Lymphocyte production of gamma-interferon as a test for non-tuberculous mycobacterial lymphadenitis in childhood

Davidson, Patricia M.; Creati, Louise; Wood, P.R.; Roberton, Don; Hosking, C. S.

doi:10.1007/bf02072513

Cited by 13 publications

(7 citation statements)

References 9 publications

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“… 4 (4) Furthermore, a NTM‐dominant reaction would establish the diagnosis of the adenitis in only 48 to 72 hours and allow the clinician to comfort the parents as to the benign nature of the lesion. Von Reyn et al 5 have demonstrated, using the same M. avium sensitin as in our institution, that dual skin testing distinguishes between infection resulting from MAC and infection caused by M. tuberculosis : a dominant skin test reaction was 100% specific for infection with the homologous organism. If we only consider the patients with a definitive diagnosis of NTM adenitis, the sensitivity of NTM skin testing alone is 87%.…”

Section: Discussionmentioning

confidence: 80%

“…This might be explained by the 100% specificity demonstrated by von Reyn et al in their study. 5 The role of medical treatment is not well established, 14 and there has been no controlled study to support its usefulness. 15 Medications such as clarithromycin may prove effective, 14 but treatment with antituberculous drugs is usually ineffective because most NTM are resistant to the majority of antituberculous agents.…”

Section: Discussionmentioning

confidence: 99%

“…There have been numerous reports of cervicofacial NTM adenitis in the past two decades. Numerous diagnostic approaches 4–10 and treatment options 1–3,11–16 have been proposed. In this article, we describe our experience with the diagnosis, surgical treatment, and outcome of NTM adenitis of the head and neck in children.…”

Section: Introductionmentioning

confidence: 99%

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Nontuberculous Mycobacterial Adenitis of the Head and Neck in Children: Experience From a Tertiary Care Pediatric Center

et al. 2001

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Nontuberculous Mycobacterial Adenitis of the Head and Neck in Children: Experience From a Tertiary Care Pediatric Center

et al. 2001

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“…Therefore, any condition that alters the immune system, especially that will depress this system, theoretically can cause false-negative results with this test. [12][13][14][15][16] In the US Centers for Disease Control and Protection (CDC) guidelines of 2003, pregnancy was one of the conditions in which use of this test was not recommended, because pregnancy has the potential to decrease the immune response. 7 In the CDC guidelines of 2005, the spectrum to use QuantiFERON ® -TB Gold was broadened and pregnancy no longer showed as one of the conditions for which it is not recommended.…”

Section: Introductionmentioning

confidence: 99%

Use of the QuantiFERON®-TB Gold Assay in Pregnant Patients

Chehab¹,

Kallail²,

Fakih³

et al. 2010

kjm

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Background. The QuantiFERON ® -TB Gold assay is a new test for latent tuberculosis infection. It is thought to be more reliable and have fewer false positives than the tuberculin skin test (TST). Both tests are dependent on a normal immune system for diagnostic accuracy. However, no comparisons of the two tests have assessed the accuracy in pregnant women. This investigation assessed the consistency of results between the two tests in both pregnant and nonpregnant women. Methods. The study included 152 women presenting for care at the Sedgwick County Health Department. They were divided into two groups of pregnant and non-pregnant women. Both groups were assessed with the Quantiferon assay and the TST. Subjects were adults between the ages of 18 and 45. All had a pregnancy test and a negative HIV test. None had existing morbidities that would influence the test results. Results. Concordant results between the tests were shown in 131 subjects (86.2%). Of the pregnant women, 91.2% had concordant results. Of the non-pregnant women, 76% had concordant results. Significantly more discordant results occurred in non-pregnant women (p<.022). Conclusion. Current guidelines favor using either test in healthy individuals. Although more discordant results occurred in the non-pregnant women, both tests were effective in pregnant women. Thus, TST and Quantiferon are accurate to use in pregnant women. The decision to use either test in pregnant women should be based mainly on the compliance of the patient to return to have the TST read. KJM 2010; 3(2):24-30.

