Lymphatic growth factors are expressed in human gingiva and upregulated in gingival fibroblasts after stimulation

Bletsa, Athanasia; Abdalla, Hisham; Løes, Sigbjørn; Berggreen, Ellen

doi:10.1002/jper.17-0400

Cited by 6 publications

(10 citation statements)

References 51 publications

(61 reference statements)

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“…The HGFs were isolated and cultured as previously described. 24 All HGFs were cultured in α-minimum essential medium (α-MEM; Hyclone, Logan, UT, USA) containing 10% FBS (Gibco, Grand Island, NY, USA) at 37°C with 5% CO 2 in a cell incubator.…”

Section: Cell Culturementioning

confidence: 99%

<p>Eldecalcitol Inhibits LPS-Induced NLRP3 Inflammasome-Dependent Pyroptosis in Human Gingival Fibroblasts by Activating the Nrf2/HO-1 Signaling Pathway</p>

Huang¹,

Zhang²,

Yang³

et al. 2020

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Section: Cell Culturementioning

confidence: 99%

<p>Eldecalcitol Inhibits LPS-Induced NLRP3 Inflammasome-Dependent Pyroptosis in Human Gingival Fibroblasts by Activating the Nrf2/HO-1 Signaling Pathway</p>

Huang¹,

Zhang²,

Yang³

et al. 2020

DDDT

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“…In a study on sheep injected with radioactive albumin in gingiva, as much as 64.7% of the injected protein was recovered in the prescapular and cervical lymph vessels 7.5 h after injection (15). The gingiva is well supplied with lymphatic vessels (7,16,17), and when lymph is formed, muscle movements and soft tissue deformation may promote a rapid transport of fluid via the lymphatic system. This is in contrast to the bone‐embedded interstitial tissue in the mandibular canal, and may explain why we observed a much slower washout rate from this compartment.…”

Section: Discussionmentioning

confidence: 99%

“…The specificity of all immune reactions was tested by omission of the primary antibodies or substitution with isotype controls. Gingiva from the sectioned jaws known for the presence of lymphatics (7) served as positive control.…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal

Virtej

Tenstad

Wiig

et al. 2020

European J Oral Sciences

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Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal Virtej A, Tenstad O, Wiig H, Berggreen E. Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal. Eur J Oral Sci 2020; 128: 190-195.We sought to investigate the transport route for protein-rich fluid from the apical area towards the draining lymph nodes. The first mandibular molar root canals in 24 female Wistar rats were instrumented and filled with radioactive-labelled human serum albumin. The rats were sacrificed at different intervals beginning after 10 min (time 0) and continuing up to 72 h. Three jaw segments, gingiva around the first molar, blood samples, submandibular and cervical lymph nodes were collected and analyzed for radioactivity. The starting volume of tracer (control) for all experiments was calculated from measurements at time 0. At time 0, radioactivity was only detected in the jaw segments. Within lymph nodes and serum, the tracer was found after 4 h, with the highest amount recorded in serum up to 24 h. Lymphatics were found within the mandibular canal along blood vessels and nerves and exiting via foramen mandibularis, after immunohistochemical staining in four untreated rats. Our results show tracer distribution from the apical area towards the mandibular canal in a posterior direction. The tracer washout rate was low, and the fluid was mainly absorbed into blood vessels. The lymphatics in the mandibular canal may be more important for immune cell transport than for fluid drainage.

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“…After challenging with lipopolysaccharide (LPS) or IL-6/sIL-6R complex, gingival fibroblasts increased the secretion and gene expression of VEGF-A and -C compared to non-induced controls, while VEGF-D was not detected. Altogether, VEGF-A and -C contributes to both the angiogenesis and lymphangiogenesis processes in the pathogenesis of periodontitis [109].…”

Section: Periodontitis and Peri-implantitismentioning

confidence: 99%

Vascular Endothelial Growth Factor: A Translational View in Oral Non-Communicable Diseases

et al. 2021

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Vascular endothelial growth factors (VEGFs) are vital regulators of angiogenesis that are expressed in response to soluble mediators, such as cytokines and growth factors. Their physiologic functions include blood vessel formation, regulation of vascular permeability, stem cell and monocyte/macrophage recruitment and maintenance of bone homeostasis and repair. In addition, angiogenesis plays a pivotal role in chronic pathologic conditions, such as tumorigenesis, inflammatory immune diseases and bone loss. According to their prevalence, morbidity and mortality, inflammatory diseases affecting periodontal tissues and oral cancer are relevant non-communicable diseases. Whereas oral squamous cell carcinoma (OSCC) is considered one of the most common cancers worldwide, destructive inflammatory periodontal diseases, on the other hand, are amongst the most prevalent chronic inflammatory conditions affecting humans and also represent the main cause of tooth loss in adults. In the recent years, while knowledge regarding the role of VEGF signaling in common oral diseases is expanding, new potential translational applications emerge. In the present narrative review we aim to explore the role of VEGF signaling in oral cancer and destructive periodontal inflammatory diseases, with emphasis in its translational applications as potential biomarkers and therapeutic targets.

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Lymphatic growth factors are expressed in human gingiva and upregulated in gingival fibroblasts after stimulation

Cited by 6 publications

References 51 publications

<p>Eldecalcitol Inhibits LPS-Induced NLRP3 Inflammasome-Dependent Pyroptosis in Human Gingival Fibroblasts by Activating the Nrf2/HO-1 Signaling Pathway</p>

<p>Eldecalcitol Inhibits LPS-Induced NLRP3 Inflammasome-Dependent Pyroptosis in Human Gingival Fibroblasts by Activating the Nrf2/HO-1 Signaling Pathway</p>

Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal

Vascular Endothelial Growth Factor: A Translational View in Oral Non-Communicable Diseases

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