Lymphocytic Choriomeningitis Virus-Induced Alterations of T Helper-Mediated Responses in Mice Developing Autoimmune Hemolytic Anemia During the Course of Infection

Idrissi, M. El Azami El; Mazza, Graziella; Monteyne, Philippe; Elson, C. J.; Day, M. J.; Pfau, C. J.; Coutelier, Jean‐Paul

doi:10.3181/00379727-218-44303

Cited by 7 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RT-PCR. mRNA was prepared from lysed adherent cells as reported previously (El Azami El Idrissi et al, 1998). CD14 gene expression was measured by RT-PCR with primers described by Liu et al (2006).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Modulation of lipopolysaccharide receptor expression by lactate dehydrogenase-elevating virus

Le-Thi-Phuong

Coutelier

2012

Journal of General Virology

View full text Add to dashboard Cite

Lactate dehydrogenase-elevating virus (LDV) exacerbates mouse susceptibility to endotoxin shock through enhanced tumour necrosis factor (TNF) production by macrophages exposed to lipopolysaccharide (LPS). However, the in vivo enhancement of TNF production in response to LPS induced by the virus largely exceeds that found in vitro with cells derived from infected animals. Infection was followed by a moderate increase of Toll-like receptor (TLR)-4/MD2, but not of membrane CD14 expression on peritoneal macrophages. Peritoneal macrophages from LDVinfected mice unresponsive to type I interferons (IFNs) did not show enhanced expression of TLR-4/MD2 nor of CD14, and did not produce more TNF in response to LPS than cells from infected normal counterparts, although the in vivo response of these animals to LPS was strongly enhanced. In contrast, the virus triggered a sharp increase of soluble CD14 and of LPS-binding protein serum levels in normal mice. However, production of these LPS soluble receptors was similar in LDV-infected type I IFN-receptor deficient mice and in their normal counterparts. Moreover, serum of LDV-infected mice that contained these soluble receptors had little effect if any on cell response to LPS. These results suggest that enhanced response of LDV-infected mice to LPS results mostly from mechanisms independent of LPS receptor expression.

show abstract

Section: Methodsmentioning

confidence: 99%

“…As an internal control, housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT) gene expression was measured using primers described by Peterson et al (1994). The ratios between CD14 and HPRT expression were calculated and expressed as arbitrary units (El Azami El Idrissi et al, 1998).…”

Section: Methodsmentioning

confidence: 99%

Modulation of lipopolysaccharide receptor expression by lactate dehydrogenase-elevating virus

Le-Thi-Phuong

Coutelier

2012

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…An early expression of IFN-c message, analysed as described previously (El Azami El Idrissi et al, 1998), was found in 129/Sv mice, with the strongest message at 2 days after infection (Fig. 1c).…”

mentioning

confidence: 87%

“…T lymphocytes were depleted with GK1.5 anti-CD4 (Dialynas et al, 1983) or 53/6.72 anti-CD8 monoclonal antibody, obtained from ATCC (Ledbetter & Herzenberg, 1979). In vivo efficiency of these antibodies has been extensively reported previously (Coulie et al, 1985;Coutelier, 1991;El Azami El Idrissi et al, 1998). This treatment was not followed by modification in IFN-c levels (Fig.…”

mentioning

confidence: 99%

Production of protective gamma interferon by natural killer cells during early mouse hepatitis virus infection

Thirion

Coutelier

2009

Journal of General Virology

View full text Add to dashboard Cite

Gamma interferon (IFN-γ) plays a major role in the protection against lethal infection with mouse hepatitis virus A59. IFN-γ production reaches a maximum level 2 days after viral inoculation, especially in liver immune cells. Among these cells, natural killer cells are the major producers of this cytokine. Transfer experiments indicated that the protective role of IFN-γ is mediated through a direct effect on cells targeted by the virus rather than through indirect activation of T lymphocytes.

show abstract

“…Gene expression was analyzed by RT-PCR as described previously (44). Cells were lysed in Trizol reagent (BRL, Gaithersburg, MD).…”

Section: Rna Extraction and Pcr Amplificationmentioning

confidence: 99%

Distinct requirements for IL-6 in polyclonal and specific Ig production induced by microorganisms

Markine-Goriaynoff¹,

Nguyễn²,

Bigaignon³

et al. 2001

International Immunology

View full text Add to dashboard Cite

The role of IL-6 in Ig production induced in the mouse by lactate dehydrogenase-elevating virus (LDV), Toxoplasma gondii or lipopolysaccharide (LPS) was assessed. Following infection with LDV, a strong activator of B cells, an early and transient IL-6 production was observed, that originated predominantly from macrophages. Whereas LDV-induced B lymphocyte proliferation appeared independent of IL-6, mice deficient for this cytokine showed a marked reduction in their total T-dependent IgG2a production when compared to their normal counterparts. By contrast, specific responses directed against either LDV or non-viral antigens administered at the time of infection were not decreased in the absence of IL-6. Similarly, polyclonal, but not anti-parasite IgG2a production triggered by T. gondii infection was strongly dependent on the presence of IL-6. Finally, T-independent total IgG3 secretion triggered by LPS was also markedly reduced in IL-6-deficient mice. These results suggest that IL-6 plays a major role in T-dependent and T-independent polyclonal Ig production following B lymphocyte activation by viruses, and parasites, but not in specific antibody responses induced by the same microorganisms.

show abstract

Lymphocytic Choriomeningitis Virus-Induced Alterations of T Helper-Mediated Responses in Mice Developing Autoimmune Hemolytic Anemia During the Course of Infection

Cited by 7 publications

References 16 publications

Modulation of lipopolysaccharide receptor expression by lactate dehydrogenase-elevating virus

Modulation of lipopolysaccharide receptor expression by lactate dehydrogenase-elevating virus

Production of protective gamma interferon by natural killer cells during early mouse hepatitis virus infection

Distinct requirements for IL-6 in polyclonal and specific Ig production induced by microorganisms

Contact Info

Product

Resources

About