Lyophilization of Protein-Loaded Polyelectrolyte Microcapsules

Abstract

Lyophilization is a promising strategy towards improved stability of protein-loaded microcapsules.

Micro and Nanoparticle‐Based Delivery Systems for Vaccine Immunotherapy: An Immunological and Materials Perspective

“…Comparable results were observed when CpG was incorporated into the DS particles 132. De Temmerman et al showed that these results are not altered significantly by lyophilization of antigen encapsulated DS particles, making them a promising option for an effective, storable vaccine 133. Tetanus toxoid encapsulated cross‐linked dextran particles were also able to generate well over protective levels of neutralizing antibodies 134…”

Protein microparticles with controlled stability prepared via layer-by-layer adsorption of biopolyelectrolytes

“…Under physiological conditions, the gradual release of the model ovalbumin lasted for 8 days, and the amount of the released protein decreased with an increase in the number of bilayers. This circumstance is important for the development of long acting vaccines [69][70][71]. During encapsulation of bovine serum albumin, the concentration of protein inside microcapsules was 100 mg/ml after formation of three to five polylysine and alginate bilayers.…”

“…To prepare stable drugs on the basis of protein poly electrolyte microparticles, optimum conditions were found for drying of preparations, their storage, and their subsequent resuspension without loss of the shape and integrity of particles and the biological activity of the encapsulated BAC [71].…”

“…The ability of such particles to release protein in a con trolled manner in the specified pH range may serve as a basis for creation of peroral forms for insulin delivery [54,55,63]. Another direction important for use in practice is the preparation of microcapsules of a fixed diameter that are formed on carbonate cores [69][70][71]. The applicability of similar microcapsules for the manufacture of long acting vaccines makes these studies especially topical.…”

Protein‐Containing Multilayer Capsules by Templating on Mesoporous CaCO3 Particles: POST‐ and PRE‐Loading Approaches

“…Even the morphology of calcium carbonate particles affects the loading, while the loading and the release are relevant for pharmacological applications . Despite a large number of articles devoted to protein loading into polymer‐based multilayer capsules, there is still no full understanding of the mechanism of the protein loading. For instance, the mechanism of protein embedding by co‐synthesis is still not reported in literature.…”