Lyophilized tomato plant material: Validation of a reliable extraction method for the analysis of vitamin C

Sérino, Sylvie; Costagliola, Guy; Gomez, Laurent

doi:10.1016/j.jfca.2019.05.001

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…Vit C extraction from the freeze-dried samples was performed using 6% trichloroacetic acid as described by Sérino et al 48 , who adapted Vit C analysis protocols for microplate spectroscopy. Following the extraction, 20 µL of each sample was added to the microplate and reacted using dithiothreitol (DTT) to reduce oxidized ascorbate.…”

Section: Methodsmentioning

confidence: 99%

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Increasing vitamin C through agronomic biofortification of arugula microgreens

Kathi

Laza

Singh

et al. 2022

Sci Rep

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Vitamin C (Vit C) is an essential micronutrient and antioxidant for human health. Unfortunately, Vit C cannot be produced in humans and is ingested through diet while severe deficiencies can lead to scurvy. However, consumption is often inconsistent, and foods vary in Vit C concentrations. Biofortification, the practice of increasing micronutrient or mineral concentrations, can improve the nutritional quality of crops and allow for more consistent dietary levels of these nutrients. Of the three leading biofortification practices (i.e., conventional, transgenic, and agronomical), the least explored approach to increase Vit C in microgreens is agronomically, especially through the supplemental application of ascorbic acid. In this study, biofortification of Vit C in microgreens through supplemental ascorbic acid was attempted and proven achievable. Arugula (Eruca sativa 'Astro') microgreens were irrigated with four concentrations of ascorbic acid and a control. Total Vit C (T-AsA) and ascorbic acid increased in microgreens as supplementary concentrations increased. In conclusion, biofortification of Vit C in microgreens through supplemental ascorbic acid is achievable, and consumption of these bio-fortified microgreens could help fulfill the daily Vit C requirements for humans, thereby reducing the need for supplemental vitamins.

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Section: Methodsmentioning

confidence: 99%

“…Samples were prepared simultaneously for both T-AsA and AsA and absorbance was read at 550 nm using the microplate spectrophotometer (SpectroMax iD3, San Jose, California). Total Vit C and AsA 100 g −1 FW were calculated according to Sérino et al 48 .…”

Section: Methodsmentioning

confidence: 99%

Increasing vitamin C through agronomic biofortification of arugula microgreens

Kathi

Laza

Singh

et al. 2022

Sci Rep

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“…Total glutathione was quantified as the GSSG equivalent by enzymatic assay (Massot et al 2013 ). The reduced and oxidized forms of ascorbate were quantified by enzymatic assay (Sérino et al 2019 ). Starch was measured by enzymatic assay in glucose equivalents (Biais et al 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Nitrogen-mediated metabolic patterns of susceptibility to Botrytis cinerea infection in tomato (Solanum lycopersicum) stems

Lacrampe

Colombié

Dumont

et al. 2023

Planta

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Main conclusion Severe N stress allows an accumulation of C-based compounds but impedes that of N-based compounds required to lower the susceptibility of tomato stem toBotrytis cinerea. Abstract Botrytis cinerea, a necrotrophic filamentous fungus, forms potentially lethal lesions on the stems of infected plants. Contrasted levels of susceptibility to B. cinerea were obtained in a tomato cultivar grown on a range of nitrate concentration: low N supply resulted in high susceptibility while high N supply conferred a strong resistance. Metabolic deviations and physiological traits resulting from both infection and nitrogen limitation were investigated in the symptomless stem tissue surrounding the necrotic lesion. Prior to infection, nitrogen-deficient plants showed reduced levels of nitrogen-based compounds such as amino acids, proteins, and glutathione and elevated levels of carbon-based and defence compounds such as α-tomatine and chlorogenic acid. After B. cinerea inoculation, all plants displayed a few common responses, mainly alanine accumulation and galactinol depletion. The metabolome of resistant plants grown under high N supply showed no significant change after inoculation. On the contrary, the metabolome of susceptible plants grown under low N supply showed massive metabolic adjustments, including changes in central metabolism around glutamate and respiratory pathways, suggesting active resource mobilization and production of energy and reducing power. Redox and defence metabolisms were also stimulated by the infection in plants grown under low N supply; glutathione and chlorogenic acid accumulated, as well as metabolites with more controversial defensive roles, such as polyamines, GABA, branched-chain amino acids and phytosterols. Taken together, the results showed that nitrogen deficiency, although leading to an increase in secondary metabolites even before the pathogen attack, must have compromised the constitutive levels of defence proteins and delayed or attenuated the induced responses. The involvement of galactinol, alanine, cycloartenol and citramalate in the tomato stem response to B. cinerea is reported here for the first time.

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“…The Vitamin C extraction of the lyophilized leaves was performed using 6% trichloroacetic acid adapted from Sérino et al [57]. The analysis was performed using microplate spectroscopy [57].…”

Section: Vitamin C Extraction and Analysismentioning

confidence: 99%

Growth of Romaine Lettuce in Eggshell Powder Mixed Alginate Hydrogel in an Aeroponic System for Water Conservation and Vitamin C Biofortification

Afnan,

Kashem,

Joshi

et al. 2024

Gels

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Vitamin C is crucial for physical well-being, and its deficiency can lead to severe health consequences. Biofortification has been used to address this deficiency by enhancing vitamin C in plants. Additionally, soilless agriculture has been used to conserve and optimize water use in comparison to conventional agriculture. While hydrogels have been shown to improve water conservation and are used for biofortification in crops, their application has only been explored in soil-based and hydroponic farming. The aeroponics system is a plant-growing method that has shown potential for increasing yields and biomass while conserving water and nutrients. In this paper, we have developed an aeroponic-compatible medium to grow romaine lettuce (Lactuca sativa L.) with eggshell powder (ESP) mixed with calcium-alginate hydrogel as a substrate and nutrient source aiming to conserve water and incorporate vitamin C through biofortification. Herein, lower water spray time and higher intervals, with varied gel types and ESP concentrations, resulted in healthy lettuce growth. Plants treated with 0.5% ascorbic acid-absorbed ESP-mixed alginate hydrogel for biofortification showed higher levels of vitamin C compared to the traditional method. This study suggests using an alginate hydrogel–ESP-based substrate in aeroponics to reduce water usage and enhance plant biofortification of vitamin C.

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Lyophilized tomato plant material: Validation of a reliable extraction method for the analysis of vitamin C

Cited by 10 publications

References 30 publications

Increasing vitamin C through agronomic biofortification of arugula microgreens

Increasing vitamin C through agronomic biofortification of arugula microgreens

Nitrogen-mediated metabolic patterns of susceptibility to Botrytis cinerea infection in tomato (Solanum lycopersicum) stems

Growth of Romaine Lettuce in Eggshell Powder Mixed Alginate Hydrogel in an Aeroponic System for Water Conservation and Vitamin C Biofortification

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