Lyotropic Behavior of a Mono-tailed Glycolipid Assembly during Solidification and Melting of Electrolyte/Ice Eutectic Systems

Abstract

The properties of low molecular weight glycolipids at subzero temperatures have been paid much attention due to their great potential for applications in cryopreservation. In this study, the ability of glycolipids to maintain their lyotropic properties was investigated by analyzing the aggregation behavior of octyl-β-Dglucoside (β-OGlu) during solidification and melting of electrolyte/ice eutectic mixtures using a simultaneous X-ray diffraction-differential scanning calorimetry measurement and cryomicroscopic observation. During the freezing process, eutectic formation increased the concentration of the β-OGlu micelle solution in the unfrozen phase, leading to ordering of the molecular assembly. The molecular assembly exhibited a reversible phase transformation between liquid crystalline phases (e.g., lamellar (Lα) and hexagonal (H) phases) and an isotropic micelle solution (I), depending on the degree of eutectic formation, which was regulated by temperature. Because the coexistence of electrolyte/ice eutectic often induces the dehydration of lipids, the persistence of lyotropic properties under such arid conditions demonstrates an attractive performance of glycolipids for use in low temperature applications, such as the cryopreservation of poorly water-soluble drugs or proteins.

Tuning the Complex Spherical Phase of Sugar-Based Block Co-Oligomer via Single-Monomer-Mediated Composition Variation

“…The distinctive chemical structure of glycolipids, characterized by a hydrophilic saccharide moiety and short alkyl chains, could serve as a valuable clue for designing a high-χ/low- N BCO capable of self-assembling into well-ordered nanostructures. While much of the research of glycolipids has focused on their lyotropic phase behavior in aqueous environments, − the thermotropic phase behavior has also received attention. − Glycolipids form periodic nanostructures in a liquid crystalline state between the melting point of the alkyl chain and the clearing temperature. The hydrogen bonding interactions between the glucose units are crucial in determining the type and thermal stability of the structures formed.…”

Oligosaccharide-based Surfactant/Citric Acid Buffer System Stabilizes Lactate Dehydrogenase during Freeze-drying and Storage without the Addition of Natural Sugar

“…Concerning their application for protein preservation during freeze-drying, sugar-based surfactants have been reported to effectively prevent the inactivation aqueous natural sugar solution 18,19 . In addition, a typical sugar-based surfactant is less likely to crystallize itself under arid conditions 20 . These properties are naturally received as important functions for protein stabilization during both freeze-drying and storage.…”

Aqueous Sugar-Based Amphiphile Systems: Recent Advances in Phase Behavior and Nanoarchitectonics

“…(e) Temperature-dependent peak intensities of Cr and LC for C8Glc 41) . electrolyte such as NaCl and KCl, detailed analysis became difficult, but the persistence of the lyotropic behavior of C8Glc without precipitation in a freezing state was confirmed by simultaneous X-ray diffraction (XRD) -DSC measurements and low-temperature XRD analysis 32,36,37) .…”