2010
DOI: 10.1186/1471-2334-10-205
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Lymphocyte and monocyte flow cytometry immunophenotyping as a diagnostic tool in uncharacteristic inflammatory disorders

Abstract: BackgroundPatients with uncharacteristic inflammatory symptoms such as long-standing fatigue or pain, or a prolonged fever, constitute a diagnostic and therapeutic challenge. The aim of the present study was to determine if an extended immunophenotyping of lymphocytes and monocytes including activation markers can define disease-specific patterns, and thus provide valuable diagnostic information for these patients.MethodsWhole blood from patients with gram-negative bacteraemia, neuroborreliosis, tuberculosis, … Show more

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Cited by 25 publications
(28 citation statements)
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“…Flow cytometry is commonly used to quantify and characterize lymphocytes and cytokines in several diseases including cancer (12, 34, 35) and can rapidly analyze up to 18 parameters. However, in solid tumors, it has not seen broad adoption in the clinical setting.…”
Section: Discussionmentioning
confidence: 99%
“…Flow cytometry is commonly used to quantify and characterize lymphocytes and cytokines in several diseases including cancer (12, 34, 35) and can rapidly analyze up to 18 parameters. However, in solid tumors, it has not seen broad adoption in the clinical setting.…”
Section: Discussionmentioning
confidence: 99%
“…Sepsis patients also have reduced TNF-a serum levels, increased p50 homodimer formation, increased pinocytosis, but decreased cytotoxic-and Ag presenting capacity (5,17). In contrast to M2 macrophages, however, but similar to human CD14 + Mo suppressor subpopulations present in cancer patients, tolerant macrophages from sepsis patients show a reduced expression level of HLA-DR and coreceptor downregulation and importantly also show upregulation of TLR4 inhibitory molecules (5,18,19).…”
mentioning
confidence: 90%
“…First, CD16 is also expressed by natural killer (NK) lymphocytes [61]. These cells may be picked up by the gating strategy in which the scatter gate for monocytes extends into the lymphocytes.…”
Section: Discussionmentioning
confidence: 99%