Lymphocyte Calcium Influx Characteristics and their Modulation by Kv1.3 and IKCa1 Channel Inhibitors in Healthy Pregnancy and Preeclampsia

Abstract

There is a characteristic pattern of calcium influx in T lymphocytes and its sensitivity to potassium channel inhibition in HP that is missing in PE, raising the notion that T-lymphocyte calcium handling may have a role in the characteristic immune status of HP.

Experimental Conditions and Mathematical Analysis of Kinetic Measurements Using Flow Cytometry – The FacsKin Method

“…Th1, Th2, CD4 and CD8 cells) between non-pregnant, healthy pregnant and preeclamptic lymphocytes (Toldi et al, 2010a). These properties in preeclampsia are more comparable to the non-pregnant state than to healthy pregnancy, suggesting that there is a characteristic pattern of calcium influx in healthy pregnant women that is missing in preeclamptic patients.…”

“…Note that these calculated parameters differ from the parameters that would be recorded during the measurement for each cell: from a single measurement one can only derive a single value for each such calculated parameter. Jakubczak et al;2006, Demkow et al;2009, Toldi et al;2010b, Szalay et al, 2012), t-test (used in Lund-Johansen et al, 1992, paired Wilcoxon-test (used in Schepers et al, 2009;Toldi et al, 2010a, Szalay et al, 2012.…”

“…We will describe a version improved further in Section 3. Some applications of this method are described in Section 5 (Toldi et al, 2010a(Toldi et al, -b-c, 2011a. Mechanistic models for calcium flux kinetics are also available (Tang et al, 1996;Politi et al, 2006).…”

T Lymphocyte Characteristics and Immune Tolerance During Human Pregnancy

“…However, we could not detect a correlation between the prevalence of Th17 and Th1 cells (using the cell surface marker, CXCR3 for the identification of the latter subset). Thus, the effect of Th17 cells and IL-17 on the inflammatory status is more likely to be exerted in a direct manner rather than through the modulation of the Th1/Th2 balance in preeclampsia (Toldi et al, 2011a). The alterations of Th17 cells have been observed in other pregnancy-related disorders as well, suggesting that the balance of Th17 cells and Tregs and not only that of Th1 and Th2 cells has crucial effects on the inflammatory status in human pregnancy.…”

“…Indeed, galectin-1-deficient mice were shown to develop greater Th1 and Th17 responses (Toscano et al, 2007). Therefore, it is tempting to speculate that decreased production of galectin-1 by circulating T and NK cells might contribute to the development of the pro-inflammatory Th1 and Th17 immune responses, which are characteristic features of preeclampsia (Saito et al, 1999b;Toldi et al, 2011a).…”