Lyophilized Platelet-Rich Fibrin (PRF) Promotes Craniofacial Bone Regeneration through Runx2

Li, Qi; Reed, David A.; Liu, Min; Gopinathan, Gokul; Li, Steve; Dangaria, Smit; Li, Leo; Geng, Yajun; Galang, Maria-Therese; Gajendrareddy, Praveen; Zhou, Yanmin; Luan, Xianghong; Diekwisch, Thomas G.H.

doi:10.3390/ijms15058509

Cited by 69 publications

(81 citation statements)

References 37 publications

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“…The expression of BSP-I increased gradually from Day 2 to Day 7. These results were in line with those of Li et al and Tu et al which posited that BSP-I expression constituted a late stage osteogenic differentiation marker [15,16]. The addition of PRF to GSC culture medium during osteogenic differentiation can stimulate BSP-I expression, a position adopted in the study by Duan et al that showed significantly increased BSP-I or Osteopontin expression in combined human GSC and PRF on Day 21 [10].…”

Section: Discussionsupporting

confidence: 88%

<i>In vitro</i> bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

Nugraha¹,

Narmada²,

Ernawati³

et al. 2019

Trop. J. Pharm Res

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Purpose:To analyze the expression of bone sialoprotein -I (BSP -I) after the addition of platelet rich fibrin (PRF) in gingival somatic cell (GSC) culture medium during osteogenic differentiation in vitro. Methods: GSCs were extracted from healthy, 1-month-old, male Wistar rats (Rattus Novergicus), weighing 250 -300 g, and which had been randomly selected (n=4). These cells were cultured for 14 days and passaged every 4 days. Five subcultures of GSCs were cultured in three plates (M24) (N = 54; n = 6) for 7, 14 and 21 days in three preconditioned culture media (group I: plain culture media; group II: preconditioned osteogenic culture media, and group III: preconditioned osteogenic culture media with platelet rich fibrin). The expression of BSP-I was immunocytochemically (ICC) examined with monoclonal antibodies. Homogeneity and normality tests (p > 0.05) were then performed followed by an analysis of variance (ANOVA, p < 0.05). Results: The highest expression of BSP-I was found in group III (Day 21,13.00 ± 2.000), while the lowest expression of BSP-I was found in group I (Day 7, 7.33 ± 1.155). There were significant differences between the groups (p = 0.000, p < 0.05). Conclusion: PRF stimulates and significantly enhances the expression of BSP-I in GSC culture during osteogenic differentiation. Thus, PRF can be used to accelerate regeneration of alveolar bone defects. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

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Section: Discussionsupporting

confidence: 88%

<i>In vitro</i> bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

Nugraha¹,

Narmada²,

Ernawati³

et al. 2019

Trop. J. Pharm Res

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“…The ELISA test that was performed showed the potential release of growth factor (IGF-1) by L-PRF. The release of the growth factors was in a continuous manner up to 14 days, as reported in many published studies [27][28][29][30] . The composition of the fibrin network allows a slow but high-level of fibrillar aggregation in L-PRF, entrapping proteins and growth factors to the binding domains of fibrin molecules at the same time.…”

Section: Discussionmentioning

confidence: 59%

The Effects of Leukocyte-Platelet Rich Fibrin (L-PRF) on Suppression of the Expressions of the Pro-Inflammatory Cytokines, and Proliferation of Schwann Cell, and Neurotrophic Factors

