Lysine acetylation participates in boar spermatozoa motility and acrosome status regulation under different glucose conditions

Chen, Guo; Ren, Li; Chang, Zhanglin; Zhao, Yuting; Zhang, Yanwen; Xia, Dong; Zhao, Ruqian

doi:10.1016/j.theriogenology.2020.10.027

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…Furthermore, a series of studies have shown that sperm motility and sperm progressive motility signify the function of mitochondria and energy status, which have been associated with fertility. 25,26 Studies have also shown that the VCL reflects the high-energy state of sperm and hyperactivated motility, which are essential for successful fertilization. 27,28 However, to a large extent, the short-term and cumulative effects of a HFD on these sperm parameters are controversial.…”

Section: Discussionmentioning

confidence: 99%

The time-dependent adverse effects of a high-fat diet on sperm parameters

Qi¹,

Guan²,

Zhang³

et al. 2023

Adv Clin Exp Med

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Background. Studies indicate a relationship between a high-fat diet (HFD) and sperm quality. However, the time-dependent adverse effects of a HFD on sperm parameters and the underlying mechanisms remain unclear.Objectives. The present study was designed to determine the effects of a HFD on sperm quality at various time points in order to assess whether a HFD causes cumulative damage to sperm. Materials and methods.Male C57BL/6 mice were fed a normal diet (the ND group) or a HFD (the HFD group) for 16, 30 or 42 weeks (n = 6 for each group). Body weight, lipid profile, sperm parameters, testicular morphology, and testicular oxidative stress levels were evaluated alongside the proliferation, DNA damage and rate of germ cell apoptosis.Results. Sperm quality was reduced in HFD-fed animals in a time-dependent manner, which was demonstrated by a decline in sperm density, motility and progressive motility. Further analysis showed a progressive deterioration of the testicular histoarchitecture of HFD-fed mice, which was accompanied by a decrease in DEAD-box helicase 4 (DDX4) expression and superoxide dismutase (SOD) levels, increased malondialdehyde (MDA) levels and gamma-H2A histone family member X (γ-H2AX) expression, and increased apoptosis of germ cells.Conclusions. These findings demonstrate that a HFD exerted adverse effects on sperm quality, and the deteriorating effect was progressive with long-term feeding. The inhibited proliferation and apoptosis of germ cells, and the increased oxidative stress levels and DNA damage may be the underlying mechanisms.

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Section: Discussionmentioning

confidence: 99%

The time-dependent adverse effects of a high-fat diet on sperm parameters

Qi¹,

Guan²,

Zhang³

et al. 2023

Adv Clin Exp Med

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“…Independently of sirtuins localization, it is clear that protein acetylation pathway plays a role on both sperm processes: acrosome reaction (Chen et al 2021 ; Bowker et al 2022 ) and capacitation (Sun et al 2014 ; Yu et al 2015 ; Ritagliati et al 2018 ), highlighting the possible role of the sirtuin family in the fertilization process. In addition, SIRT1-deficient mice induce male infertility by inducing spermiogenesis disruption, sperm morphological abnormalities (McBurney et al 2003 ; Coussens et al 2008 ; Bell et al 2014 ) and the inability to display hyperactivated motility (Iniesta-Cuerda et al 2022a ).…”

Section: Discussionmentioning

confidence: 99%

The compound YK 3-237 promotes pig sperm capacitation-related events

Martín-Hidalgo,

Solar-Málaga,

González-Fernández

et al. 2023

Vet Res Commun

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Before fertilization of the oocyte, the spermatozoa must undergo through a series of biochemical changes in the female reproductive tract named sperm capacitation. Spermatozoa regulates its functions by post-translational modifications, being historically the most studied protein phosphorylation. In addition to phosphorylation, recently, protein acetylation has been described as an important molecular mechanism with regulatory roles in several reproductive processes. However, its role on the mammal’s sperm capacitation process remains unraveled. Sirtuins are a deacetylase protein family with 7 members that regulate protein acetylation. Here, we investigated the possible role of SIRT1 on pig sperm capacitation-related events by using YK 3-237, a commercial SIRT1 activator drug. SIRT1 is localized in the midpiece of pig spermatozoa. Protein tyrosine phosphorylation (focused at p32) is an event associated to pig sperm capacitation that increases when spermatozoa are in vitro capacitated in presence of YK 3-237. Eventually, YK 3-237 induces acrosome reaction in capacitated spermatozoa: YK 3-237 treatment tripled (3.40 ± 0.40 fold increase) the percentage of acrosome-reacted spermatozoa compared to the control. In addition, YK 3-237 induces sperm intracellular pH alkalinization and raises the intracellular calcium levels through a CatSper independent mechanism. YK 3-237 was not able to bypass sAC inhibition by LRE1. In summary, YK 3-237 promotes pig sperm capacitation by a mechanism upstream of sAC activation and independent of CatSper calcium channel.

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“…Dietary choline was required for sperm motility in Drosophila [ 33 ]. Lysine acetylation was involved in regulating boar sperm motility and acrosome integrity [ 34 ], and inducing a mouse sperm acrosome reaction [ 35 ]. Alanine addition improved the post-thaw motility of striped bass sperm [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa

Sui

Wang

Liu

et al. 2022

Genes

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Heat stress (HS) commonly causes boar infertility and economic loss in the swine industry. The heat tolerance of boar semen presents obvious differences among individuals. However, whether heat stress affects motion characteristics and the metabolome profile in boar sperm remains unclear. In this study, the kinetic features of sperm from HS and non-HS (NHS) groups were detected by computer-assisted sperm analysis, and metabolomic profiling was performed by liquid chromatography–mass spectrometry. The results showed that heat stress significantly reduced sperm motility, average path distance (APD), straight-line velocity (VSL), straightness (STR), and linearity (LIN) (p < 0.05). A total of 528 and 194 metabolites in sperm were identified in the positive and negative ion modes, respectively. Lipids and lipid-like molecules, and organic acids and derivatives were major metabolic classes in the two modes. Furthermore, we separately identified 163 and 171 differential metabolites in the two modes between HS and NHS groups. Clustering analysis further revealed significant metabolic changes in sperm after heat stress. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differential metabolites in the two modes were enriched in glycerophospholipid, choline, and alanine, aspartate, and glutamate and lysine metabolism. Taken together, these results demonstrate that heat stress can alter the motion characteristics and metabolomic profiles of boar sperm.

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Lysine acetylation participates in boar spermatozoa motility and acrosome status regulation under different glucose conditions

Cited by 11 publications

References 23 publications

The time-dependent adverse effects of a high-fat diet on sperm parameters

The time-dependent adverse effects of a high-fat diet on sperm parameters

The compound YK 3-237 promotes pig sperm capacitation-related events

Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa

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