Lymphocytes and T lymphocyte subsets are regionally distributed in the female goat reproductive tract: influence of the stage of the oestrous cycle

Pérez-Martínez, Mario; Luna, J.; Mena, Raúl; Romano, M.C.

doi:10.1053/rvsc.2001.0532

Cited by 12 publications

(11 citation statements)

References 41 publications

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“…However, the role of embryo in modulating the differentiation and secretive activity of uterine leukocytes in ruminants remains unclear. In addition, previous study showed that the numbers of lymphocytes in the goat endometrium are influenced by the hormonal environment during early pregnancy [ 8 ]. An inadequate uterine environment induced by insufficient steroid concentration during early pregnancy in ewes can cause early embryonic death [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Goat Uterine DBA+ Leukocytes Differentiation and Cytokines Expression Respond Differently to Cloned versus Fertilized Embryos

et al. 2015

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High rate of fetal mortality in ruminant somatic cell nuclear transfer (SCNT) pregnancies is due, at least in part, to immune-mediated abortion of fetuses. In the present study, goat uterine leukocytes were isolated by Dolichos biflorus agglutinin (DBA) coated magnetic beads, and with majority being were CD56+CD16- in phenotype with low levels of perforin and Granzyme B expression. The responses of the isolated cells to SCNT and in vitro fertilization (IVF) embryos conditioned mediums containing hormone steroids were compared by measuring their phenotype and cytokines expression. The results showed there was a 2-fold increase in the numbers of isolated uterine leukocytes after incubation with different conditioned mediums for 120 h. However, significantly lower percentage and absolute numbers of uterine CD56+CD16- leukocytes incubated with SCNT conditioned mediums were detected as compared with those incubated with IVF conditioned mediums (P < 0.05). The group treated with progesterone (P4) or the combination of P4 and 17β-estradiol (E2) were associated with significantly higher percentage and absolute numbers of CD56+CD16- cells as compared with those treated with E2 alone (P < 0.05). Furthermore, in the presence of steroids, the isolated leukocytes incubated with SCNT conditioned mediums associated with greater levels of IFN-γ secretion and expression, as well as lesser levels of VEGF, as compared with those treated with IVF conditioned mediums (P < 0.05). In conclusion, this study demonstrates that SCNT embryos have a profound effect on the phenotype expression of goat uterine DBA+ leukocytes, as well as the secretion and expression of IFN-γ and VEGF by these cells in vitro.

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Section: Introductionmentioning

confidence: 99%

Goat Uterine DBA+ Leukocytes Differentiation and Cytokines Expression Respond Differently to Cloned versus Fertilized Embryos

et al. 2015

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“…types and number of immune cells at or near implantation sites. Although exact mechanisms by which the maternal immune environments are established during the period of early pregnancy are not fully understood, it is likely that changes in the distribution of immune cells 3 and patterns of local cytokine productions are involved in the establishment of proper microenvironments in utero 4–7 …”

Section: Introductionmentioning

confidence: 99%

Changes in Immune Cell Distribution and IL‐10 Production are Regulated through Endometrial IP‐10 Expression in the Goat Uterus

Imakawa

Nagaoka

Nojima

et al. 2005

American J Rep Immunol

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“…Further more, confirmed by morphological and histological evaluation of the ovary and endometrial layers of reproductive tract. Morphologically, appearance of ovarian follicles >5 mm refers to follicular phase or rising of the mature corpus luteum intimate to luteal phase of ewe's estrous cycle [18] . Histologically, straight and narrow tubular glands of the endometrium were observed during follicular phase, but the presence of the corpus luteum with typical secretory endometrium and the uterine glands begin secretion, dilating and becoming coiled were observed during luteal phase [1] .…”

Section: Animalsmentioning

confidence: 99%

“…The tissues were processed for paraffin embedding and preparations of the sections were done according to histological protocol. Methyl Green-Pyronine (Sigma, USA) staining was used to detect of plasma cells in the tissue sections [18] .…”

Section: Collection and Staining Of Tissuesmentioning

confidence: 99%

Secretary IgA Concentrations and plasma Cell Count Changes Associated with the Estrous Cycle in Ewes

Noordin¹

2009

American J. of Animal and Veterinary Sciences

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Problems statement:The level of uterine Secretory-IgA (S-IgA) and numbers of plasma cells was measured to observe the differences between two stages of estrous cycle (follicular and luteal phase) in the healthy cycling non pregnant ewes. Approach: Twelve ewes were used in this study and they were divided into two groups of 6 animals each according to the stages of estrous cycle. All ewes were subjected to estrous synchronization and allowed to undergo one natural estrous cycle after the removal of the sponge. All animals were then slaughtered at the end of the experiment. The uterine mucus was collected by flushing with a mixture of protease inhibitor cocktail in distilled water. For both stages, the level of uterine S-IgA was quantified by using ELISA and Methyl Green Pyronine staining was used to observe the plasma cell in the tissues of the uterine horn and oviduct of ewe's genital tract. Results: The results were analyzed by independent sample t-test and presented as mean±SEM. This study showed the relationship of the estrous cycle stages to uterine S-IgA concentration (µg mL −1 ) and populations of plasma cell in the healthy non-pregnant cycling ewes. The concentration (µg mL −1 ) of S-IgA (0.20±0.01) in the follicular phase was highly significant (p<0.01) as compared with the luteal phase (0.17±0.002). In addition, the populations of the plasma cells were significantly higher (p<0.01) in the uterine horn (4.97±0.32) and oviduct (3.82±0.33) during follicular phase compared to the luteal phase (3.87 ±0.30) and (1.90±0.21), respectively. Conclusion: The main reason for the immunosuppression during the luteal phase did not fully justified, especially with the presence of potential acquired infection during coitus in the follicular phase and at the same time immune system should decrease accordingly to prevent newly attached fetus rejection by the mother immune system.

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Lymphocytes and T lymphocyte subsets are regionally distributed in the female goat reproductive tract: influence of the stage of the oestrous cycle

Cited by 12 publications

References 41 publications

Goat Uterine DBA+ Leukocytes Differentiation and Cytokines Expression Respond Differently to Cloned versus Fertilized Embryos

Goat Uterine DBA+ Leukocytes Differentiation and Cytokines Expression Respond Differently to Cloned versus Fertilized Embryos

Changes in Immune Cell Distribution and IL‐10 Production are Regulated through Endometrial IP‐10 Expression in the Goat Uterus

Secretary IgA Concentrations and plasma Cell Count Changes Associated with the Estrous Cycle in Ewes

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