Lymphocytes in Peyer patches regulate clinical tolerance in a murine model of food allergy☆

Frossard, C; Tropia, Laurence; Hauser, Conrad; Eigenmann, Philippe

doi:10.1016/j.jaci.2003.12.017

Cited by 69 publications

(66 citation statements)

References 23 publications

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“…Several studies correlated oral tolerance with T regulatory cells (T reg) or clonal anergy/deletion, respectively, in response to low or high doses of fed antigens (2). In particular, CD4+CD25+ regulatory T cell clones have resulted inducible by the antigen in Peyer's patches of animal models (3). Since a natural outgrowth of cow's milk allergy has been associated with high blood levels of CD4+CD25+Foxp3+ T (reg) in children (4), we studied the role of this cell subset in ten selected children, before.…”

mentioning

confidence: 99%

CD4+CD25+Foxp3+ T Regulatory Cells are not Involved in Oral Desensitization

Mori

Bianchi

Pucci

et al. 2010

Int J Immunopathol Pharmacol

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Oral tolerance has been related to generation of T regulatory cells (Treg) or clonal anergy/deletion, respectively by administering low and high doses of fed antigens. CD4+CD25+ regulatory T cell clones can be induced by the antigen in Peyer's patches of animal models. We selected ten subjects (mean age: 89.4 ± 36.21 months; group A) with severe cow's milk allergy. They underwent oral desensitization (OD) according to the current protocols. In six months they reached a tolerance of 50 ml of cow's milk. CD4+CD25+Foxp3+ T(reg) blood levels were measured at the beginning of OD (A) and after 6 months (A'), but almost the same values were obtained: A = 0.36 ± 0.11%; A'= 0.59 ± 0.15%. These results were compared with a control group (C) of non-atopic children. Naturally outgrowing cow's milk allergy can be related to high blood levels of CD4+CD25+Foxp3+ T(reg), as previously reported in children. On the other hand, a forced oral desensitization through a progressive intake of the antigenic food seems not to be related to an enhancement of CD4+CD25+Foxp3+ T(reg) levels in peripheral blood, making the role of long-lasting systemic immunologic changes unlikely.

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mentioning

confidence: 99%

CD4+CD25+Foxp3+ T Regulatory Cells are not Involved in Oral Desensitization

Mori

Bianchi

Pucci

et al. 2010

Int J Immunopathol Pharmacol

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“…PP is found in sheep, calves, swine and horses in the ileum, while in the appendix in rabbits, and bursa in chickens 15 . Recent studies have indicated an interest in PP as an immune response mediator 15,16,17 , without giving importance to the distinct characteristics of the colonic tissue in evaluating the healing processes that take place on this structure. In the indexed literature, one single study makes mention of the collagen in this region 18 .…”

Section: Discussionmentioning

confidence: 99%

“…This observation reinforces the importance of experimental studies evaluating the healing of anastomoses in the left colon, especially studies using animals of only a single gender. Studies have habitually used animals of male gender [1][2][3][4][6][7][8][10][11][12][13]16,18 . even though there is no restriction on using females, purely from the point of view of location of the PP 5,14,21 .…”

Section: Discussionmentioning

confidence: 99%

“…This use of PP as an anatomical repair landmark reduces the risks of anastomoses performed on colonic tissue in terms of differences in compositional, histological, biochemical and functional characteristics 16,18,19,20 . Uniformity of the tissue chosen ensures precision in studies relating to the healing of anastomoses and their resistance to pressure and tension tests, and in biochemical and histological studies that qualitatively and quantitatively analyze the collagen content in surgical anastomoses.…”

Section: Discussionmentioning

confidence: 99%

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Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

et al. 2009

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PURPOSE: To validate Peyer's patch as an anatomical repair landmark for colorectal surgery in rats and to measure the collagen content in segments of the colon containing or not containing Peyer's patch. METHODS: The distance between Peyer's patch and the peritoneal reflection was measured in forty-five Wistar rats. The colon and rectum were resected for quantification of collagen content by means of computer-assisted image analysis in regions of the colon with and without Peyer's patch. RESULTS: There was great variation in the distance between Peyer's patch and the peritoneal reflection when the male and female rats were considered as a single group (p=0.04). Comparison between the genders showed that the distance between the patch and the peritoneal reflection was greater in female than in male rats (p=0.001). The colonic segment containing Peyer's patch was observed to have lower tissue collagen content than the segment in which this structure was not present (p=0.02). CONCLUSION: Peyer's patch can be indicated as an anatomical repair landmark, and there is a need to study the healing of colorectal anastomoses in rats based on differing quantities of tissue collagen existing in the colonic wall with or without this structure.

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“…Our results showed clearly the development of hypersensitivity against the antigen administered (α-Lac). Frossaed et al [16] showed that the anaphylactic mice immunized with α-Lac exhibit a sharp increase in antibody titers IgG1 anti α-Lac and an increase in production of IL4. Our results showed that the ingestion of coconut milk leads to a significant decrease in the systemic immune response, as demonstrated by the low level of production of IgG antibodies to α-Lac.…”

Section: Evaluation Of Intestinal Villous Height Of Mice Fed With Cocmentioning

confidence: 99%

Coconut Milk Modulate the Antigenicity of Alpha-Lactalbumin in Balb/C Mice

Benaissa

Addou

Dib

et al. 2017

Int J Pharm Pharm Sci

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Objective: The aim of this work was to study the biochemical characteristics of coconut milk and its antigenic effect on the Balb/c mice immunized with α-lactalbumin protein, as well as its consequences on the structure of the intestinal epithelium.Methods: To achieve the objective of the study, an electrophoresis was realised on a polyacrylamide gel to determine various proteins contained in coconut milk. In addition, Lowry's method was used to determine the amount of proteins in the formula. The antigenicity of coconut milk in sera was also studied using the Enzyme-Linked Immunosorbent Assay (ELISA) method. For the histological study, 21 w-old mice Balb/c were used and distributed in three groups of 7 mice each. Group 1, received a standard feed with no treatment (Negative control), group 2 and 3 received respectively a standard feed (Positive control) and coconut milk for a period of 28 d after being immunized with α-lactalbumin.Results: Analysis of the data revealed that the rate of proteins of cow's milk is higher than that of the coconut milk (0.001 ≤ p ≤ 0.01). However, after carrying out the electrophoresis analysis, the coconut milk showed the absence of intact proteins. The anti α-Lactalbumin IgG titers significantly increased in positive control groups that received coconut milk (p<0.0001). Moreover, there was an increase of the intestinal villi height of mice fed with coconut milk, in the structure level of their intestinal epithelium compared to the negative control group. Conclusion:The findings of the study provide the evidence that coconut milk is a possible alternative to the cow's milk formula in case of allergy.

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Lymphocytes in Peyer patches regulate clinical tolerance in a murine model of food allergy☆

Cited by 69 publications

References 23 publications

CD4+CD25+Foxp3+ T Regulatory Cells are not Involved in Oral Desensitization

CD4+CD25+Foxp3+ T Regulatory Cells are not Involved in Oral Desensitization

Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

Coconut Milk Modulate the Antigenicity of Alpha-Lactalbumin in Balb/C Mice

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