Lymphoma in a Domestic Guinea Pig

Kołodziejska, A.; Depta, A; Szweda, M.

doi:10.1016/j.jcpa.2010.09.023

Cited by 2 publications

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“…Lymphoid neoplasia is infrequently reported in guinea pigs relative to other veterinary species; this may be due in part to an enzyme with L‐asparaginase activity discovered in guinea pig serum . However, disseminated/multicentric, ocular, and epitheliotropic lymphomas, as well as lymphocytic leukemia have been well‐described in guinea pigs. Guinea pigs possess an endogenous type C retrovirus, which has been associated with leukemia and exogenous transmission in an inbred strain of guinea pigs, but spontaneous viral‐induced leukemia has not been well‐established in a natural setting.…”

Section: Introductionmentioning

confidence: 99%

Validation of an immunocytochemical assay for immunophenotyping of lymphoma in guinea pigs (Cavia porcellus)

Harr

Thielen

MacNeill

2018

Veterinary Clinical Pathol

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Background: Lymphoma is an important disease of pet guinea pigs, although validation of immunophenotyping techniques based on cytologic or hematologic samples has not been reported. Objective:To describe an immunocytochemical method for immunophenotyping of lymphoma (as either T-or B-cell) in guinea pigs, and to validate antibodies for this purpose.Methods: Blood and tissues were obtained at the time of necropsy from laboratory guinea pigs and a privately owned dog (control) euthanized for reasons unrelated to lymphoproliferative disease. Fine-needle aspirates of enlarged peripheral lymph nodes were obtained from a case of spontaneous lymphoma in a pet guinea pig.Anti-CD3 and anti-Pax5 antibodies were validated by a combination of western blotting performed on splenic lysates of both the dog and guinea pigs, immunohistochemical studies on normal guinea pig tissues, and immunocytochemistry on normal guinea pig peripheral blood and splenic impression smears. Results:The antibodies bound to antigens of an appropriate size in both the dog and guinea pig splenic lysates by Western blot analysis. Immunohistochemistry and immunocytochemistry demonstrated the expected distribution of putative T-and Blymphocytes in normal tissues, peripheral blood, and splenic impression smears. As a proof-of-principle for its clinical utility, this immunocytochemical assay was used to diagnose a B-cell phenotype in a spontaneous lymphoma case in a pet guinea pig. Conclusions:Here, we validated an immunocytochemical method for immunophenotyping of lymphoma in guinea pigs as either a T-or B-cell phenotype. This enables future research into the clinical attributes of these subtypes and may ultimately improve both prognostication and therapy of lymphoma in guinea pigs.

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Section: Introductionmentioning

confidence: 99%

Validation of an immunocytochemical assay for immunophenotyping of lymphoma in guinea pigs (Cavia porcellus)

Harr

Thielen

MacNeill

2018

Veterinary Clinical Pathol

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Incidence of spontaneous tumours in guinea pigs: a retrospective study of 153 cases

Nešpor,

Heczková,

Škorič

2023

Acta Vet. Brno

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The aim of this study was to investigate the incidence of spontaneous tumours in guinea pigs in biopsy and necropsy samples in the monitored period (2016–2022) on a representative number of cases and to determine their occurrence in relation to age and sex of the animals. A total of 204 samples from various pathological lesions were examined, while 153 cases (75.00%) were true tumours and 51 cases (25.00%) were pseudotumorous non-neoplastic lesions. The distribution of the incidence of true neoplasms in relation to sex was uneven. In the examined group, more neoplasms were diagnosed in males (n = 88, 57.52%) compared to females (n = 65, 42.48%). The mean age of guinea pigs with diagnosed tumour in our group was 4.2 years. Thyroid adenocarcinoma was the most frequent tumour. The majority of thyroid tumours (67.86%) were diagnosed in animals aged 4–5 years, as a significant result in terms of incidence in this age range, as well as a predisposition to tumour formation in males (78.57%). Mammary gland tumours occurred in the age range of 2–7 years, with a mean age of incidence of 4.7 years. In all cases, these were malignant variants and showing a significant higher incidence in males, in the age range of 4–5 years. The processing of a representative number of samples and epidemiological data on the incidence of spontaneous tumours in guinea pigs can contribute to the overall expansion of knowledge about this topic in veterinary medicine.

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Lymphoma in a Domestic Guinea Pig

Cited by 2 publications

References 0 publications

Validation of an immunocytochemical assay for immunophenotyping of lymphoma in guinea pigs (Cavia porcellus)

Validation of an immunocytochemical assay for immunophenotyping of lymphoma in guinea pigs (Cavia porcellus)

Incidence of spontaneous tumours in guinea pigs: a retrospective study of 153 cases

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