Lymphocyte subsets in the peripheral blood of patients with multiple myeloma and benign monoclonal gammopathy

Herrmann, F; Lochner, Adriane; Jauer, B.; Sieber, G.; Rühl, H.

doi:10.1007/bf01496728

Cited by 12 publications

(10 citation statements)

References 14 publications

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“…Therefore, the OKT4/OKT8 ratio was significantly elevated (Table 3). These results differ from those published so far [9,13,25]. Mellstedt et al [13] reported decreased percentages of OKT4 ÷ lymphocytes both in MM and in BMG with unaltered OKT4/OKT8 ratios.…”

Section: Discussioncontrasting

confidence: 80%

“…DR + lymphocytes of untreated and treated MM were within the normal range, whereas sIg ÷ cells revealed a significant decrease in both groups (P < 0.05), as previously described by Dillman et al [6]. In contrast, there are other reports of normal or elevated B cell counts in MM [9,13,25].…”

Section: Discussionsupporting

confidence: 65%

“…Mellstedt et al [13] reported decreased percentages of OKT4 ÷ lymphocytes both in MM and in BMG with unaltered OKT4/OKT8 ratios. The OKT4/OKT8 ratio was described as normal in BMG, with a nearly normal percentage of OKT4 ÷ cells [9]. In MM, however, a decreased percentage of OKT4 ÷ lymphocytes with a reduced OKT4/OKT8 ratio was detected [9].…”

Section: Discussionmentioning

confidence: 97%

“…The OKT4/OKT8 ratio was described as normal in BMG, with a nearly normal percentage of OKT4 ÷ cells [9]. In MM, however, a decreased percentage of OKT4 ÷ lymphocytes with a reduced OKT4/OKT8 ratio was detected [9]. The enumeration of helper and suppresor lymphocytes utilizing Fc receptors revealed a highly reduced TM population in both MM and BMG, with a concomitant decrease of the TM/T G ratio [25].…”

Section: Discussionmentioning

confidence: 99%

“…DR + lymphocytes were enumerated by immunofluorescent staining with heterologous antisera (alpha-gamma Lab., Sierra Madre, Calif) according to Kadin and Billing [9]. Spontaneous rosette formation (E rosettes) with sheep red blood cells (SRBC) was tested with neuraminidase-pretreated SRBC [8].…”

Section: Methodsmentioning

confidence: 99%

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Imbalances of T-cell subsets in monoclonal gammopathies

Bergmann

Mitrou

Weber

et al. 1984

Cancer Immunol Immunother

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In 42 patients with untreated or treated multiple myeloma (MM) or benign monoclonal gammopathy (BMG) the lymphocytes and T lymphocyte subsets were determined by monoclonal antibodies and other surface markers. In untreated MM, the T cells (1077/microliters vs 1439/microliters, P less than 0.01) and especially the OKT4+ lymphocytes (700/microliters vs 950/microliters, P less than 0.05) were significantly reduced compared with a control group. The OKT8+ cells were slightly but not significantly decreased. In previously treated MM, the loss of T cells was more pronounced than in the untreated group and was primarily caused by a further reduction of OKT4+ cells. Patients with BMG revealed decreased OKT8+ lymphocytes (304/microliters vs 502/microliters, P less than 0.001), whereas the OKT4+ cells were within the normal range. Therefore, the OKT4/OKT8 ratio was significantly elevated compared with that in untreated MM patients and normal controls (3.31 vs 2.06 vs 2.13; P less than 0.005). To sum up, in MM the results revealed a reduction of T cells, mainly of OKT4+ cells, which is intensified by chemotherapy and persists even after a long therapy-free interval. The different findings of T cell subsets in BMG and MM may be a helpful criterion to differentiate between BMG and MM.

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Section: Discussioncontrasting

confidence: 80%

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Imbalances of T-cell subsets in monoclonal gammopathies

Bergmann

Mitrou

Weber

et al. 1984

Cancer Immunol Immunother

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Selective loss of CD4+ CD45R+ T cells in peripheral blood of multiple myeloma patients

et al. 1988

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The phenotypic distribution of T-lymphocyte subsets in peripheral blood from multiple myeloma (MM) patients shows a reduced proportion of CD4+ cells and a normal proportion of CD8+ cells. The decrease in CD4+ cells could be due to a random process, with all types of CD4+ cells being equally affected, or it could reflect a nonrandom process with selected subsets preferentially reduced. In order to distinguish between these possibilities, double immunofluorescence analysis was performed on blood samples from patients with MM, patients with monoclonal gammopathy of unknown significance (MGUS), and age-matched normal donors, using monoclonal anti-CD4 or anti-CD8 paired with antibodies to the common leukocyte marker Lp220 (CD45R) or 4B4 (CDw29). Normal peripheral blood lymphocytes (PBL) include two phenotypically and functionally distinct CD4+-cell subsets, identified as CD4+ Lp220+ 4B4- and CD4+ Lp220-4B4+, whereas the majority of CD8+ cells expresses Lp220 (70-85%). MM patients had a highly significant selective reduction of the CD4+ Lp220+ subset compared with normal controls (P less than 0.001). Although the percentage of CD4+ Lp220- cells was also reduced in some MM patients relative to normal donors, most of MM patients had an elevated Lp220-/Lp220+ ratio of CD4+ cells (P less than 0.001). The proportion of the two CD8+ subsets was also markedly abnormal. In the set of patients studied the abnormalities within the CD4+ and CD8+ lymphocytes were exclusive to patients with MM since patients with MGUS had normal proportion of CD4+ and CD8+ subsets.

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