Lymphocyte function-associated antigen-1 binding residues in intercellular adhesion molecule-2 (ICAM-2) and the integrin binding surface in the ICAM subfamily

Casasnovas, José M.; Pieroni, Cristiana; Springer, Timothy A.

doi:10.1073/pnas.96.6.3017

Cited by 31 publications

(30 citation statements)

References 35 publications

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“…Transfections using the DEAEdextran method were essentially as described (12). Each experiment included transfection with 0.1-8.0 g of wild type and 6 g of mutant DNAs.…”

Section: Methodsmentioning

confidence: 99%

“…Plasmids and Mutagenesis-Mutagenesis of ICAM-1 and ICAM-2 was carried by the overlap PCR technique using PfuI polymerase (Stratagene) and the cDNAs cloned in the unique XbaI site of pAprM9 (12,17). Mutants were subcloned either in the pAprM9 vector for cell surface expression or in the pEF vector (27) for the preparation of soluble proteins.…”

Section: Methodsmentioning

confidence: 99%

“…A cell binding assay with purified and plastic coated LFA-1 was carried out as described in detail elsewhere (12 (16). Virus solution was aspirated, and wells were washed two times with 50 l of phosphate-buffered saline, 5% fetal calf serum.…”

Section: Methodsmentioning

confidence: 99%

“…About 1 ϫ 10 5 cells were incubated with anti-ICAM antibodies at 10 g/ml. The percentage of cells expressing antibody epitopes was determined after subtraction of background staining from cells mock transfected with pAprM9 (12).…”

Section: Methodsmentioning

confidence: 99%

“…Alternatively, the interaction of DC-SIGN with ICAM-3 establishes initial contact between dendritic cells and resting T-cells during antigen presentation (11). Whereas the integrin binding surface in ICAMs is glycan-free (12,13), the recognition of DC-SIGN is dependent on carbohydrates (10). The lectin binds with high affinity to high mannose and fucose-containing carbohydrates (14).…”

mentioning

confidence: 99%

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Contribution of N-Linked Glycans to the Conformation and Function of Intercellular Adhesion Molecules (ICAMs)

Jiménez

Roda-Navarro

Springer³

et al. 2005

Journal of Biological Chemistry

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“…Transfections using the DEAEdextran method were essentially as described (12). Each experiment included transfection with 0.1-8.0 g of wild type and 6 g of mutant DNAs.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Contribution of N-Linked Glycans to the Conformation and Function of Intercellular Adhesion Molecules (ICAMs)

Jiménez

Roda-Navarro

Springer³

et al. 2005

Journal of Biological Chemistry

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Binding of T lymphocytes to hippocampal neurons through ICAM-5 (telencephalin) and characterization of its interaction with the leukocyte integrin CD11a / CD18

et al. 2000

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Intercellular adhesion molecule‐5 (ICAM‐5, telencephalin) is a member of the immunoglobulin superfamily expressed on telencephalic neurons, and serves as a ligand for the leukocyte integrin CD11a/CD18. We studied here the binding site in ICAM‐5 for CD11a/CD18. Protein constructs containing the first immunoglobulin domain of ICAM‐5 were able to support CD11a/CD18 interaction, while deletion of the first domain abolished binding. Monoclonal antibodies reacting with the first domain of ICAM‐5 also completely blocked the interaction. The soluble first domain of ICAM‐5 inhibited the binding of T cells to immobilized ICAM‐5 at concentrations of 50 nM and higher. Interestingly, the sixth domain of ICAM‐5 was also able to support leukocyte binding, but this binding activity may not involve leukocyte integrins. To test the involvement of ICAM‐5 in leukocyte‐neuron interactions, an assay using human T cells binding to rat hippocampal neurons was established. This binding was blocked by monoclonal antibodies against CD11a/CD18 and ICAM‐5. Thus ICAM‐5 may act as a major adhesion molecule for leukocyte binding to neurons in the central nervous system.

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Cellular immune surveillance of central nervous system bypasses blood–brain barrier and blood–cerebrospinal–fluid barrier: Revealed with the New Marburg cerebrospinal–fluid model in healthy humans

Kleine¹

2015

Cytometry Pt A

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In healthy human brain/spinal cord, blood capillaries and venules are locked differently with junctions and basement membrane (blood-brain barrier, blood-venule barrier). In choroid plexus, epithelial tight junctions and basement membrane lock bloodcerebrospinal-fluid (CSF) barrier. Lymphocytic cell data, quantified with multicolour flow-cytometry or immuno-cytochemical methods in sample pairs of lumbar CSF, ventricular CSF and peripheral venous blood, are taken from references; similarly, data of thoracic duct chyle and blood sample pairs. Through three circumventricular organs (median eminence, organum vasculosum lamina terminalis, area postrema), 15-30 ll blood are pressed by blood pressure through fenestrated capillaries, matrix/basement membrane spaces and ependyma cell lacks into ventricular/suboccipital CSF to generate CD31 , CD4 CD31 cell counts in ventricular and lumbar CSF, higher CD31 subsets in lumbar CSF, and few NK and B cells due to absorption in circumventricular organs. The Marburg CSF Model reflects origin and turnover of lymphatic cells in CSF realistically; the model differs from ligand-multistep processes of activated lymphocytes through blood-brain-, blood-venule-, and blood-CSF-barriers; because transfer of inactivated native lymphocytes through the barriers is not found with healthy humans, although described so in literature. V C 2015 International Society for Advancement of Cytometry

show abstract

Lymphocyte function-associated antigen-1 binding residues in intercellular adhesion molecule-2 (ICAM-2) and the integrin binding surface in the ICAM subfamily

Cited by 31 publications

References 35 publications

Contribution of N-Linked Glycans to the Conformation and Function of Intercellular Adhesion Molecules (ICAMs)

Contribution of N-Linked Glycans to the Conformation and Function of Intercellular Adhesion Molecules (ICAMs)

Binding of T lymphocytes to hippocampal neurons through ICAM-5 (telencephalin) and characterization of its interaction with the leukocyte integrin CD11a / CD18

Cellular immune surveillance of central nervous system bypasses blood–brain barrier and blood–cerebrospinal–fluid barrier: Revealed with the New Marburg cerebrospinal–fluid model in healthy humans

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