Lymphatic Drainage Mapping with Indirect Lymphography for Canine Mammary Tumors

Collivignarelli, Francesco; Tamburro, Roberto; Aste, Giovanni; Falerno, Ilaria; Signore, Francesca Del; Simeoni, Francesco; Patsikas, M. N.; Gianfelici, Jacopo; Terragni, Rossella; Attorri, Valeria; Carluccio, Augusto; Vignoli, Massimo

doi:10.3390/ani11041115

Cited by 22 publications

(37 citation statements)

References 30 publications

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“…Additionally, the combined use of ultrasonographic characteristics in malignancy prediction was not explored. Considering the lymphatic system’s importance for metastasis, future research should also include the draining lymph nodes and investigate the potential role of CEUS in the detection of metastases [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

B-Mode and Contrast-Enhanced Ultrasonography Aspects of Benign and Malignant Superficial Neoplasms in Dogs: A Preliminary Study

Hillaert

Stock

Duchateau

et al. 2022

Animals

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Contrast-enhanced ultrasonography (CEUS) is considered a promising technique for differentiation of benign and malignant tumors in humans. However, few studies have assessed superficial neoplasms in dogs by means of CEUS. The aim of this study was to identify ultrasonographic criteria evaluated by B-mode ultrasound (US) and CEUS that may be used to distinguish benign and malignant superficial neoplasms in dogs. A total of 63 superficial neoplasms from 59 dogs were evaluated using B-mode US and CEUS prior to histopathologic examination. Qualitative and quantitative parameters were compared between benign and malignant neoplasms by Fischer’s exact test or fixed effects model. With B-mode US, a significant difference was found for border definition, echogenicity and echotexture. With CEUS, a significant difference was found for the enhancement pattern at wash-in and the wash-out area under the curve at the center of the neoplasm. Malignant neoplasms had on average a lower regional blood volume during the wash-out phase compared to benign neoplasms. Despite these significant differences, there was a considerable overlap in B-mode and CEUS parameters between benign and malignant neoplasms. In conclusion, B-mode US and CEUS might contribute to malignancy prediction; however, based on individual ultrasonographic parameters, they seem unable to replace cytology or histopathology.

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Section: Discussionmentioning

confidence: 99%

B-Mode and Contrast-Enhanced Ultrasonography Aspects of Benign and Malignant Superficial Neoplasms in Dogs: A Preliminary Study

Hillaert

Stock

Duchateau

et al. 2022

Animals

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“…Absence of efficient prognosis protocols is related to complexity of interpretation of the data due to application of different methods of studying tumour components (Matos et al, 2012). Taking into account that metastases and relapses develop in around 35-70% of bitches after surgical treatment (Collivignarelli et al, 2021), evaluating the prognostic markers is the key element to improve the therapeutic strategies for treating cancer patients (Boracchi et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…In a multi-center retrospective study, Burrai et al (2020) demonstrated that the most significant factors of the prognosis are the size of the tumour and age of the animal. Presence of metastases in the regional lymph nodes is an important factor influencing the prediction and treatment in cases of mammary tumours (Collivignarelli et al, 2021). Yamagami et al (1996) observed no significant differences in the survival of bitches with different subtypes of adenocarcinomas (tubular, papillary and cystic papillary).…”

Section: Introductionmentioning

confidence: 98%

Prognostic markers of canine mammary tumours: Retrospective study of 142 cases

Kovalenko

Bilyi

Skliarov

et al. 2021

Regul. Mech. Biosyst.

