Lymphatic filariasis: Surveillance action among immigrants from endemic areas, Acre State, Brazilian Amazon

Nunes, Lucineide Valentin; Rocha, Abraham; Araújo, Julianna Siciliano de; Braga, Cynthia; Alcântara, Patrícia Pereira Tavares de; Fiorillo, Karina; Ximenes, Camila Viana; Brandão, Eduardo; Modesto, Claudia Dávila; Souza, Thayna Maria Holanda de; Brilhante, Andréia Fernandes

doi:10.1016/s2222-1808(16)61081-2

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…The two proteins share a 98% sequence similarity at the nucleotide level and both are homologous to equivalent SXP genes from related parasites, such as B. malayi (Bm14 and SXP-1), Onchocerca volvulus (Ov17) and Ascaris suum (As14) ( Pandiaraja et al 2010a ). The results described here confirm that ELISA assays based on Wb14 and on its WbT variant perform with similar sensitivity and greater specificity for W. bancrofti than the well-established BM14 CELISA test ( Weil and Ramzy 2007 , Weil et al 2011 , Nunes et al 2016 ). The Bm14 antigen was originally assayed with sera from individuals with LF from regions of India and Egypt, with 90% of the sera reactive against this antigen and cross-reactions seen only with O. volvulus ( Chandrashekar et al 1994 ).…”

Section: Discussionsupporting

confidence: 77%

“…Among those antigens, two of the best known are from W. bancrofti , WbSXP-1 ( Rao et al 2000 ) and Wb123 ( Kubofcik et al 2012 ) and one from B. malayi , Bm14 ( Lammie et al 2004 ). The latter gave rise to the most widely used antibody capture kit for LF diagnosis, the ELISA BM14 (CELISA) ( Weil and Ramzy 2007 , Weil et al 2011 , Nunes et al 2016 ). However, cross-reactivity issues have been reported with the ELISA BM14 test ( Weil et al 2011 ) and the fact that the recombinant antigen is derived from B. malawi , instead of W. bancrofti , may lead to some false negative results.…”

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confidence: 99%

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Evaluation of the recombinant antigens Wb14 and WbT for the capture antibody diagnosis of lymphatic filariasis

Pastor

Rocha

Cassemiro

et al. 2018

Mem. Inst. Oswaldo Cruz

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BACKGROUND Lymphatic filariasis (LF) is a parasitic disease caused mainly by the Wuchereria bancrofti worm and that affects up to 120 million people worldwide. LF is the second cause of chronic global deformity, responsible for 15 million people with lymphedema (elephantiasis) and 25 million men with scrotal hydrocele. Its diagnosis is still associated with numerous difficulties, such as the sample collection periods (microfilaria nocturnal periodicity) and limited diagnostic kits.OBJECTIVES The aim of this work was to evaluate two recombinant antigens (Wb14 and WbT) as part of an enzyme-linked immunosorbent assay (ELISA) based antibody capture tests for LF.METHODS The recombinant antigens rWb14 and rWbT were expressed in Escherichia coli BL21 and an antibody capture ELISA was performed. For this, sera were used from microfilaremic individuals with W. bancrofti (MF), chronic pathology (CP), individuals infected with Strongyloides (SP) and healthy controls from endemic (EN) and non-endemic (NE) areas.FINDINGS Both tests showed similar results, with 90% sensitivity and 96.6% specificity. In comparison with the BM14 ELISA commercial test, the Wb14 and WbT antigens performed with identical sensitivity but greater specificity. Reduced positivity with the CP suggested a potential to monitor cure. This was not confirmed, however, when sera from individuals up to seven years after treatment were assayed.MAIN CONCLUSIONS The Wb14 and WbT ELISAs were considered efficient and promising diagnostic tests. Due to the importance of antibody capture analysis to evaluate the Global Program to Eliminate Lymphatic Filariasis (GPELF), the tests proposed here appear as great alternatives to the available commercial system.

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Section: Discussionsupporting

confidence: 77%

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confidence: 99%

Evaluation of the recombinant antigens Wb14 and WbT for the capture antibody diagnosis of lymphatic filariasis

Pastor

Rocha

Cassemiro

et al. 2018

Mem. Inst. Oswaldo Cruz

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“…The possibility that highly mobile migrant workers from Myanmar introduced and maintained LF transmission in Thailand, after effective MDA throughout most of the latter country, resulted in additional rounds of MDA in border communities and sparked epidemiological research on the connectivity between communities (Bhumiratana et al, 2010;Satimai et al, 2011;Bhumiratana et al, 2013;Toothong et al, 2015;Dickson et al, 2017). The influx of thousands of refugees from Haiti, where LF is hyperendemic, to Brazil, where LF transmission has been reduced and even eliminated in some states, has prompted screening and treatment of recent immigrants to reduce risk of re-introduction (Nunes et al, 2016;Zuchi et al, 2017). Even in the remote islands of Samoa and American Samoa, models have shown that movement of people between countries could have a significant impact on the transmission of infectious diseases, including LF (Xu et al, 2018b).…”

Section: Lymphatic Filariasismentioning

confidence: 99%

Genomic Epidemiology in Filarial Nematodes: Transforming the Basis for Elimination Program Decisions

et al. 2020

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“…To test for CFA, the enzyme-linked immunosorbent assay (ELISA) Og4C3-ELISA (TropBio® Pty Ltd, Townsville, Queensland, Australia) [20] and AlereTM filariasis test strip (FTS) [2,21,22] were used. For IgG4 detection through ELISA, two recombinant antigens were used: Wb123 [23] and Bm14 [14,24,25].…”

Section: Lf Surveymentioning

confidence: 99%

Evaluation of lymphatic filariasis in endemic area of Brazil where mass drug administration is not required

Silva

Xavier

Silva

et al. 2019

Pathogens and Global Health

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Mass drug administration (MDA) is the main counter-transmission strategy of the Global Programme to Eliminate Lymphatic Filariasis. In endemic countries, there are areas where MDA is not required. However, there is no standard approach in these areas, and studies are important to evaluate the epidemiological status. This study aimed to investigate lymphatic filariasis and strategies developed for its control in an area where MDA is not required. Together with the 2018 morbidity evaluation, a survey was conducted using point-of-care immunochromatographic test-AD12 tests for diagnostic screening in an area where MDA is not required. The methodology also included desk research based on Health Department reports of the control activities for lymphatic filariasis during 2003-2016. Among the 934 cases investigated in 2018, there was a 0.64% prevalence of circulating filarial antigen positive, comprising five adults and one 2-year-old child. Six patients aged 39-63 years had filarial disease. Fourteen surveys have already been conducted as control activities, and since 2009, there have been no positive cases. This study showed that the prevalence of antigenemia decreased from 2.97% in 2003 to 0.64% in 2018. Moreover, the transmission of filariasis infection was under control in this area. Our study provides insights into the surveillance phase by identifying areas of low transmission and where MDA is not required. Although we have not identified cases of filarial infection, there is a need to provide services that will provide assist those already affected with morbidity and help reduce and prevent disability.

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Lymphatic filariasis: Surveillance action among immigrants from endemic areas, Acre State, Brazilian Amazon

Cited by 9 publications

References 26 publications

Evaluation of the recombinant antigens Wb14 and WbT for the capture antibody diagnosis of lymphatic filariasis

Evaluation of the recombinant antigens Wb14 and WbT for the capture antibody diagnosis of lymphatic filariasis

Genomic Epidemiology in Filarial Nematodes: Transforming the Basis for Elimination Program Decisions

Evaluation of lymphatic filariasis in endemic area of Brazil where mass drug administration is not required

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