Lymphocyte calcium influx kinetics in multiple sclerosis treated without or with interferon beta

Experimental Conditions and Mathematical Analysis of Kinetic Measurements Using Flow Cytometry – The FacsKin Method

“…In healthy individuals, a triarylmethane compound (TRAM), the specific inhibitor of the IKCa1 channel decreased calcium influx in Th2 cells to a lower extent than in Th1 cells (Toldi et al, 2011b). This finding supports previous data from Fanger et al (2000) obtained by patch clamp, showing that IKCa1 currents are smaller in Th2 cells when compared with the Th1 subset.…”

“…We also investigated calcium influx kinetics in multiple sclerosis (MS) patients without and with interferon (IFN) beta treatment compared to healthy individuals (Toldi et al, 2011b). We aimed to describe the effects of Kv1.3 and IKCa1 channel inhibitors on calcium influx, and to assess whether these inhibitors could potentially contribute to the treatment of MS via the selective reduction of lymphocyte activation as suggested before (Wulff et al, 2003).…”

Altered calcium influx of peripheral Th2 cells in pediatric Crohn's disease: infliximab may normalize activation patterns

“…Interestingly, our current results proved to be fairly selective in modulation of the abnormal calcium influx of peripheral Th2 cells, while our pervious studies show only moderate selectivity on CD8 lymphocytes in multiple sclerosis or on Th1 cells in type 1 diabetes mellitus [13, 14]. …”

“…In addition, we also reported that autoimmune disorders have a major impact on T cell calcium homeostasis. During the short-term activation of peripheral T cells in patients with rheumatoid arthritis (RA) [12], multiple sclerosis [13] and type 1 diabetes [14], we found altered intracellular calcium kinetics, that were, at least partly, attributed to altered function and/or expression of lymphocyte potassium channels. We also demonstrated that some immune modulatory therapies, also used in CD, may have an impact on T cell function.…”

Analysis by flow cytometry of calcium influx kinetics in peripheral lymphocytes of patients with rheumatoid arthritis

“…1B), a specific software developed at our laboratory (available at www.facskin.com along with a detailed description of the evaluation process) as well as the kinetic module of FlowJo (v 7.6.3, Fig. 1C (7,11,12). Since AUC is regarded as the best single parameter describing the magnitude of Ca 21 influx, this parameter was also calculated using the kinetic module of FlowJo (v 7.6.3).…”