Lymphoscintigraphy of melanoma: Lymphatic channel activity guides localization of sentinel lymph nodes, and gamma camera imaging/counting confirms presence of radiotracer in excised nodes

Wj, Shih; Da, Sloan; Mt, Hackett; Uy, Ryo; Weizbinski, B; Jj, Coupal; Magoun, Sylvia

doi:10.1007/bf03012124

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Lymphoscintigraphy is more commonly used in the clinic, which utilizes radioactive tracers such as 99m ‐technetium ( 99m ‐Tc) injected in the tissue . The tracer drained through the lymphatic system can be imaged with a scintillation camera that integrates the signal over time to produce a 2D image of the lymphatic network . However, its poor spatial resolution does not allow clear identification of lymphatic vessels.…”

Section: Introductionmentioning

confidence: 99%

Lymphatic response to depilation‐induced inflammation in mouse ear assessed with label‐free optical lymphangiography

2015

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Background and Objectives Optical microangiography (OMAG) is a noninvasive technique capable of imaging 3D microvasculature. OMAG-based optical lymphangiography has been developed for 3D visualization of lymphatic vessels without the need for exogenous contrast agents. In this study, we utilize the optical lymphangiography to investigate dynamic changes in lymphatic response within skin tissue to depilation-induced inflammation by using mouse ear as a simple tissue model. Materials and Methods A spectral-domain optical coherence tomography (OCT) system is used in this study to acquire volumetric images of mouse ear. The system operates under the ultrahigh-sensitive OMAG scanning protocol with 5 repetitions for each B frame. An improved adaptive-threshold-based method is proposed to segment lymphatic vessels from OCT microstructure images. Depilation is achieved by placing hair removal lotion on mouse ear pinna for 5 minutes. 3 acquisitions are made before depilation, 3-minute and 30-minute post-depilation, respectively. Results Right after the application of depilation lotion on the skin, we observe that the blind-ended sacs of initial lymphatics are mainly visible in a specific area of the normal tissue. At 5 minutes, more collecting lymphatic vessels start to form, evidenced by their valve structure that only exists in collecting lymphatic vessels. The lymphangiogenesis is almost completed within 8 minutes in the inflammatory tissue. Conclusions Our experimental results demonstrate that the OMAG-based optical lymphangiography has great potential to improve the understanding of lymphatic system in response to various physiological conditions, thus would benefit the development of effective therapeutics.

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Section: Introductionmentioning

confidence: 99%

Lymphatic response to depilation‐induced inflammation in mouse ear assessed with label‐free optical lymphangiography

2015

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“…Lymphoscintigraphy is a commonly-used imaging method in the clinic (Mihara et al, 2012; O'Mahony et al, 2004; Sevick-Muraca et al, 2014; Weissleder and Thrall, 1989; Wen et al, 2014) that relies on a radioactive tracer such as 99m -technetium ( 99m -Tc) being injected in the tissue (Kim et al, 2004b; Mieog et al, 2011; Ogasawara et al, 2008; Shih et al, 2001). As it drains through the lymphatic system, it can be imaged using a scintillation camera that integrates the signal over time to produce a 2D image of the lymphatic network (Fig.…”

Section: Imaging the Lymphatic System In The Clinic For Assessment Ofmentioning

confidence: 99%

Imaging the lymphatic system

Munn

Padera

2014

Microvascular Research

106

101

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Visualization of the lymphatic system is clinically necessary during diagnosis or treatment of many conditions and diseases; it is used for identifying and monitoring lymphedema, for detecting metastatic lesions during cancer staging and for locating lymphatic structures so they can be spared during surgical procedures. Imaging lymphatic anatomy and function also plays an important role in experimental studies of lymphatic development and function, where spatial resolution and accessibility are better. Here, we review technologies for visualizing and imaging the lymphatic system for clinical applications. We then describe the use of lymphatic imaging in experimental systems as well as some of the emerging technologies for improving these methodologies.

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Lymphoscintigraphy of melanoma: Lymphatic channel activity guides localization of sentinel lymph nodes, and gamma camera imaging/counting confirms presence of radiotracer in excised nodes

Cited by 2 publications

References 11 publications

Lymphatic response to depilation‐induced inflammation in mouse ear assessed with label‐free optical lymphangiography

Lymphatic response to depilation‐induced inflammation in mouse ear assessed with label‐free optical lymphangiography

Imaging the lymphatic system

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