Lymphoid Potential, Probed before Circulation in Mouse, Is Restricted to Caudal Intraembryonic Splanchnopleura

Cumano, Ana; Dieterlen‐Lièvre, Françoise; Godin, Isabelle

doi:10.1016/s0092-8674(00)80166-x

Cited by 524 publications

(326 citation statements)

References 34 publications

Supporting

Mentioning

308

Contrasting

Unclassified

Order By: Relevance

“…Most of the studies have found the simultaneous appearance of lymphoid progenitors in both yolk sac and embryo proper [58][59][60][61]. However, other studies have indicated that lymphoid potential arises within the embryo proper between E8.5 and E9.5 [62,63] and even earlier if the tissues are first explanted and expanded for several days on a bone marrow stromal cell line [64]. Other investigators have focused exclusively on either the yolk sac or the AGM region as the source of lymphoid potential [65,66].…”

Section: The Origin Of Lymphoid Precursors Remains Controversialmentioning

confidence: 99%

Yolk-sac hematopoiesis

Palis

Yöder

2001

Experimental Hematology

382

272

View full text Add to dashboard Cite

Objective. To review the process of blood-cell formation in the murine and human yolk sac. Data Sources. Most articles were selected from the PubMed database. Data Synthesis. The yolk sac is the first site of blood-cell production during murine and human ontogeny. Primitive erythroid cells originate in the yolk sac and complete their maturation, including enucleation, in the bloodstream. Though species differences exist, the pattern of hematopoietic progenitor cell emergence in the yolk sac is similar in mouse and man. In both species, there is a stage of development where both primitive red blood cells and definitive erythroid progenitors are produced in the yolk sac. An "embryonic" hematopoietic stem cell that engrafts in myeloablated newborn but not adult mice can be detected in the murine yolk sac and embryo. Stem-cell activity in the human yolk sac has not been reported. Conclusions. The yolk sac is the sole site of embryonic erythropoiesis. However, definitive erythroid, myeloid, and multipotential progenitors also originate in the yolk sac. The relationship between these progenitors and the "embryonic" hematopoietic stem cell has not been elucidated. Yolk sac-derived progenitor cells may seed the developing liver via the circulation and serve as the immediate source of the mature blood cells that are required to meet the metabolic needs of the rapidly growing fetus.

show abstract

Section: The Origin Of Lymphoid Precursors Remains Controversialmentioning

confidence: 99%

Yolk-sac hematopoiesis

Palis

Yöder

2001

Experimental Hematology

382

272

View full text Add to dashboard Cite

show abstract

“…4 Although multipotential hematopoietic progenitor cells revealed by in vitro colony assays, injection into yolk sac cavity, transplantation into fetal liver of newborn mice or reconstitution of immunodeficient mice, can be detected in the pSP/AGM region and yolk sac at earlier developmental stages, [12][13][14][15] the first definitive HSPCs, defined as cells fully competent to provide long-term reconstitution of irradiated adult recipients, emerge in the pSP/AGM region at embryonic day (E) 10.5. 6,9,11 Subsequently, at around E11.0, definitive HSPC activity can be found in yolk sac.…”