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Due to relevance of the problem, prediction of biological behaviour of neoplasias in mammary glands of dogs requires using contemporary approaches to the study, first of all, of ways of dissemination of tumour cells. One of them is studying the mechanisms of migration of cancer cells out of the neoplasm tissues with further dissemination and development of metastatic sites in the regional lymphatic nodes and remote tissues. We studied the survival period of bitches with tumours of the mammary glands following regional or unilateral mastectomy. Among malignant mammary tumours in bitches, the most often diagnosed were single tumours (57.5%), which histologically were classified to carcinomas – ductal (26.9%) and mixed type (21.9%). Probability of intratumoral invasion to blood vessels equaled 12.0%, to lymph vessels – 7.8%, lymph nodes – 12.8%. It depends on the histological type of the tumour, the most aggressive potentially being сomedocarcinoma, tubulopapillary carcinoma and ductal carcinoma. Parameters of life expectancy and survival level after mastectomy depend on clinical stage of the disease (increase in the stage from the first to the third was characterized by decrease from 12.8 ± 9.5 to 9.4 ± 7.8 months), presence of angio/lymphatic invasions, presence of angiolymphatic invasion, but had no correlation with the size of the tumours. An important predictor of tumour-related death of dogs suffering neoplasias of the mammary glands is index vet-NPI, which has significant correlation with the clinical stage according to Owen and median survival. In particular, median survival in patients with the index lower than 4 exceeded the corresponding values in dogs with the index above 4 by 1.3 times. A promising direction of further research would be studying biological mechanisms of development of tumour emboli in the blood and lymph vessels, metastatic sites in lymph nodes, and also determining their role in pathogenesis of canine mammary tumours.

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“…The sentinel lymph node (SLN), defined as the first lymph node on the drainage pathway of a tumor, may differ from the anatomically closest regional lymph node (RLN) in 22–63% of tumor-bearing dogs [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ], and metastases to the SLN have been reported in up to 50–70% of dogs, depending on the tumor type [ 2 , 4 , 5 , 6 , 9 , 10 , 11 , 12 ]. Given the well-accepted impact of the accurate detection of nodal metastases on staging and treatment recommendations, the implementation of sentinel lymph node biopsy (SLNB) in the surgical management of canine malignancies has gained increased interest in recent years [ 8 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Sentinel Lymph Node Biopsy Is Feasible in Dogs with Scars from Prior Local Excision of Solid Malignancies

Gariboldi

Stefanello

Nolff

et al. 2022

Animals

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Sentinel lymph node (SLN) biopsy is a well-established staging tool in canine oncology. This study aims to explore the feasibility of SLN biopsy in dogs with scars from prior excised solid malignancies that were referred for further tumor staging and/or adjuvant treatment options. Mapping was either performed using radiopharmaceutical, methylene blue, and/or near-infrared fluorescent (NIRF) imaging. Thirty-three dogs with 34 scars from prior excision of the mast cell tumor (MCT) (n = 29), soft tissue sarcoma (n = 2), oral melanoma (n = 1), subungual melanoma (n = 1), and mammary adenocarcinoma (n = 1) were retrospectively enrolled. Primary treatment consisted of curative intent/wide tumor excisions in 50.0% of dogs and marginal excision in the remaining 50.0%. The median time between tumor excision and SLN biopsy was 50 days (range 17–110 days). The procedure was successful in 31/34 scars, translating to a detection rate of 91.2%. The SLN did not correspond to the regional lymph node in 19/31 scars (61.3%). SLN metastases were histologically identified in 13/31 (41.9%) dogs, all of them affected by MCT. Based on our results, SLN biopsy using lymphoscintigraphy/methylene blue and/or NIRF is feasible in dogs presenting with scars from the prior surgical excision of solid tumors, and should be suggested for accurate nodal staging.

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Lymphatic Drainage Mapping with Indirect Lymphography for Canine Mammary Tumors

Cited by 22 publications

References 30 publications

B-Mode and Contrast-Enhanced Ultrasonography Aspects of Benign and Malignant Superficial Neoplasms in Dogs: A Preliminary Study

B-Mode and Contrast-Enhanced Ultrasonography Aspects of Benign and Malignant Superficial Neoplasms in Dogs: A Preliminary Study

Prognostic markers of canine mammary tumours: Retrospective study of 142 cases

Sentinel Lymph Node Biopsy Is Feasible in Dogs with Scars from Prior Local Excision of Solid Malignancies

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