Lymphatic Tissue Engineering and Regeneration

Alderfer, Laura; Wei, Alicia Y.; Hanjaya‐Putra, Donny

doi:10.1186/s13036-018-0122-7

Cited by 58 publications

(52 citation statements)

References 268 publications

(443 reference statements)

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“…Nonetheless, the importance of a sufficient lymphatic network for optimal tissue metabolism and an intact immune response must not be underestimated. The main function of the lymphatic vasculature is interstitial fluid drainage [5], which is important for fluid homeostasis, immune cell surveillance and trafficking, and lipid absorption [5][6][7][8][9][10]. In inflammatory settings, lymphangiogenesis facilitates the resolution of tissue edema and promotes macrophage and dendritic cell mobilization [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…In inflammatory settings, lymphangiogenesis facilitates the resolution of tissue edema and promotes macrophage and dendritic cell mobilization [11][12][13][14]. If the local immune response is delayed, or interstitial edema reduces cellular gas exchange and nutrition (by increasing diffusion distance), the wound healing process is ultimately impaired [6,15,16].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Hypoxia Preconditioned Secretomes on Lymphangiogenic and Angiogenic Sprouting: An in Vitro Analysis

et al. 2020

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Hypoxia Preconditioned Plasma (HPP) and Serum (HPS) are two blood-derived autologous growth factor compositions that are being clinically employed as tools for promoting tissue regeneration, and have been extensively examined for their angiogenic activity. As yet, their ability to stimulate/support lymphangiogenesis remains unknown, although this is an important but often-neglected process in wound healing and tissue repair. Here we set out to characterize the potential of hypoxia preconditioned secretomes as promoters of angiogenic and lymphangiogenic sprouting in vitro. We first analysed HPP/HPS in terms of pro- (VEGF-C) and anti- (TSP-1, PF-4) angiogenic/lymphangiogenic growth factor concentration, before testing their ability to stimulate microvessel sprouting in the mouse aortic ring assay and lymphatic sprouting in the thoracic duct ring assay. The origin of lymphatic structures was validated with lymph-specific immunohistochemical staining (Anti-LYVE-1) and lymphatic vessel-associated protein (polydom) quantification in culture supernatants. HPP/HPS induced greater angiogenic and lymphatic sprouting compared to non-hypoxia preconditioned samples (normal plasma/serum), a response that was compatible with their higher VEGF-C concentration. These findings demonstrate that hypoxia preconditioned blood-derived secretomes have the ability to not only support sprouting angiogenesis, but also lymphangiogenesis, which underlines their multimodal regenerative potential.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Hypoxia Preconditioned Secretomes on Lymphangiogenic and Angiogenic Sprouting: An in Vitro Analysis

et al. 2020

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“…The lymphatic system works synergistically with the blood microvasculature in maintaining the various organs in the body and is one of the vasculature components that should be included in tissueengineered models in the future. 105 Although the lymphatic vasculature has not been the primary focus of this review, many of the models and techniques shown here for blood microvasculature formation may also be used for lymphatic vasculature. However, additional modifications, such as changing the type of matrices, may need to be made when incorporating lymphatic ECs in tissue-engineered models.…”

Section: Discussionmentioning

confidence: 99%

Engineering tissue‐specific blood vessels

Herron

Hansen

Abaci

2019

Bioengineering & Transla Med

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Vascular diversity among organs has recently become widely recognized. Several studies using mouse and human fetal tissues revealed distinct characteristics of organ‐specific vasculature in molecular and functional levels. Thorough understanding of vascular heterogeneities in human adult tissues is significant for developing novel strategies for targeted drug delivery and tissue regeneration. Recent advancements in microfabrication techniques, biomaterials, and differentiation protocols allowed for incorporation of microvasculature into engineered organs. Such vascularized organ models represent physiologically relevant platforms that may offer innovative tools for dissecting the effects of the organ microenvironment on vascular development and expand our present knowledge on organ‐specific human vasculature. In this article, we provide an overview of the current structural and molecular evidence on microvascular diversity, bioengineering methods used to recapitulate the microenvironmental cues, and recent vascularized three‐dimensional organ models from the perspective of tissue‐specific vasculature.

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“…This model could additionally be interrogated to better understand how bone marrow mesenchymal stem cells contribute to metastasis by angiogenesis. 129 Techniques, including cell sheet engineering and coculture systems, which have been used to engineer vasculature can similarly be applied to the lymphatic field, in the way that Marino et al successfully engineered prevascularized dermoepidermal human skin grafts containing lymphatic capillaries. 130 Alimperti et al developed a vessel-on-a-chip with perfused endothelialized channels to mimic the barrier function of the vasculature and recapitulate the endothelial barrier between circulating blood and tissue.…”

Section: Engineering Vasculature and Lymphaticsmentioning

confidence: 99%

Tissue Engineering Models for the Study of Breast Neoplastic Disease and the Tumor Microenvironment

Premaratne

Toyoda

Celie

et al. 2020

Tissue Engineering Part B: Reviews

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The development of adequate experimental models is crucial to furthering the current mechanistic understanding of the etiopathogenesis, subsequent growth, and ultimate metastasis of breast cancer, to develop targeted diagnostics and therapeutics such as the identification of new treatments for multidrug-resistant tumors and triple-negative breast cancers. The utility of new therapeutic options is limited by the platforms currently used to test their efficacy in vitro. The use of three-dimensional models, which incorporate patient-specific, primary cells, offers significant advantages over traditional two-dimensional models by providing a means of accurately recapitulating the complex tumor microenvironment. Advances in breast cancer models, in turn, stand to contribute to more efficacious breast cancer therapeutics. Herein, we review the recent advances in experimental models of breast cancer and suggest methods by which these can be used to further our understanding of said cancer.

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Lymphatic Tissue Engineering and Regeneration

Cited by 58 publications

References 268 publications

Effect of Hypoxia Preconditioned Secretomes on Lymphangiogenic and Angiogenic Sprouting: An in Vitro Analysis

Effect of Hypoxia Preconditioned Secretomes on Lymphangiogenic and Angiogenic Sprouting: An in Vitro Analysis

Engineering tissue‐specific blood vessels

Tissue Engineering Models for the Study of Breast Neoplastic Disease and the Tumor Microenvironment

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