Lymphocyte subsets in porcine tonsillar crypt epithelium

Salles, Mônica Weissmann Seabra; Middleton, Dorothy M.

doi:10.1016/s0165-2427(00)00231-2

Cited by 19 publications

(12 citation statements)

References 34 publications

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“…Tonsils are known to be the sites of multiplication for many microorganisms, including Salmonella spp. (Fedorka-Cray et al, 2000;Salles & Middleton, 2000), and the oral cavity has been recently reported as an organic location where Salmonella spp. can be isolated more frequently than from faeces (Van Damme, Mattheus, Bertrand, & De Zutter, 2018).…”

Section: Discussionmentioning

confidence: 99%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

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Summary The importance of wild boars as game species in Spain is well known. Their feeding habits and intrusive behaviour, together with a progressively wider spreading of populations, increases the interactions of these animals with livestock and humans. Considering that wild boars could have a potential role in the transmission of certain pathogens as salmonellae, the aims of this study were to determine the prevalence of Salmonella spp. in wild boars hunted in central‐western Spain, the occurrence of this pathogen in tonsils, mandibular lymph nodes and faeces (as markers for transmission risk), and to define the phylogenetic relationships among isolated strains, in order to investigate the circulation pathways of bacteria among tissues, animals and estates. Samples from 1,041 hunted wild boars were analysed for the presence of Salmonella spp. by bacteriological culture. Isolates were confirmed by PCR and serotyped in the Spanish national reference laboratory. The genetic relationships between strains were determined by PFGE. The results showed a 7.7% of positive animals (81 wild boars), being tonsils the organ most frequently colonised by Salmonella spp. (18.7%), followed by lymph nodes (5.1%) and faecal samples (2.9%). Serovars Enteritidis and Newport were the most frequent amongst the 34 different serovars obtained. The pulsed‐field gel electrophoresis (PGFE) analysis showed a great genetic diversity, with serovars that exhibited different pulsotypes when isolated from different estates and multiple serovars in the same estate. In conclusion, this study reveals the importance of wild boars as carriers and possible transmitters of virulent and/or antimicrobial‐resistant clones of Salmonella spp. to livestock and humans.

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Section: Discussionmentioning

confidence: 99%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

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“…Alternatively, it may be a specific adaptation to facilitate rapid antigen uptake and T cell activation in appropriate conditions in a species in which the normal conjunctiva contains few lymphocytes and/or in a mucosa where the epithelium is normally stratified, bears tight junctions in the apical layers and, is relatively impermeable. 18 37 It is reminiscent of reticulosis of tonsils during acute tonsillitis 38 and of the extreme thinning and loss of epithelial basement membrane observed in organised human CALT at the point of maximal leucocytic aggregation. 13 15 It is suggested that breakdown of epithelial basement membrane occurs in MALT in areas of leucocytic infiltration to facilitate antigen transport.…”

Section: Discussionmentioning

confidence: 99%

Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats

Banerjee

Figueiredo²,

Easty³

et al. 2003

British Journal of Ophthalmology

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Aim: To investigate the development of lymphoid aggregates in the conjunctiva after corneal transplantation in rats. Methods: LEW or PVG strain corneas were transplanted orthotopically to PVG rats. Cornea and conjunctiva were examined clinically for up to 42 days. Eyes were removed with attached conjunctiva on days 10 and 15 after transplantation (before and during rejection), together with normal eyes, fixed, paraffin embedded, and examined immunohistochemically. Results: Clinically, the temporal half of the upper palpebral conjunctiva of recipients of 10/19 allografts and 1/10 isografts developed pronounced swelling, correlating with inflammation and rejection. Histologically, the swelling comprised leucocytic aggregates with an altered overlying epithelium. Aggregates contained granulocytes, macrophages, and cells expressing major histocompatibility complex (MHC) class II, CD4, and CD8, all more numerous in allograft associated conjunctiva. Class II + cells were more abundant at the surface, whereas macrophages and T cells were more numerous in the deeper stroma. There were few B cells. There was greater CD54 expression by vascular endothelium in allograft associated aggregates. Cells expressing TNFa and IFNc but not IL1b were present in stromal and superficial areas. Conclusions: Corneal transplantation in rats induces the development of organised conjunctival leucocytic aggregates in a fixed location that are significantly more pronounced in recipients of allografts compared with isografts and show characteristics of a Th1 type immune response. These aggregates have characteristics of conjunctiva associated lymphoid tissue and may be sites of presentation of graft antigens and lymphocyte proliferation at the ocular surface.

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“…Patches of LE characterized by high numbers of intraepithelial lymphocytes within the layer of non-keratinized epithelial cells, M cells and goblet cells are present within crypts [13]. The frequency and size of LE varies among and within animals [111]. The intraepithelial lymphocytes in the LE are B and T lymphocytes.…”

Section: Tonsils Of the Soft Palatementioning

confidence: 99%

“…The intraepithelial lymphocytes in the LE are B and T lymphocytes. Within the T lymphocyte subset, CD4 + T lymphocytes are the most frequent followed by γδ and CD8 + T lymphocytes [111]. M cells are not uniform, but have variable surface morphology [13].…”

Section: Tonsils Of the Soft Palatementioning

confidence: 99%

MALT structure and function in farm animals

2006

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-Mucosa-associated lymphoid tissue (MALT) is defined as an organized lymphoid tissue in the mucosa that samples antigens. The morphological characteristics that distinguish MALT from lymphoid infiltrates are discussed. MALT has been extensively investigated in laboratory animals, while knowledge in cattle, sheep, goats, pigs and horses that are summarized under the term farm animals in this review is fragmentary. Literature data about the distribution, morphology, function and involvement in infectious diseases of MALT in farm animals are described. The understanding of specific features of MALT in other species than laboratory animals is important for comparative research, in order to understand pathological and immunological processes in the respective species and as a potential route of vaccination of mucosal surfaces.

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Lymphocyte subsets in porcine tonsillar crypt epithelium

Cited by 19 publications

References 34 publications

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats

MALT structure and function in farm animals

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