Section: Introductionmentioning

confidence: 99%

Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development

Jin

Wen

2007

Blood

146

151

View full text Add to dashboard Cite

The development of vertebrate definitive hematopoiesis is featured by temporally and spatially dynamic distribution of hematopoietic stem/progenitor cells (HSPCs). It is proposed that the migration of definitive HSPCs, at least in part, accounts for this unique characteristic; however, compelling in vivo lineage evidence is still lacking. Here we present an in vivo analysis to delineate the migration route of definitive HSPCs in the early zebrafish embryo. Cell-marking analysis was able to first map definitive HSPCs to the ventral wall of dorsal aorta (DA). These cells were subsequently found to migrate to a previously unappreciated organ, posterior blood island (PBI), located between the caudal artery and caudal vein, and finally populate the kidney, the adult hematopoietic organ. IntroductionThe ontogeny of vertebrate hematopoietic system is characterized by 2 phases of development: an early transitory primitive wave generating only primitive erythrocytes and some myeloid cells, and a definitive wave that initiates late and produces all the mature blood lineages (erythroid, myeloid, and lymphoid). [1][2][3][4] In vertebrates, the first hematopoietic activity can be identified in an extra-embryonic location: yolk sac in mice and chickens, or ventral blood island (VBI) in amphibians, leading to the hypothesis that yolk sac/VBI is the sole source of hematopoietic cells, which will in turn populate downstream hematopoietic compartments. 5 However, the singular hematopoietic origin from yolk sac/VBI is challenged by substantial evidence from experiments with various species that reveals another evolutionary conserved intraembryonic hematopoietic site: the para-aortic splanchnopleurae (pSP) and later referred as aorta-gonad-mesonephros (AGM). [6][7][8][9][10][11] Hence, it is currently believed that yolk sac/VBI mainly supports primitive hematopoiesis, whereas definitive hematopoiesis is autonomously initiated, at least in part, in the pSP/AGM region.The journey of definitive HSPCs is best characterized both temporally and spatially in mice. 4 Although multipotential hematopoietic progenitor cells revealed by in vitro colony assays, injection into yolk sac cavity, transplantation into fetal liver of newborn mice or reconstitution of immunodeficient mice, can be detected in the pSP/AGM region and yolk sac at earlier developmental stages, 12-15 the first definitive HSPCs, defined as cells fully competent to provide long-term reconstitution of irradiated adult recipients, emerge in the pSP/AGM region at embryonic day (E) 10.5. 6,9,11 Subsequently, at around E11.0, definitive HSPC activity can be found in yolk sac. 6,9,11 Recently, placenta is recognized as another embryonic reservoir from E10.5 for definitive HSPCs operation. 16,17 However, the cellular origin of definitive HSPCs in yolk sac and placenta and their contribution to adult hematopoiesis are not clear. From E11.5, fetal liver begins to be populated by definitive HSPCs and serves as the main site that supports hematopoietic expansion and differentiation dur...

show abstract

“…These intraembryonic HSCs appear in a cell-autonomous manner as cell clusters in the floor of the dorsal aorta and the vitelline and umbilical arteries. [17][18][19][20] HSCs first generate committed, transit-amplifying progenitors that then differentiate into hematopoietic lineage-restricted progenitor cells. 21 HSCs and progenitor cells migrate to colonize the fetal liver, the spleen, the thymus and finally the BM, which is the main hematopoietic organ during adult life.…”

mentioning

confidence: 99%

Notch1 regulates progenitor cell proliferation and differentiation during mouse yolk sac hematopoiesis

et al. 2014

View full text Add to dashboard Cite

Loss-of-function studies have demonstrated the essential role of Notch in definitive embryonic mouse hematopoiesis. We report here the consequences of Notch gain-of-function in mouse embryo hematopoiesis, achieved by constitutive expression of Notch1 intracellular domain (N1ICD) in angiopoietin receptor tyrosine kinase receptor-2 (Tie2)-derived enhanced green fluorescence protein (EGFP þ ) hematovascular progenitors. At E9.5, N1ICD expression led to the absence of the dorsal aorta hematopoietic clusters and of definitive hematopoiesis. The EGFP þ transient multipotent progenitors, purified from E9.5 to 10.5 Tie2-Cre;N1ICD yolk sac (YS) cells, had strongly reduced hematopoietic potential, whereas they had increased numbers of hemogenic endothelial cells. Late erythroid cell differentiation stages and mature myeloid cells (Gr1 þ , MPO þ ) were also strongly decreased. In contrast, EGFP þ erythro-myeloid progenitors, immature and intermediate differentiation stages of YS erythroid and myeloid cell lineages, were expanded. Tie2-Cre;N1ICD YS had reduced numbers of CD41 þ þ megakaryocytes, and these produced reduced below-normal numbers of immature colonies in vitro and their terminal differentiation was blocked. Cells from Tie2-Cre;N1ICD YS had a higher proliferation rate and lower apoptosis than wild-type (WT) YS cells. Quantitative gene expression analysis of FACS-purified EGFP þ YS progenitors revealed upregulation of Notch1-related genes and alterations in genes involved in hematopoietic differentiation. These results represent the first in vivo evidence of a role for Notch signaling in YS transient definitive hematopoiesis. Our results show that constitutive Notch1 activation in Tie2 þ cells hampers YS hematopoiesis of E9.5 embryos and demonstrate that Notch signaling regulates this process by balancing the proliferation and differentiation dynamics of lineage-restricted intermediate progenitors.

show abstract

Lymphoid Potential, Probed before Circulation in Mouse, Is Restricted to Caudal Intraembryonic Splanchnopleura

Cited by 524 publications

References 34 publications

Yolk-sac hematopoiesis

Yolk-sac hematopoiesis

Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development

Notch1 regulates progenitor cell proliferation and differentiation during mouse yolk sac hematopoiesis

Contact Info

Product

Resources

About