Lyophilized chitosan sponges

Berretta, Joel M; Bumgardner, Joel D.; Jennings, Jessica Amber

doi:10.1016/b978-0-08-100230-8.00010-8

Cited by 10 publications

(8 citation statements)

References 88 publications

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“…e drug release takes place over time as long as the chitosan swells [5][6][7]. e easy loading of drugs is attributable in part to chitosan's cationic nature, which promotes swelling and electrostatic interactions between the polymer and aqueous proteins and drugs that have a net negative charge.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Lyophilized Chitosan-Based Hydrogels Cross-Linked with Benzaldehyde for Controlled Drug Release

Damiri

Bachra

Bounacir

et al. 2020

Journal of Chemistry

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In this study, chitosan-based hydrogels were produced by incorporating three drugs with a different solubility into a polymer matrix. The lyophilized chitosan salt was prepared using an innovative and less-expensive synthetic process by the freeze-drying technique. Firstly, the three drugs (caffeine, ascorbic acid, and 5-fluorouracil (5-FU)) were selected as model drugs to test the in vitro release behavior of the hydrogel. The drugs were solubilized in chitosan salt, lyophilized, and cross-linked with benzaldehyde involving the formation of a Schiff base with (–C=N-) linkage to produce a physical hydrogel. Subsequently, the physicochemical properties of N-benzyl chitosan and lyophilized chitosan salt were evaluated by Fourier-transform infrared (FTIR) spectra, scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The intrinsic viscosity of the conventional chitosan was determined by the Mark–Houwink–Sakurada equation. Moreover, the kinetics of hydrogel swelling and drug release were studied by the UV-visible method at physiological conditions (pH = 7.4 at 37°C). The results show that lyophilized N-benzyl chitosan had a maximum swelling ratio of 720 ± 2% by immersion in phosphate-buffered saline solutions (PBS) (pH = 7.4 at 37°C). In vitro drug releases were evaluated in PBS, and the obtained results show that the maximum drug release after 24 h was 42% for caffeine, 99% for 5-FU, and 94% for ascorbic acid. Then, to optimize the cumulative release of caffeine, Tween 20 was added and 98% as a release percentage was obtained. The drug-loading results were investigated with the Korsmeyer–Peppas kinetic model and applied to determine the drug release mechanism.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Lyophilized Chitosan-Based Hydrogels Cross-Linked with Benzaldehyde for Controlled Drug Release

Damiri

Bachra

Bounacir

et al. 2020

Journal of Chemistry

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“…Increasing CS concentrations in the solution result in thicker walls, reducing the porosity. 27 Through this preliminary study, 8% CS was chosen to continue on because of its optimal pore characteristic. The optimal pore size can vary from 100 to 300 μm for tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Development and Characterization of a Biocomposite Material from Chitosan and New Zealand-Sourced Bovine-Derived Hydroxyapatite for Bone Regeneration

et al. 2020

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A biocomposite scaffold was developed using chitosan (CS) and bovine-derived hydroxyapatite (BHA). The prepared CS–BHA biocomposite scaffold was characterized for its physiochemical and biological properties and compared against control BHA scaffolds to evaluate the effects of CS. Energy-dispersive X-ray analysis confirmed the elemental composition of the CS–BHA scaffold, which presented peaks for C and O from CS and Ca and P along with trace elements in the bovine bone such as Na, Mg, and Cl. Fourier transform infrared spectroscopy confirmed the presence of phosphate, hydroxyl, carbonate, and amide functional groups attributed to the CS and BHA present in the biocomposite scaffolds. The CS–BHA scaffolds demonstrated an interconnected porous structure with pore sizes ranging from 60 to 600 μm and a total porosity of ∼64–75%, as revealed by scanning electron microscopy and micro-CT analyses, respectively. Furthermore, thermogravimetric analysis revealed that the CS–BHA scaffold lost 70% of its weight when heated up to 1000 °C, which is characteristic of CS phase decomposition in the biocomposite. In vitro studies demonstrated that the CS–BHA scaffolds were biocompatible toward Saos-2 osteoblast-like cells, showing high cell viability and a significant increase in cell proliferation across the measured timepoints compared to the controls.

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“…Ocular inserts were prepared by lyophilization method [19]. Briefly, chitosan was dissolved (40 mg/mL) in acetic acid solution (2%, v/v), and posaconazole was dissolved in ethanol:acetonitrile (5:1) mixture.…”

Section: Preparation Of Ocular Insertsmentioning

confidence: 99%