LyophilizedCarnobacterium divergens AS7 bacteriocin preparation improves performance of broiler chickens challenged withClostridium perfringens

Józefiak, Damian; Sip, A.; Rutkowski, A.; Rawski, Mateusz; Kaczmarek, S.; Wołuń-Cholewa, Maria; Engberg, Ricarda M.; Højberg, Ole

doi:10.3382/ps.2012-02151

Cited by 28 publications

(16 citation statements)

References 30 publications

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“…This could also be proven by the lower ADG and higher FCR of infected birds during the period of infection in the current experiment. This was in agreement with the results of Yitbarek et al (2012) and Józefiak et al (2012), which suggested that the C. perfringens challenge decreased BW gain and impaired FCR throughout the experimental period despite different challenge models employed with minor distinctions.…”

Section: Discussionsupporting

confidence: 93%

“…Subclinical NE, imposing a significant economic burden on the poultry industry, was first described by Kaldhusdal and Hofshagen (1992). In this form of NE, chronic intestinal mucosal damage leads to production losses due to poor digestion and absorption, reduced weight gain, and increased feed conversion ratio (FCR; Elwinger et al, 1992;Kaldhusdal et al, 2001;Jia et al, 2009;Józefiak et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Xylanase supplementation of a wheat-based diet improved nutrient digestion and mRNA expression of intestinal nutrient transporters in broiler chickens infected with Clostridium perfringens

et al. 2014

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Necrotic enteritis caused by Clostridium perfringens has become prevalent in the European Union due to the withdrawal of antibiotics in poultry feed. In an experiment with a 2 × 2 factorial arrangement, 336 one-day-old male broiler chicks (Ross 308) were assigned to 4 groups with or without C. perfringens challenge and fed wheat-based diets supplemented with or without xylanase at 5,500 U/kg of diet. The study aimed to investigate effects of xylanase addition on growth performance as well as nutrient digestion and absorption of C. perfringens-infected broilers. Before challenge (d 0-14), xylanase-supplemented birds had greater ADG and lower feed conversion ratio (FCR; P < 0.05). During infection (d 14-21), challenge tended to decrease ADG (P = 0.063) and significantly increased FCR (P < 0.05), whereas xylanase addition greatly reduced FCR (P < 0.05). Clostridium perfringens infection decreased AME values and apparent ileal digestibility of DM of diets (P < 0.05). Xylanase supplementation increased AME values regardless of infection and apparent ileal digestibility of CP in challenged birds (P < 0.05). Activities of duodenal α-amylase and chymotrypsin and pancreatic trypsin were decreased by C. perfringens infection (P < 0.05). Xylanase supplementation elevated pancreatic chymotrypsin activity and reduced duodenal α-amylase and trypsin activities (P < 0.05). It also decreased jejunal α-amylase activity and increased pancreatic α-amylase as well as jejunal sucrase activities in uninfected birds (P < 0.05). The duodenal mRNA expression of sodium glucose cotransporter 1 (SGLT1), H(+)-dependent peptide transporter 1 (PepT1), and liver fatty acid-binding protein (L-FABP) were downregulated (P < 0.05), but ileal SGLT1 gene expression was increased by infection (P < 0.05). Xylanase addition upregulated expression of jejunal SGLT1, PepT1, and L-FABP genes as well as ileal PepT1 and L-FABP genes in challenged broilers (P < 0.05). In conclusion, xylanase supplementation of wheat-based diets improved FCR and AME in birds irrespective of C. perfringens infection and elevated apparent ileal digestibility of CP and mRNA expression of nutrient transporters in challenged birds.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Xylanase supplementation of a wheat-based diet improved nutrient digestion and mRNA expression of intestinal nutrient transporters in broiler chickens infected with Clostridium perfringens

et al. 2014

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“…However in the ceca, inhabited mostly by strict anaerobes, lactobacilli accounted for only 8% of the library sequences [35,58]. In human and animal nutrition, LAB including Lactobacillus genus are usually considered beneficial for the host, mainly because they produce lactic and acetic acids, which leads to pH reduction [59,60] and certain bacteriocins, which help fighting pathogenic bacteria [61,62]. On the other hand, there is also an evidence that some small intestinal LAB, e.g.…”

