Lymphoblastic lymphoma/leukemia of T-cell origin: Ultrastructural, cytochemical, and immunologic features of ten cases

Palacios, José J. Navas; Valdés, M.D.; Pallares, Maria A. Montalban; Salazar, Maria D. Gomez De; Marcilla, Ana Garcia

doi:10.1002/1097-0142(19811101)48:9<1982::aid-cncr2820480913>3.0.co;2-4

Cited by 24 publications

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

On the histogenesis of Ewing's sarcoma: An ultrastructural, immunohistochemical, and cytochemical study

Navas‐Palacios¹,

Aparicio-Duque²,

Valdés³

1984

Cancer

View full text Add to dashboard Cite

Forty‐two cases of Ewing's sarcoma (ES) have been studied with light microscopy during the 9‐year period 1974 to 1982. Thirty‐three patients had ES of bone, and in 9 patients the tumor was located in the extraskeletal soft tissues. Cases which fulfilled all the morphologic criteria were accepted as typical ES (31 cases), and those with some architectural or cytologic peculiarities were considered atypical forms of ES (11 cases). An immunohistochemical study (PAP method) to evaluate the presence in the tumor cells of the following markers: myoglobin, F‐VIII‐related antigen, lysozyme, alpha‐1‐antitrypsin, alpha‐1‐antichymotrypsin, and immunoglobulins (IgG, IgA, IgM, kappa and lambda light chains), was performed with negative results in all cases (paraffin blocks were available in 38 cases). The cytochemical study on fresh tissue imprints from five patients (PAS, Sudan Black, alpha‐naphthyl acetate esterase, acid phosphatase, beta glucuronidase, myeloperoxidases, naphthol‐AS‐D chloroacetate esterase and alkaline phosphatase) gave no pattern of histogenetic significance, PAS being the best morphologic marker in tissue sections and touch preparations. A detailed ultrastructural study was performed on 34 cases; the main findings may be summarized as follows: medium sized cells, polygonal shape, oval nuclei, smooth nuclear envelope, abundant euchromatin, well‐developed nucleolonema, scant membranous organelles, abundant hyaloplasmic glycogen, occasional lipid vacuoles, straight cell membranes, and primitive intercellular junctions. No differences were found between bone and extraskeletal ES; moreover, typical and atypical forms showed moderate quantitative differences with no qualitative change. The histogenesis is discussed; no functional or morphologic markers have been found to suggest the cell of origin; however, some cell lines may be excluded. It is the impression of the authors that they are dealing with a primitive noncommitted mesenchymal cell.

show abstract

On the histogenesis of Ewing's sarcoma: An ultrastructural, immunohistochemical, and cytochemical study

Navas‐Palacios¹,

Aparicio-Duque²,

Valdés³

1984

Cancer

View full text Add to dashboard Cite

show abstract

Phenotypic and ultrastructural characterization of a medullary thymocyte acute lymphoblastic leukemia with cellular procoagulant activity

Chorba

Orenstein

Ney

et al. 1985

Cancer

View full text Add to dashboard Cite

The phenotypic and ultrastructural characterization of the blast cells from a T‐cell acute lymphoblastic leukemia (T‐ALL) that was associated with disseminated intravascular coagulation (DIC) is described. The bone marrow blasts were considered to represent neoplastic medullary thymocytes and were acid phosphatase (+), terminal deoxynucleotidyl transferase (‐), acid alpha‐naphthyl acetate esterase (‐), E‐rosette (+) at 4°C and 37°C, Fc‐ and C3‐receptor (‐), and cALLA‐, la‐, 9.6+, OKT3+, OKT4+, OKT6+/‐, OKT8+, OKT10+. On transmission electron miscroscopic study, the predominant cell was 6 μm in diameter and possessed an irregular nucleus, moderate‐sized nucleolus, marginated heterochromatin, abundant Golgi elements and granules, and prominent intermediate filaments. The cells were analyzed with normal and factor‐deficient human plasmas and contained significant amounts of tissue factor‐like procoagulant activity. This is the first report of a well‐characterized medullary thymocyte T‐ALL in which DIC was an accompanying feature, and the first demonstration of tissue factor‐like procoagulant activity in acute lymphoblastic leukemia. In view of thrombohemorrhagic phenomena observed in association with other medullary thymocyte leukemias, these findings suggest that tissue factor‐like procoagulant activity may be a characteristic of medullary thymocyte‐derived lymphoid leukemias.

show abstract

Rhabdomyosarcoma presenting with diffuse bone marrow involvement, hypercalcemia and renal failure

Serna

Martı́nez

Valdés

et al. 1988

Med. Pediatr. Oncol.

Self Cite

View full text Add to dashboard Cite

Rhabdomyosarcoma and other small round-cell neoplasms of infancy and childhood frequently involve the bone marrow. An unusual clinical presentation of diffuse bone marrow involvement as the sole manifestation of the disease represents a difficult diagnostic challenge. We report the case of an 18-year-old boy presenting with lower back pain, sternum tenderness, anemia, thrombocytopenia, leukoerythroblastic blood film, hypercalcemia, and renal failure. No solid tumor was found. A diagnosis of rhabdomyoblastic bone marrow metastatic involvement was initially suggested by the morphological picture, and later confirmed by the ultrastructural findings. Hypercalcemia and renal failure were controlled, initially, with furosemide and calcitonin. The patient was treated with intensive chemotherapy, including vincristine, actinomycin D, cyclophosphamide, and doxorubicin. A remission was achieved, lasting 5 months until relapse.

show abstract

Lymphoblastic lymphoma/leukemia of T-cell origin: Ultrastructural, cytochemical, and immunologic features of ten cases

Cited by 24 publications

References 46 publications

On the histogenesis of Ewing's sarcoma: An ultrastructural, immunohistochemical, and cytochemical study

On the histogenesis of Ewing's sarcoma: An ultrastructural, immunohistochemical, and cytochemical study

Phenotypic and ultrastructural characterization of a medullary thymocyte acute lymphoblastic leukemia with cellular procoagulant activity

Rhabdomyosarcoma presenting with diffuse bone marrow involvement, hypercalcemia and renal failure

Contact Info

Product

Resources

About