Lymphocyte antigen Leu-19 as a molecular marker of regeneration in human skeletal muscle.

Abstract

Antigen Leu-19 (Leul9-Ag), a 200-to 220-kDa surface glycoprotein, was originally identified on a subset of human peripheral lymphocytes exhibiting non-major histocompatibility complex-restricted cytotoxicity. Here we report that monoclonal antibody Leu-19 (mAb-Leu19) labels structures in human skeletal muscle: (i) satellite cells, which form the stem cell pool of muscle fiber regeneration, both in normal and diseased muscle; (ii) myotubes and myotube projections in regions of muscle fiber repair; (iii) periodically organized fibrillar structures in areas of regeneration; (iv) the surface of myoblasts and developing myotubes in culture. mAb-Leul19 precipitated a protein of -200 kDa from cultured muscle cells. Our data show that Leul9-Ag is expressed on muscle-specific components of myosegments in repair and thus represents a molecular marker of muscle regeneration. On the basis of this molecular marker and using laser scan microscopy, it is possible to visualize at the light microscopic level hitherto undetectable details of muscle regeneration in routine cryostat sections.

Characterization of proliferating human skeletal muscle‐derived cells in vitro: Differential modulation of myoblast markers by TGF‐β2

“…Unlike desmin and creatine kinase, it is absent from mature myocytes (Schubert et al, 1989). Our results showed that CD56 and desmin are differentially regulated by TGF-b2.…”

“…Its up-regulation is a signal of myoblast differentiation. CD56 (also named NCAM or Antigen Leu-19) is expressed constituitively in proliferating myoblasts (Illa et al, 1992;Belles-Isles et al, 1993), but is absent in mature muscle (Schubert et al, 1989). Other cells also express CD56, including certain lymphocytes and neurons, but not fibroblasts.…”

A three‐symbol code for organized proteomes based on cyclical imaging of protein locations

“…Five-micrometer thick cryosections were prepared according to earlier protocols (24): fixed in ice-cold acetone at 220°C for 20 min, air-dried, and then rehydrated in PBS (pH 7.4) at room temperature. Peripheral blood mononuclear cells (PBMC) were prepared as described (25).…”

Expression pattern of M‐cadherin in normal, denervated, and regenerating mouse muscles

“…Among those are cell adhesion molecules (Rutishauser and Jessell, 1988;Edelman, 1992), in particular the neural cell adhesion molecule (NCAM, Rutishauser and Jessell, 1988). NCAM has previously been found on activated satellite cells (myoblasts) and on myotubes during regeneration or on the entire sarcolemma of muscle fibers after denervation (Covault and Sanes, 1985;Moore and Walsh, 1986;Schubert et al, 1989). Thus, NCAM is one molecular marker of regenerating and denervated muscle fibers.…”

“…Thus, NCAM is one molecular marker of regenerating and denervated muscle fibers. In normal adult muscle, NCAM is expressed at the neuromuscular junction (Covault and Sanes, 1986) and has been occasionally observed in satellite cells (Covault and Sanes, 1986;Cashman et al, 1987;Schubert et al, 1989).…”