Lymphatic Pump Treatment Mobilizes Leukocytes from the Gut Associated Lymphoid Tissue into Lymph

Hodge, Lisa; Bearden, Melissa K.; Schander, Artur; Huff, Jamie B.; Williams, Arthur G.; King, Hollis H.; Downey, H. Fred

doi:10.1089/lrb.2009.0011

Cited by 42 publications

(54 citation statements)

References 22 publications

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“…Therefore, the objective of this study was to develop a rat model to determine if experimental LPT would increase lymph flow and leukocyte concentration as observed in dogs. 14,15 Our results show that LPT increases the flux of lymphocytes in the cisterna chyli (CC) of rats, thus demonstrating that this treatment produces similar effects in two distinct mammalian models. Furthermore, we have developed a small animal model to investigate the effects of LPT on the lymphatic and immune systems.…”

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confidence: 69%

“…15 Therefore, to determine if LPT mobilizes GALT derived lymphocytes into the CC of rats, we stained lymphocytes collected from the CC for the gut homing leukointegrin a4b7. 16 Approximately 40% of the lymphocytes in the CC stained positive for.…”

Section: Lpt Mobilizes Gut-associated Lymphocytes Into CC Lymphmentioning

confidence: 99%

“…12 In dogs, LPT has been shown to enhance lymph flow and leukocyte numbers in the thoracic 13,14 and mesenteric lymph ducts. 15 Furthermore, LPT was shown to mobilize lymphocytes from the mesenteric lymph nodes into thoracic duct lymph. 15 Collectively, these reports suggest that LPT can enhance function of the lymphatic and immune systems, which may facilitate the clearance of infection; however, the mechanism(s) by which LPT facilitate(s) the clearance of bacteria during infection has not been identified.…”

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confidence: 99%

“…While the dog has been used to study the effect of LPT on the lymphatic system, [13][14][15] there are several limitations to the use of dogs for the study LPT on infectious and inflammatory diseases. These limitations include animal cost and the availability of reagents for immunological studies.…”

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confidence: 99%

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Lymphatic Pump Treatment Augments Lymphatic Flux of Lymphocytes in Rats

Huff

Schander

Downey

et al. 2010

Lymphatic Research and Biology

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Background: Lymphatic pump techniques (LPT) are used by osteopathic practitioners for the treatment of edema and infection; however, the mechanisms by which LPT enhances the lymphatic and immune systems are poorly understood. Methods and Results: To measure the effect of LPT on the rat, the cisterna chyli (CC) of 10 rats were cannulated and lymph was collected during 4 min of 1) pre-LPT baseline, 2) 4 min LPT, and 3) 10 min post-LPT recovery. LPT increased significantly (p < 0.05) lymph flow from a baseline of 24 AE 5 ml/min to 89 AE 30 ml/min. The baseline CC lymphocyte flux was 0.65 AE 0.21Â10 6 lymphocytes/min, and LPT increased CC lymphocyte flux to 6.10 AE 0.99Â10 6 lymphocytes/min (p < 0.01). LPT had no preferential effect on any lymphocyte population, since total lymphocytes, CD4+ T cells, CD8+ T cells, and B cell numbers were similarly increased. To determine if LPT mobilized gut-associated lymphocytes into the CC lymph, gut-associated lymphocytes in the CC lymph were identified by staining CC lymphocytes for the gut homing receptor integrin a4b7. LPT significantly increased (p < 0.01) the flux of a4b7 positive CC lymphocytes from a baseline of 0.70 AE 0.03Â10 5 lymphocytes/min to 6.50 AE 0.10Â10 5 lymphocytes/min during LPT. Finally, lymphocyte flux during recovery was similar to baseline, indicating the effects of LPT are transient. Conclusions: Collectively, these results suggest that LPT may enhance immune surveillance by increasing the numbers of lymphocytes released in to lymphatic circulation, especially from the gut associated lymphoid tissue. The rat provides a useful model to further investigate the effect of LPT on the lymphatic and immune systems.

