Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing.

Abstract

Monoclonal antibodies (MAb) have been used to probe the relationship of cytolytic T lymphocyte (CTL) surface molecules to CTL function. Rat MAb to mouse CTL were generated. Twelve MAb so obtained gave preferential binding to T cells as compared to B cells, and three of these recognized previously undescribed surface polypeptides. These Mab and more broadly reactive and previously obtained MAb were tested for their ability to block CTL-mediated killing in the absence of complement. To ensure that any observed blocking was due to binding ofMAb to the effector cell rather than the target cell, a xenogeneic mouse CU anti-rat BN lymphoma target cell system was utilized (MAb and target cells both of rat origin). Of 24 MAb tested here, 21 had little or no effect on CTL function, including those to H-

Differential endocytosis of T and B lymphocyte surface molecules evaluated with antibody-bearing fluorescent liposomes containing methotrexate.

“…A second hypothesis is that the target molecules to which (9,27). The present data indicate that the p94,180 molecule is internalized by Tbut not B cells.…”

Prevention of adoptive transfer of murine Sjögren's syndrome into severe combined immunodeficient (SCID) mice by antibodies against intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1)

“…It was reported that the role of ICAM-I during activation of mouse T cells has shown that CD4 Th cells responded to a lower concentration of antigen presented on MHC class Il-expressing eells, which were cotransfected with ICAM-1 [34]. The LF"A-1 molecule mediates lymphocyte adhesion to vascular endothelial cells, and promotes killer T cell-mediated target eell lysis and T cell proliferation [35][36][37][38]. The density of LFA-l on lymphocytes controls their adhesive properties to endothelial cells [10].…”

Inhibition of human lectin-dependent cell-mediated cytotoxicity, natural killer-like cytotoxicity, and cytotoxic T-lymphocyte-mediated cytolysis by xenoantisera raised against concanavalin A-stimulated human lymphocytes

“…Fresh PBL were less effective in adsorbing the inhibitory antibodies, nevertheless three sequential adsorptions with 4 X 10' fresh PBL/ml of antiserum abrogated the inhibitory capacity of these antisera (data not shown). Recent studies have suggested that effector cells display new molecules, or new determinants on molecules already present, during interaction with target cells (19,27). Therefore we performed the following experiment to determine whether P3 antiserum recognizes molecules or determinants preferentially expressed during target-effector cell interaction.…”

“…Furthermore the determination that an antiserum inhibits a particular phase of cytolysis provides a good indication that a cell-surface molecule recognized by that antiserum is involved in that phase of lysis. A number of investigators have recently reported the production of antiserum and monoclonal antibodies that inhibit both human (15)(16)(17) and murine (14,18,19) lymphocytemediated cytolysis in vitro. However, only two antisera have been shown to inhibit cytolysis after the recognition phase.…”