Lymphocytes: Circulation Altered by Trypsin

Abstract

Rat thoracic duct lymphocytes altered by trypsin in vitro do not circulate normally. At early intervals after transfusion of lymphocytes labeled with chromium-51 selective accumulation of radioactivity in the lymph nodes is abolished, while uptake in the spleen is not reduced. Later, the cells appear to "home" to lymph nodes and recirculate to the lymph.

Vitamin A Deficiency and Immunity.

“…In rats, for example, the redistribution of some lymphocyte subpopulations could deplete splenic lymphocytes which respond to ConA, PHA or LPS and reveal the existence of a suppressor T lymphocyte population responsible for the inhibited blastogenic response [51]. Alteration of membrane lymphocyte receptor synthesis and redistribution of lymphocyte populations are not mutually exclusive hypotheses: the former has consequences in terms of the latter [117][118][119]. Numerous authors support the alternative hypothesis of a vitamin A effect on lysosomal membranes [77,[121][122][123]: the enzymes would be delivered in the presence of an excess of vitamin A [123][124][125][126][127][128][129][130][131].…”

“…It has been chemically proven that these glycoproteins cover T-and B-lymphocyte membranes [120]. Lymphocyte treatment with a glycosidase, trypsin or neuraminidase before intravenous transfusion significantly alters the normal mode of circulation of these cells [117][118][119]. In rats, for example, the redistribution of some lymphocyte subpopulations could deplete splenic lymphocytes which respond to ConA, PHA or LPS and reveal the existence of a suppressor T lymphocyte population responsible for the inhibited blastogenic response [51].…”

“…These proteins are constituents of cell membranes and participate in several functions that may be altered during vitamin A deficiency [69,[113][114][115][116]. The hypothesis for the mode of action of vitamin A is based on this observation: glycoproteins represent important cellular receptors for numerous biological effectors, in particular mitogens and recognition molecules that determine lymphocyte distribution in lymph nodes and spleen [117][118][119]. It has been chemically proven that these glycoproteins cover T-and B-lymphocyte membranes [120].…”

Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells

“…Fidler (1978b) reported that treatment of cultured B16 melanoma cells with trypsin-EDTA mixture for increasing lengths of time successively decreased their pulmonary colonisation capacity. It has also been found that alterations of the cell surface properties of non-neoplastic cells (normal adult lymphocytes) by enzymes (Gesner & Ginsburg, 1964;Woodruff & Gesner, 1968, 1969Woodruff, 1974) and by lectins (Schlesinger & Israel, 1974) altered their migratory patterns and homing properties after release into the circulation.…”

Surface Carbohydrate Composition of Different Types of Chicken Lymphocytes

“…Gesner and collaborators have shown that the migration patterns of rat thoracic duct lymphocytes were significantly changed after treatment with glycosidases [ 5 ] , trypsin [6], and neuraminidase [7]. This led to the concept that the migration pathways of lymphocytes are determined by specific recognition sites, e.g.…”