Lymphoproliferative Diseases of Fowl: JM-V Leukemic Lymphoblasts in Cell Culture: Brief Communication2

Abstract

JM-V leukemic lymphoblasts were established in cell culture. The cultured cells (JM-VLC cells) were transplantable in young chicks and produced a disease indistinguishable from JM-V lymphoblastic leukemia as initiated by whole-blood inoculation. JM-VLC cells maintained a normal female karyotype through 13 passages in Rhode Island Red cockerels. With the use of JM-V antisera and antisera from birds with naturally occurring Marek's disease (MD), specific antigens were detected on the surfaces of living cells. Intracellular antigens were detected with anti-MD virus sera after cultivation for at least 1 day at 37 degrees C. In spite of the expression of MD antigens, the presence of herpesvirus particles associated with the cultured cells, and the occurrence of foci of multinucleated cells in kidney cultures from chicks inoculated with cellfree preparations of JM-VLC cells, the pathologic potential of the cultured cells was that of JM-V leukemia.

A nonimmunosuppressive helper virus allows high efficiency induction of B cell lymphomas by reticuloendotheliosis virus strain T.

“…Cell lines were isolated by preparing single-cell suspensions from nodules in the liver and random sections of tissue from the spleen, thymus, and bursa . These suspensions were diluted into Hahn's medium (22) and cultured at 37°C with 10% C02 for 48 h before transferring cultures at a 1 :5 dilution into ET,OCa, oCk2. Spleen and liver cultures were transferred at a 1 :10 dilution every 24-48 h thereafter.…”

The inhibitory effects of oligonucleotides, complementary to Marek's disease virus mRNA transcribed from the BamHI-H region, on the proliferation of transformed lymphoblastoid cells, MDCC-MSB1

“…A variety of transformed lymphoblastoid cell lines which contain multiple copies of the MDV genome have been established from MDV-induced lymphomas or transplantable lymphomas in chickens (Akiyama et al, 1974;Hahn et al, 1977). There was no amplification of the 132 bp repeat sequence within the BamHI-D and -H regions of latent viral DNA in the transformed lymphoblastoid cells (Hayashi et al, 1988) and the 1-8 kb transcript was produced from the BamHI-D and -H regions of MDV DNA in the transformed cells (Bradley et al, 1989a).…”

Duck lymphocytes. VIII. T-lymphoblastoid cell lines from reticuloendotheliosis virus-induced tumours

“…The most commonly successful medium was RPMI containing 5% PDS and 5% FCS, while RPMI supplemented with 5 to 15% PDS alone also supported the initial establishment of more cell lines than did RPMI supplemented with FCS alone. With the establishment of chicken cell lines transformed by Marek' s disease virus, several components of the Hahn medium cocktail (Hahn et al, 1977) were bene® cial, but 2-ME was the most critical (Calnek et al, 1978); no comparable requirements were observed with duck cells and, indeed, 2-ME had an inhibitory or toxic effect on duck cells. Following establishment and a period of expansion in the primary medium, the duck cell lines were readily weaned into FCS.…”

“…Supplements included: a pool of normal adult duck serum (PDS); foetal calf serum (FCS; Gibco or HyClone, Logan, Utah); 2-mercaptoethanol (2-ME); sodium pyruvate (SP); and tryptose phosphate broth (TPB). Modi® ed Hahn (M H) medium (Hahn et al, 1977) was RPMI containing 8% FCS, 10% PDS, 5% TPB, 1 mM SP and 10 mM 2-ME. Phytohaemagglutinin (PHA) and E. coli lipopolysaccharide (LPS) (both from Sigma, St Louis, Mo) were dissolved in RPMI and used at ® nal concentrations of 5 and 1 m g/ml, respectively.…”