Lymphatic Endothelial Tumors Induced by Intraperitoneal Injection of Incomplete Freund's Adjuvant

Mancardi, Sabrina; Stanta, Giorgio; Dusetti, Nelson; Bestagno, Marco; Jussila, Lotta; Zweyer, Marina; Lunazzi, Gian Carlo; Dumont, Daniel; Alitalo, Kari; Burrone, Óscar R.

doi:10.1006/excr.1998.4270

Cited by 65 publications

(107 citation statements)

References 22 publications

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“…Vegfr3 EGFPLuc females were treated with i.p. injection of (CFA) (44). A robust and transient increase in luciferase activity was observed in the abdominal area of CFA-treated females ( Fig.…”

Section: Imaging Of Lymphangiogenesis Induced By Specific Growth Factmentioning

confidence: 97%

In vivo imaging of lymphatic vessels in development, wound healing, inflammation, and tumor metastasis

Martínez-Corral

Olmeda

Diéguez-Hurtado

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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107

106

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Lymphatic vessel growth or lymphangiogenesis occurs during embryonic development and wound healing and plays an important role in tumor metastasis and inflammatory diseases. However, the possibility of noninvasive detection and quantification of lymphangiogenesis has been lacking. Here, we present the Vegfr3 EGFPLuc mouse model, where an EGFP-luciferase fusion protein, expressed under the endogenous transcriptional control of the Vegfr3 gene, allows the monitoring of physiological and pathological lymphangiogenesis in vivo. We show tracking of lymphatic vessel development during embryogenesis as well as lymphangiogenesis induced by specific growth factors, during wound healing and in contact hypersensitivity (CHS) -induced inflammation where we also monitor down-regulation of lymphangiogenesis by the glucocorticoid dexamethasone. Importantly, the Vegfr3-reporter allowed us to tracking tumor-induced lymphangiogenesis at the tumor periphery and in lymph nodes in association with the metastatic process. This is the first reporter mouse model for luminescence imaging of lymphangiogenesis. It should provide an important tool for studying the involvement of lymphangiogenesis in pathological processes.bioluminescence | fluorescence | knockin mouse

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Section: Imaging Of Lymphangiogenesis Induced By Specific Growth Factmentioning

confidence: 97%

In vivo imaging of lymphatic vessels in development, wound healing, inflammation, and tumor metastasis

Martínez-Corral

Olmeda

Diéguez-Hurtado

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

107

106

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“…The mouse blood vessel EC line 1G11, isolated from the lung, was provided by A. Vecchi (Milan, Italy) and cultured as previously described ( 15 ). The mouse LEC lines MELC ( 46 ) and SV-LEC ( 14 ) were provided by A. Vecchi (Milan, Italy) and J.S. Alexander (Shreveport, LA), respectively, and were cultured as previously described.…”

Section: Cellsmentioning

confidence: 99%

The adhesion molecule L1 regulates transendothelial migration and trafficking of dendritic cells

et al. 2009

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The adhesion molecule L1, which is extensively characterized in the nervous system, is also expressed in dendritic cells (DCs), but its function there has remained elusive. To address this issue, we ablated L1 expression in DCs of conditional knockout mice. L1-defi cient DCs were impaired in adhesion to and transmigration through monolayers of either lymphatic or blood vessel endothelial cells, implicating L1 in transendothelial migration of DCs. In agreement with these fi ndings, L1 was expressed in cutaneous DCs that migrated to draining lymph nodes, and its ablation reduced DC traffi cking in vivo. Within the skin, L1 was found in Langerhans cells but not in dermal DCs, and L1 defi ciency impaired Langerhans cell migration. Under infl ammatory conditions, L1 also became expressed in vascular endothelium and enhanced transmigration of DCs, likely through L1 homophilic interactions. Our results implicate L1 in the regulation of DC traffi cking and shed light on novel mechanisms underlying transendothelial migration of DCs. These observations might offer novel therapeutic perspectives for the treatment of certain immunological disorders.

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“…9 At the microscopic level, immunohistochemical staining for specific markers such as lymphatic vessel endothelial hyaluronan receptor-1 (Lyve1), vascular endothelial growth factor receptor 3 (VEGFR3) or Podoplanin (Pdpn) or PROX-1 (Prox1) provides an opportunity for visualization of lymphatic microvessels. [10][11][12][13] Examples of in vivo models manipulating lymphangiogenesis include (a) induction of tumor-associated lymphangiogenesis by VEGF-C or -D overexpression in tumor cells 8,14,15 or in transgenic mice, 16,17 (b) lymphatic hyperplasia induced by intraperitoneal injection of incomplete Freund's adjuvant, 18 (c) implantation of growth factor-containing pellets into corneal micropockets 19,20 and (d) the use of avian chorioallantoic membrane (CAM) to stimulate lymphangiogenesis. 21 Each assay has its own advantages and limitations depending on the objectives.…”

mentioning

confidence: 99%

A practical and sensitive method of quantitating lymphangiogenesis in vivo

Majumder

Xin

Lala

2013

Laboratory Investigation

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Lymphatic Endothelial Tumors Induced by Intraperitoneal Injection of Incomplete Freund's Adjuvant

Cited by 65 publications

References 22 publications

In vivo imaging of lymphatic vessels in development, wound healing, inflammation, and tumor metastasis

In vivo imaging of lymphatic vessels in development, wound healing, inflammation, and tumor metastasis

The adhesion molecule L1 regulates transendothelial migration and trafficking of dendritic cells

A practical and sensitive method of quantitating lymphangiogenesis in vivo

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