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“…Leukocytes from multiple healthy controls showed negligible IFN-␥ responses to both PPD preparations. A comparable ex vivo whole blood assay has been used for diagnosis of paratuberculosis in cattle (58,79), cervical lymphadenitis caused by M. avium in children (17,36), and leprosy (76,77).…”

mentioning

confidence: 99%

Results of Multiple Diagnostic Tests forMycobacterium aviumsubsp.paratuberculosisin Patients with Inflammatory Bowel Disease and in Controls

Collins¹,

Lisby²,

Moser

et al. 2000

J Clin Microbiol

126

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Mycobacterium avium subsp. paratuberculosishas been incriminated as a cause of Crohn's disease (CD); however, studies to date have been relatively small and generally only used a single diagnostic assay. The objective of the study was to reexamine the association of M. avium subsp.paratuberculosis and CD using multiple diagnostic tests. Five methods were used to detect M. avium subsp.paratuberculosis infections in 439 inflammatory bowel disease (IBD) patients and 324 control subjects in the United States and Denmark. Most assays were adaptations of diagnostic tests for this infection performed routinely on animals. PCR for IS900, a genetic element unique to M. avium subsp.paratuberculosis, was positive significantly more often on resected bowel and lymph node tissues from CD patients (19.0%) and ulcerative colitis (UC) patients (26.2%) than from controls (6.3%) (P < 0.05). Positive IS900 PCR results occurred more often in U.S. than in Danish IBD patients, 32.0 versus 13.3% (P = 0.025). The majority of Danish patients were bacillus Calmette-Guérin (Mycobacterium bovisBCG) vaccinated (CD, 77.5%; UC, 86.6%; controls, 83.0%) whereas none of the U.S. patients with IBD and only 2% of U.S. controls were vaccinated. Among Danish IBD patients, positive PCR findings were four times more common among subjects who were not BCG vaccinated (33.3%) than among BCG vaccinates (8.8%, P = 0.02). Culture of the same tissues tested by PCR using modified BACTEC 12B medium failed to grow M. avium subsp. paratuberculosisfrom patients or controls. U.S. CD patients had the highest serological evidence (enzyme-linked immunosorbent assay [ELISA] for serum antibodies) of M. avium subsp. paratuberculosisinfection (20.7% of patients positive) which was higher than for all UC patients studied (6.1%) or healthy controls (3.8%,P < 0.005). Among Danish patients alone, however, no significant differences in rates of ELISA-positive results among CD, UC, or control patients were found. For 181 study subjects, both IS900 PCR and ELISA were performed. Although 11 were ELISA positive and 36 were PCR positive, in no instance was a patient positive by both tests, suggesting that these states are mutually exclusive. Evaluation of cytokine-mediated immune responses of IBD patients was complicated by the influence of immunosuppressive therapy given most IBD patients. Gamma interferon (IFN-γ) release by peripheral blood leukocytes after M. avium purified protein derivative PPD antigen stimulation showed significantly lower responses in CD patients than in UC patients or controls in both U.S. (by ex vivo assay) and Danish (by in vitro assay) populations (P< 0.05). Interleukin-5 responses were not different among CD, UC, or control groups. Collectively, the PCR, ELISA, and IFN-γ tests forM. avium subsp. paratuberculosis together with the unexpected observation that BCG vaccination influenced M. avium subsp. paratuberculosis detection, lead us to conclude that M. avium subsp. paratuberculosis, or some similarly fastidious mycobacterial species, infects at least a subset of IBD patients. Whether the infection is primary (causal) or secondary, it may contribute to the etiopathogenesis of IBD.

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Lymphocyte production of gamma-interferon as a test for non-tuberculous mycobacterial lymphadenitis in childhood

Cited by 13 publications

References 9 publications

Nontuberculous Mycobacterial Adenitis of the Head and Neck in Children: Experience From a Tertiary Care Pediatric Center

Nontuberculous Mycobacterial Adenitis of the Head and Neck in Children: Experience From a Tertiary Care Pediatric Center

Use of the QuantiFERON®-TB Gold Assay in Pregnant Patients

Results of Multiple Diagnostic Tests forMycobacterium aviumsubsp.paratuberculosisin Patients with Inflammatory Bowel Disease and in Controls

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