Lymphoid cell killing by human cytomegalovirus

Abstract

The interaction of human cytomegalovirus (CMV) with two replicating lymphoid cell lines, Ra-1 and MOLT-4F was studied. It was determined that while the cells exhibited viral receptors, the production of viral antigen and fully infectious virus could not be documented. Instead, cell death proportionate to the quantity of virus inoculated was seen. Neutralization of cell killing was effected with CMV-specific antiserum. In addition it was ascertained that cell death appeared to require a viral genomic function. This conclusion was based on the observation that UV irradiation of virus stocks reduced the cell killing capacity of the preparation. Infectivity of virus preparations was more sensitive to UV than the cell killing property. When compared on the basis of infectivity for fibroblasts and lymphoid cell killing capacity, pools of CMV with high infectivity had relatively less cell-killing capacity than low titered preparations.

Festschrift Immunological aspects of interaction between rotavirus and the intestine in infancy

“…The characteristics of the CMI response to rotavirus in suckling mice were studied in spleen lymphocytes from infected and uninfected animals using the microtitre lymphocyte transformation assay (40). The spleen cells were challenged with different concentrations of MRV antigen and mitogens.…”

“…To investigate the relative role of T and B cell immune response in rotavirus infection, we used mice with isolated T cell deficiency (congenitally athymic nude mice), combined B and T cell deficiency (severe combined immuno deficiency; SCID) and immunologically normal seronegative (control) suckling mice (30,42). The three groups of infant mice were orally inoculated with MRV and examined for intestinal virus replication in isolated enterocytes and assessed for diarrhoea and virus shedding.…”

“…Whether these alterations in the course of rotavirus infection in malnutrition are due to impaired immune response or changes in the gut morphology is not known. Studies in adult mice have demonstrated that malnutrition does not affect the state of refractoriness to symptomatic rotavirus infection (45).…”