulence factors, including gingipains, fimbriae, RagB, and PorR. Moreover, products of the factors necessary for P gingivalis in vitro survival (18.5%) were found to be succinylated at lysine sites and the same was observed in products of fitness factors for P gingivalis survival in both abscess and epithelial cell colonization environments (12%). Collectively, these results suggest that Ksuc may be a new mechanism in modulating the virulence, adaptation, and fitness of P gingivalis. K E Y W O R D S fitness factors, lysine succinylation, necessary factors, Porphyromonas gingivalis, proteomics | 75 WU et al.

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Section: Resultscontrasting

confidence: 66%

Section: Resultssupporting

confidence: 65%

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Gong

Zhou

et al. 2019

Molecular Oral Microbiology

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“…Several proteins involved in oxidative stress response and antibiotic sensitivity exhibited a modified expression level. The abundance of three proteins (PA14_18690, OpdB, and OprG) were increased and seven others (PA14_47550, PA14_18330/50, ObgE, MexG, MexH, and OmpD) were reduced with both treatments as compared to the control (Aendekerk, 2005;Breidenstein et al, 2008;Dotsch et al, 2009;Balasubramanian et al, 2013;Verstraeten et al, 2015;Sakhtah et al, 2016;Chevalier et al, 2017;Gaviard et al, 2018;Wolloscheck et al, 2018). Several other protein levels showed differences between the two lactonase treatments.…”

Section: Treatment Of Pa14 With Different Lactonases Leads To Distincmentioning

confidence: 90%

Lactonase Specificity Is Key to Quorum Quenching in Pseudomonas aeruginosa

et al. 2020

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The human opportunistic pathogen Pseudomonas aeruginosa orchestrates the expression of many genes in a cell density-dependent manner by using quorum sensing (QS). Two acyl-homoserine lactones (AHLs) are involved in QS circuits and contribute to the regulation of virulence factors production, biofilm formation, and antimicrobial sensitivity. Disrupting QS, a strategy referred to as quorum quenching (QQ) can be achieved using exogenous AHL-degrading lactonases. However, the importance of enzyme specificity on quenching efficacy has been poorly investigated. Here, we used two lactonases both targeting the signal molecules N-(3-oxododecanoyl)-Lhomoserine lactone (3-oxo-C 12 HSL) and butyryl-homoserine lactone (C 4 HSL) albeit with different efficacies on C 4 HSL. Interestingly, both lactonases similarly decreased AHL concentrations and comparably impacted the expression of AHL-based QS genes. However, strong variations were observed in Pseudomonas Quinolone Signal (PQS) regulation depending on the lactonase used. Both lactonases were also found to decrease virulence factors production and biofilm formation in vitro, albeit with different efficiencies. Unexpectedly, only the lactonase with lower efficacy on C 4 HSL was able to inhibit P. aeruginosa pathogenicity in vivo in an amoeba infection model. Similarly, proteomic analysis revealed large variations in protein levels involved in antibiotic resistance, biofilm formation, virulence and diverse cellular mechanisms depending on the chosen lactonase. This global analysis provides evidences that QQ enzyme specificity has a significant impact on the modulation of QS-associated behavior in P. aeruginosa PA14.

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“…Succinyl CoA is also a precursor for protein succinylation, and such posttranslational modification is used to fine-tune the metabolism (Yang et al, 2015). In addition to proteins involved in TCA and gluconeogenesis, four proteins necessary for alginate biosynthesis, namely AlgU, MucB, AlgR, and AlgC, were found to be succinylated in P. aeruginosa (Gaviard et al, 2018).…”

Section: Alginate Biosynthesis Seems To Be Regulated By the Metabolicmentioning

confidence: 99%

Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis in Azotobacter vinelandii by Screening of a Transposon Insertion Mutant Library

Jakobsen

Sletta

Klinkenberg

et al. 2020

Front. Bioeng. Biotechnol.

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Azotobacter vinelandii produces the biopolymer alginate, which has a wide range of industrial and pharmaceutical applications. A random transposon insertion mutant library was constructed from A. vinelandii ATCC12518Tc in order to identify genes and pathways affecting alginate biosynthesis, and about 4,000 mutant strains were screened for altered alginate production. One mutant, containing a mucA disruption, displayed an elevated alginate production level, and several mutants with decreased or abolished alginate production were identified. The regulatory proteins AlgW and AmrZ seem to be required for alginate production in A. vinelandii, similarly to Pseudomonas aeruginosa. An algB mutation did however not affect alginate yield in A. vinelandii although its P. aeruginosa homolog is needed for full alginate production. Inactivation of the fructose phosphoenolpyruvate phosphotransferase system protein FruA resulted in a mutant that did not produce alginate when cultivated in media containing various carbon sources, indicating that this system could have a role in regulation of alginate biosynthesis. Furthermore, impaired or abolished alginate production was observed for strains with disruptions of genes involved in peptidoglycan biosynthesis/recycling and biosynthesis of purines, isoprenoids, TCA cycle intermediates, and various vitamins, suggesting that sufficient access to some of these compounds is important for alginate production. This hypothesis was verified by showing that addition of thiamine, succinate or a mixture of lysine, methionine and diaminopimelate increases alginate yield in the non-mutagenized strain. These results might be used in development of optimized alginate production media or in genetic engineering of A. vinelandii strains for alginate bioproduction.

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Lysine Succinylation and Acetylation in Pseudomonas aeruginosa

Cited by 66 publications

References 78 publications

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Lactonase Specificity Is Key to Quorum Quenching in Pseudomonas aeruginosa

Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis in Azotobacter vinelandii by Screening of a Transposon Insertion Mutant Library

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