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confidence: 69%

Section: Lpt Mobilizes Gut-associated Lymphocytes Into CC Lymphmentioning

confidence: 99%

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Lymphatic Pump Treatment Augments Lymphatic Flux of Lymphocytes in Rats

Huff

Schander

Downey

et al. 2010

Lymphatic Research and Biology

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“…Lymphatic pump techniques have been shown to increase cytokines and chemokines in thoracic and intestinal lymph vessels 35 , while mechanical pressure to certain body regions distant from the location of lymph formation enhances flow into lymphatic systems 36 . In addition, it has been shown that lymphatic OMT augments leukocyte mobilization and flow in dogs and rats, primarily from gut-associated lymphoid tissue 37,38,39,40,41 .…”

Section: Representative Resultsmentioning

confidence: 99%

Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia

Yao

Hassani

Gagné

et al. 2014

JoVE

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Pneumonia, the inflammatory state of lung tissue primarily due to microbial infection, claimed 52,306 lives in the United States in 2007 1 and resulted in the hospitalization of 1.1 million patients 2 . With an average length of in-patient hospital stay of five days 2 , pneumonia and influenza comprise significant financial burden costing the United States $40.2 billion in 2005 3 . Under the current Infectious Disease Society of America/American Thoracic Society guidelines, standard-of-care recommendations include the rapid administration of an appropriate antibiotic regiment, fluid replacement, and ventilation (if necessary). Non-standard therapies include the use of corticosteroids and statins; however, these therapies lack conclusive supporting evidence 4 . (Figure 1) Osteopathic Manipulative Treatment (OMT) is a cost-effective adjunctive treatment of pneumonia that has been shown to reduce patients’ length of hospital stay, duration of intravenous antibiotics, and incidence of respiratory failure or death when compared to subjects who received conventional care alone 5 . The use of manual manipulation techniques for pneumonia was first recorded as early as the Spanish influenza pandemic of 1918, when patients treated with standard medical care had an estimated mortality rate of 33%, compared to a 10% mortality rate in patients treated by osteopathic physicians 6 . When applied to the management of pneumonia, manual manipulation techniques bolster lymphatic flow, respiratory function, and immunological defense by targeting anatomical structures involved in the these systems 7,8, 9, 10 . The objective of this review video-article is three-fold: a) summarize the findings of randomized controlled studies on the efficacy of OMT in adult patients with diagnosed pneumonia, b) demonstrate established protocols utilized by osteopathic physicians treating pneumonia, c) elucidate the physiological mechanisms behind manual manipulation of the respiratory and lymphatic systems. Specifically, we will discuss and demonstrate four routine techniques that address autonomics, lymph drainage, and rib cage mobility: 1) Rib Raising, 2) Thoracic Pump, 3) Doming of the Thoracic Diaphragm, and 4) Muscle Energy for Rib 1. 5,11

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Evidence-based assessment of potential therapeutic effects of adjunct osteopathic medicine for multidisciplinary care of acute and convalescent COVID-19 patients

Marin¹,

Maxel²,

Robin³

et al. 2021

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Highlights OMT modulates inflammation and immunity, reduces mortality & hospital length of stay OMT can decrease symptoms of acute patients and boost conventional care efficiency OMT can improve the convalescence of patients and reduce long-term consequences OMT has a substantial part to play in multidisciplinary care against pandemics

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Lymphatic Pump Treatment Mobilizes Leukocytes from the Gut Associated Lymphoid Tissue into Lymph

Abstract: LPT mobilizes leukocytes from GALT, and these leukocytes are transported by the lymphatic circulation. This enhanced release of leukocytes from GALT may provide scientific rationale for the clinical use of LPT to improve immune function.

Cited by 42 publications

References 22 publications

Lymphatic Pump Treatment Augments Lymphatic Flux of Lymphocytes in Rats

Lymphatic Pump Treatment Augments Lymphatic Flux of Lymphocytes in Rats

Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia

Evidence-based assessment of potential therapeutic effects of adjunct osteopathic medicine for multidisciplinary care of acute and convalescent COVID-19 patients

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