Lymphocyte DNA damage in Turkish asphalt workers detected by the comet assay

Bacaksız, Ayşegül; Kayaaltı, Zeliha; Tutkun, Engın; Söylemezoğlu, Tülın

doi:10.1080/09603123.2013.773586

Cited by 13 publications

(10 citation statements)

References 38 publications

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“…In this assay, the level of DNA damage in the population studied was <4% (tail). This type of identification of damage to genetic material has previously been used to evaluate exposure to environmental or occupational factors, and for diagnosis of cancer or diseases resulting from impaired DNA repair mechanisms (Gedik et al 1992;Jophnson and Ferris 2005;Bacaksiz et al 2014). It has also found application in assessing the quality of semen in animals (Fraser and Strzeżek 2004).…”

Section: Discussionmentioning

confidence: 99%

Analysis of chromatin instability of somatic cells in sheep

et al. 2018

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Cytogenetic tests are highly reliable, sensitive indicators of the early effects of genomic instability. Their results provide information on the organism’s susceptibility to exogenous and endogenous factors and are measures of the degree of repair of DNA damage. Our study assessed spontaneously occurring damage in following four breeds of sheep: Polish Heath, Polish Lowland (Zelazna variety), Polish Blackhead, and Berrichon du Cher. Instability was identified using the following three different tests: a sister chromatid exchange (SCE) assay, identification of fragile sites, and the comet assay. The distribution of instabilities varied depending on the breed. The mean frequency of SCEs was 5.13 ± 1.58, whereas that of fragile sites was 3.30 ± 1.24. The mean level of DNA damage (% head DNA) was 96.52 ± 6.59. The most damage to genetic material was observed in the Berrichon du Cher sheep, and the least in the Polish Heath sheep. The tests used are reliable biomarkers of genome stability in animal breeds, as well as in individuals within breeds.

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Section: Discussionmentioning

confidence: 99%

Analysis of chromatin instability of somatic cells in sheep

et al. 2018

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“…Due to its wide variety of applications, comet assay is utilized by many researchers to assess DNA damage in the workers of different industries. During the literature review, a number of health biomonitoring studies were found that used comet assay (12)(13)(14)(15)(16). Those studies were aimed at assessing the amount of genotoxicity caused by particular exposures in the workers of different industries, such as open-cast coal mining (17), the automotive industry (18), lead battery recycling plants (19), and the petrol industry (20).…”

Section: Resultsmentioning

confidence: 99%

“…Comet assay could be recognized as an excellent marker and a supplementary technique for monitoring the presence or absence of genotoxic effects. Another study used comet assay to detect DNA damage in the blood lymphocytes of 30 workers exposed to asphalt fumes and 30 non-exposed controls (15). DNA damage was evaluated by the percentage of the DNA present in the comet tail (% tail DNA) in each cell.…”

Section: Resultsmentioning

confidence: 99%

Comet assay as a sensitive technique in occupational health studies; A literature review

Singh

2018

JOHE

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Article InfoBackground: Occupational health is a sensitive field in many countries where occupational exposure needs attention due to the lack of awareness. Several businesses, including leather, textile, iron, steel, and dyeing industries use a variety of mutagenic and genotoxic chemicals. Exposure to these chemicals may pose serious health risks, including genetic damage to workers. Comet assay is a technique that has been extensively used in assessing DNA damage in such workers. Materials and methods: The literature search was done using PubMed and Google Scholar databases. Some studies were found reporting the use of comet assay as a sensitive and useful technique in assessing genetic damage in occupationally exposed workers. These studies have been presented in this paper to emphasize the importance and propriety of comet assay in assessing the genetic impact of hazardous exposures. Result: Having gone through the available reports, the author of this paper found comet assay as a useful biotechnological technique in the field of occupational biomonitoring. This technique is a sensitive and cheap method for assessing genotoxicity. Conclusion: Comet assay can be used as a reliable technique to quantify the impact of genotoxicants in the form of DNA damage at a single-cell level. This assay can be considered as a sensitive parameter whenever health assessment studies are to be designed.

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“…This instability generates further mutations, which may lead to developmental abnormalities, embryo death, reproductive disorders, and cause neoplastic transformation (Ashby, Tinwell, Lefevre, & Browne, 1995;Wójcik, Andraszek, Ciszewska, & Smalec, 2013). The comet assay is a sensitive test used to measure DNA damage and repair in single cells (Ashby et al, 1995 (Bacaksiz, Kayaalti, Soylemez, Tutkun, & Solemezoglu, 2014;Gedik, Ewen, & Collins, 1992). In animals, it was used to evaluate semen quality in the boar (Fraser, Parda, Filipowicz, & Strzeżek, 2010) and chinchilla (Niedbała et al, 2015), and to assess chromatin instability in the somatic cells of sheep (Wójcik et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Determination of cytogenetic markers for biological monitoring in coypu (Myocastor coypu)

Kuchta-Gładysz

Wójcik

Słonina

et al. 2020

Animal Science Journal

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Coypus (Myocastor coypu) are fur-bearing herbivores native to South America (Gosling & Berker, 1991). In European countries, coypus have been bred since the 1920s (Carter & Leonard, 2002). In Poland, coypu breeding experienced a decline in the late 1990s and the number of animals began to fall quickly. Although coypu pelts and meat are valuable, there was no demand for these products (Kowalska & Niedbała, 2014). Furthermore, coypu breeding was adversely affected by environmental movement campaigns and rising farm maintenance costs. For this reason, coypus were included in a genetic resources conservation program for native breeds, which is aimed to preserve and increase population size while maintaining genetic variation in the coypu (http://www.bioro znoro dnosc.izoo.krakow. pl). The loss of interest in the breeding of this species did not prevent researchers from conducting numerous studies, including the determination of genetic diversity in the populations and karyotype characterization using available cytogenetic techniques (Kaplanova et al., 2012). In the coypu karyotype (2n = 42), all the autosomes are bi-armed: 32 are metacentric and 8 are submetacentric. The X sex chromosome is metacentric and the Y is the smallest chromosome of

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Lymphocyte DNA damage in Turkish asphalt workers detected by the comet assay

Cited by 13 publications

References 38 publications

Analysis of chromatin instability of somatic cells in sheep

Analysis of chromatin instability of somatic cells in sheep

Comet assay as a sensitive technique in occupational health studies; A literature review

Determination of cytogenetic markers for biological monitoring in coypu (Myocastor coypu)

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