Wang

Mudalal

Sun

et al. 2020

Sci Rep

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This study evaluates the use of L-PRF as an autologous scaffold in nerve regeneration, and Schwann cells (SCs) proliferation and secretion of neurotrophic factors and its anti-inflammatory effect on SC Porphyromonas Gingivalis-Lipopolysaccharide (PG-LPS)-induced inflammatory responses in vitro. SEM was done to investigate various features of L-PRF. L-PRF-extracts was used to investigate the release of growth factors and treatment of SCs line. ELISA was applied to examine the release of IGF-1. The proliferative effect of L-PRF on SCs was assessed with CCK-8 assay. The effect of L-PRF on the mRNA and protein expression of SC neurotrophic factors were analyzed by RT-qPCR and ELISA. CCK-8 assay and RT-qPCR were used to determine the required concentration and the action time of PG-LPS before the anti-inflammatory effect of L-PRF was determined by measuring the changes in IL-1β, IL-6, and TNF-a with RT-qPCR and ELISA. There are different features in L-PRF. Fourteen days was sufficient to release adequate GF. The mRNA expressions of the pro-inflammatory cytokines were notably raised by PG-LPS in 3-hours treatment. L-PRF can increase SC proliferation, neurotrophic factors secretion, and suppress SC PG-LPS-induced inflammatory responses in vitro. L-PRF has the potential as an autologous biological additive for peripheral nerve regeneration in the event of nerve inflammation and injuries. Recently, dental implantation has become the preferred option to replace missing teeth. However, patients who undergo implant treatment frequently suffer from severe postsurgical complications, especially nerve injury which can cast negative impacts on patients' quality of life and work 1. Clinically, nerve injury is characterized by the sensory and functional disorder in the dominant area, such as alteration in the sensation of the mucosa, lower lip, and chin and the feeling can range from slight numbness to complete anesthesia 2,3. At present, the solution for nerve injury includes intensive treatment protocols such as medications and physical therapy. Generally speaking, the first-line treatment for nerve injury combines both pharmacological intervention and physiotherapy 4. Medication used for nerve injury includes oral steroids to resolve neuritis and edema, vitamin B12 for the regeneration of nerve terminals, and adenosine triphosphate (ATP) to encourage blood flow via vasodilation 5. Physiotherapy such as the use of laser and hot pack treatment is also useful for vasodilation to increase the blood flow 5. However, Kim et al. 4 reported that these therapies are not full proof in providing positive outcomes in the treatment of nerve injury. After medication and physiotherapy for inferior alveolar nerve injury, as high as 7 in 10 patients reported no improvement in sensation. In other words, they still experience the same level of dysesthesia.

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“…CGF was mechanically separated using scissors and then compressed into a thin membrane. Surface parameters such as topography and hardness markedly affect cell behavior and the release of growth factors (10,12,23). SEM analysis (Fig.…”

Section: Resultsmentioning

confidence: 99%

Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway

Qin

Wang

Zheng

et al. 2016

International Journal of Molecular Medicine

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Nerve injury is a serious complication associated with dental implant surgery. Following nerve injury, the migration of Schwann cells (SCs) supports nerve regeneration. Concentrated growth factor (CGF) belongs to a new generation of biomaterials that contain fibrin matrix, as well as a number of growth factors that affect the migration of various types of cells, including endothelial cells and cancer cells. To the very best of our knowledge, there are no available studies to date on the promoting effect of CGF on the migration of SCs. Thus, the aim of the present study was to characterize the structure of CGF and growth factor release, examine the effects of CGF on the migration of SCs, and to examine the role of integrin β1 and the focal adhesion kinase (FAK) pathway in the CGF-induced migration of SCs. For this purpose, CGF was prepared by centrifuging rat venous blood, which produced a fiber-like matrix capable of releasing transforming growth factor-β1 (TGF-β1) over a sustained period of time (at least 13 days). The soluble component of CGF was used to produce conditioned media to treat the SC cells in culture. The results demonstrated that CGF promoted the migration of SCs and increased the expression of integrin β1. These effects appeared to involve FAK phosphorylation, which occurred downstream of integrin β1 activation. The short-interfering RNA (siRNA)-mediated downregulation of integrin β1 expression did not block the ability of CGF to promote the migration of SCs. These data suggest that CGF promotes the migration of SCs partly through the integrin β1-mediated activation of the FAK pathway.

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Lyophilized Platelet-Rich Fibrin (PRF) Promotes Craniofacial Bone Regeneration through Runx2

Cited by 69 publications

References 37 publications

<i>In vitro</i> bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

<i>In vitro</i> bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

The Effects of Leukocyte-Platelet Rich Fibrin (L-PRF) on Suppression of the Expressions of the Pro-Inflammatory Cytokines, and Proliferation of Schwann Cell, and Neurotrophic Factors

Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway

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