Lymphatic flow in humans as indicated by the clearance of 125I-labeled albumin from the subcutaneous tissue of the leg

Fernández, María José Agüeros; Davies, W.T.; Owen, Gareth; Tyler, A.

doi:10.1016/0022-4804(83)90131-2

Cited by 14 publications

(6 citation statements)

References 7 publications

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“…In Eqs. 3 and 4, the product of fWL is defined as the slit area per unit membrane area and can be denoted as A o and the slit area per unit cleft depth as A o /Δ x 12,32. The diffusion coefficient contains a hindrance factor H ( λ ) that depends on the ratio λ of solute radius r s to slit half‐width W /2.…”

Section: Computational Model Frameworkmentioning

confidence: 99%

“…Fewer studies have been dedicated to lymphatic clearance, and to our knowledge no quantitative models have been developed. Published studies focus on determining the lymph flow rate by either cannulation of lymph ducts25–29 or by measuring the disappearance rate of large solutes from a specific site 30–33. Dermal capillary clearance was modeled by Kretsos et al5 as a uniform first‐order clearance through the blood capillaries into the systemic circulation.…”

Section: Introductionmentioning

confidence: 99%

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Dermal Clearance Model for Epidermal Bioavailability Calculations

Ibrahim

Nitsche

Kasting

2012

Journal of Pharmaceutical Sciences

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Section: Computational Model Frameworkmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dermal Clearance Model for Epidermal Bioavailability Calculations

Ibrahim

Nitsche

Kasting

2012

Journal of Pharmaceutical Sciences

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“…Empregando um protocolo semelhante ao deste trabalho, Andrade encontrou grande variabilidade na taxa de contagens da região inguinal entre os pacientes 15 . Esta variação também é relatada por outros autores 11,19,20 , e talvez seja, em parte, explicada por pequenas diferenças entre a atividade administrada em cada membro. Em nosso estudo, a análise da curva não possibilitou a distinção entre o membro com e sem linfedema, mesmo após correção pela atividade administrada, o que possivelmente indique a interferência de outros fatores, como a variação na retenção do material em trânsito por cada linfonodo ou por toda a cadeia inguinal.…”

Section: Resultsunclassified

“…Em nosso estudo, a análise da curva não possibilitou a distinção entre o membro com e sem linfedema, mesmo após correção pela atividade administrada, o que possivelmente indique a interferência de outros fatores, como a variação na retenção do material em trânsito por cada linfonodo ou por toda a cadeia inguinal. Diversos autores propõem que, ao invés da chegada do radiofármaco na cadeia inguinal, o clareamento do ponto de injeção seria um melhor parâmetro de quantificação [19][20][21][22] .…”

Section: Resultsunclassified

Critérios semiquantitativos de análise da linfocintilografia em linfedema dos membros inferiores

Sapienza¹,

Endo²,

Ferraro³

et al. 2006

J. vasc. bras.

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288 ResumoObjetivo: Avaliar se diferentes parâmetros de análise da linfocintilografia permitem a distinção entre membros com e sem linfedema em pacientes com quadro clínico de edema unilateral.Métodos: Levantamento retrospectivo dos exames de 20 pacientes submetidos a linfocintilografia para investigação de edema unilateral de membros inferiores, divididos em pacientes com linfedema primário (grupo 1, n = 7) ou secundário pós-traumático (grupo 2, n = 13). Foi realizada linfocintilografia após injeção subdérmica de soroalbumina humana marcada com tecnécio-99m nos pés, com imagens da região inguinal durante 15 min e imagens dos membros inferiores após 15 min e 1 h. Os parâmetros analisados foram: tempo de aparecimento da cadeia inguinal, índice semiquantitativo (fluxo linfático, difusão intersticial, aspecto dos vasos, tempo de aparecimento e aspecto dos linfonodos inguinais) e inclinação da curva de atividade em função do tempo da região inguinal. As medidas nos membros clinicamente acometidos foram comparadas aos membros sem linfedema em ambos os grupos.Resultado: O tempo médio de aparecimento da cadeia inguinal e o índice semiquantitativo foram significativamente maiores nos membros com linfedema que nos membros sem linfedema quando considerados todos os pacientes, com maior retardo e índice semiquantitativo nos membros com linfedema do grupo 1 em relação aos do grupo 2.Conclusão: A análise do tempo de aparecimento da cadeia inguinal e o índice semiquantitativo fornecem parâmetros objetivos de avaliação do linfedema que podem auxiliar na detecção e seguimento dos pacientes com linfedema.Palavras-chave: Cintilografia, linfedema. AbstractObjective: To evaluate whether different parameters could be used in the analysis of lymphoscintigraphy to distinguish affected from unaffected limbs in patients with unilateral edema.Methods: Twenty patients who underwent lymphoscintigraphy for screening of unilateral lower limb edema were retrospectively assessed and divided into patients with primary lymphedema (group 1, n = 7) or secondary post-traumatic edema (group 2, n = 13). Technetium 99m-labeled human serum albumin was subdermally administered in the feet, followed by a dynamic lymphoscintigraphy of the pelvis during 15 min and static images of the lower limbs after 15 min and 1 h. Analyzed parameters were detection time of groin activity, semi-quantitative index (lymph flow, interstitial diffusion, aspect of vessels, detection time and aspect of inguinal lymph nodes) and slope of groin time-activity curve. The parameters of clinically affected limbs were compared to those of the contralateral limbs in both groups.Results: Detection time of groin activity and semi-quantitative index were significantly higher in affected limbs considering all patients, with a more severe delay and a higher semi-quantitative index in group 1 compared to group 2.Conclusion: Analysis of detection time of groin activity and semiquantitative index can add objective parameters that can be used in the diagnosis and follow-up of patients with ly...

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“…The isotope clearance technique, which has been widely used in subcutaneous tissue, renders quantitative measurements of skin lymph flow possible [9, 10, 11, 12]. The reliability and reproducibility of this method have been demonstrated previously in the normal flank skin of anesthetized large white pigs [7, 13].…”

Section: Introductionmentioning

confidence: 99%

Measurement of the Lymphatic Clearance of the Human Skin Using a Fluorescent Tracer

Leu¹,

Husmann²,

Held³

et al. 2001

J Vasc Res

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The lymphatic clearance of the human skin at the instep of the foot was measured in 20 healthy volunteers (mean age ± SD 33.8 ± 10.5 years). Ten microliters of fluorescein isothiocyanate-dextran 150,000 were injected intradermally and the fluorescent light intensity of the deposit was measured 10 min and 24 h after injection by window densitometry. Fluorescent light intensity decreased by 31.2 ± 13.5 arbitrary units (p < 0.0001) or by a factor of 4.1 ± 3.9. Reproducibility was tested 2–6 weeks later in 7 subjects and an intraclass reliability of 0.76 was found. These are the first measurements of the lymphatic clearance of the human skin using a fluorescent tracer. The method is easier and safer than the isotope clearance technique and small areas of the human skin can be investigated. The data found form a basis with which to compare measurements made in patients with different forms of edema.

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Lymphatic flow in humans as indicated by the clearance of 125I-labeled albumin from the subcutaneous tissue of the leg

Cited by 14 publications

References 7 publications

Dermal Clearance Model for Epidermal Bioavailability Calculations

Dermal Clearance Model for Epidermal Bioavailability Calculations

Critérios semiquantitativos de análise da linfocintilografia em linfedema dos membros inferiores

Measurement of the Lymphatic Clearance of the Human Skin Using a Fluorescent Tracer

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