Section: Discussionmentioning

confidence: 99%

Synbiotics for Broiler Chickens—In Vitro Design and Evaluation of the Influence on Host and Selected Microbiota Populations following In Ovo Delivery

et al. 2017

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Synbiotics are synergistic combinations of prebiotics and probiotics. In chickens, synbiotics can be delivered in ovo to expedite colonization of the gut by beneficial bacteria. We therefore aimed to design synbiotics in vitro and validate them in broiler chickens upon in ovo delivery. The probiotic components of the synbiotics were Lactobacillus salivarius and Lactobacillus plantarum. Their growth was assessed in MRS medium supplemented with different prebiotics. Based on in vitro results (hatchability and growth curve), two synbiotics were designed: S1 –Lactobacillus salivarius with galactooligosaccarides (GOS) and S2 –Lactobacillus plantarum with raffinose family oligosaccharides (RFO). These synbiotics were delivered to Cobb broiler chicken embryos on day 12 of incubation at optimized doses (105 cfu egg-1 of probiotic, 2 mg egg-1 of prebiotic). Post hatching, 2,400 roosters were reared (600 individuals group-1 divided into eight replicate pens). Microbial communities were analyzed in ileal and cecal digesta on day 21 using FISH. Gene expression analysis (IL1β, IL4, IL6, IL8, IL12, IL18, IFNβ, and IFNγ) was performed on days 7, 14, 21, and 42 for the spleen and cecal tonsils with RT-qPCR. Body weight and feed intake of the roosters did not differ by the treatments. Microbial populations of Lactobacillus spp. and Enterococcus spp. in the ileum were higher in S1 and S2 than in the control. In the cecum, the control had the highest bacterial counts. S1 caused significant up-regulation of IL6, IL18, IL1β, IFNγ, and IFNβ in the spleen on day 21 and IL1β on day 7 (P < 0.05). In cecal tonsils, S1 caused significant down-regulation of IL12, IL8, and IL1β on day 42 and IFNβ on day 14 (P < 0.05). S2 did not elicit such patterns in any tissues investigated. Thus, we demonstrate that divergent effects of synbiotics in broiler chickens were reflected in in vitro tests.

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“…Moreover, in many experiments carried out on broiler chickens, where probiotic strains were applied, the potential production of bacteriocins by these microorganisms and their impact on the GIT homeostasis was not investigated as a key factor. Meanwhile, earlier studies conducted on different bacteriocins suggests that divercin AS7 may compensate the negative effects associated with the presence of C. perfringens, while nisin supplementation enhanced growth performance of the birds through modulation of GIT microecology (Józefiak et al, 2012.…”

Section: Discussionmentioning

confidence: 99%

The nisin improves broiler chicken growth performance and interacts with salinomycin in terms of gastrointestinal tract microbiota composition

Kierończyk¹,

Pruszyńska‐Oszmałek²,

Świątkiewicz³

et al. 2016

J. Anim. Feed Sci.

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LyophilizedCarnobacterium divergens AS7 bacteriocin preparation improves performance of broiler chickens challenged withClostridium perfringens

Cited by 28 publications

References 30 publications

Xylanase supplementation of a wheat-based diet improved nutrient digestion and mRNA expression of intestinal nutrient transporters in broiler chickens infected with Clostridium perfringens

Xylanase supplementation of a wheat-based diet improved nutrient digestion and mRNA expression of intestinal nutrient transporters in broiler chickens infected with Clostridium perfringens

Synbiotics for Broiler Chickens—In Vitro Design and Evaluation of the Influence on Host and Selected Microbiota Populations following In Ovo Delivery

The nisin improves broiler chicken growth performance and interacts with salinomycin in terms of gastrointestinal tract microbiota composition

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